Navigation Links
Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc.

Introduction

Nitrofuran antibiotics are veterinary drugs. The use of these in food production is already banned in the United States, Australia, Canada, Japan, Singapore, Bangladesh, and the European Union because of a possible increased cancer-risk through long-term consumption.

Trace amounts of nitrofurans contamination has been found in warm-water prawns, shrimp, and chicken. The contamination may occur from deliberate, direct misuse of the drugs as well as from contaminated feed.

The analyses of nitrofurans are based on the detection of the metabolites of the parent drugs. Since the parent drugs are very rapidly metabolized and the tissue-bound metabolites are detectable for several weeks after administration, the metabolites are a much better marker for the detection of the abuse of these antibiotics.

The metabolites of furazolidone, furaltadone, nitrofurantoine and nitrofurazone are 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AH), and semicarbazide (SC), respectively. Nitrobenzaldehyde, the most commonly used derivatizing reagent for these metabolites, forms a nitrophenyl derivative for LC/MS analyses.

Instrumentation

Varian ProStar 430 AutoSampler

Varian ProStar 210 Isocratic Solvent Delivery Modules

Varian 1200L LC/MS equipped with ESI source

Materials and Reagents

A 5 pg/μL standard mixture of 2-nitrobenzyladehyde derivative of AOZ, AMOZ, AH, and SC was kindly provided by Thai Unique, Thailand.

All other chemicals were reagent grade or HPLC grade.

Results and Discussion

The LC method used a 10 minute run cycle. It is clear from the Multiple Reaction Monitoring (MRM)
'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:2/27/2015)... , Feb. 27, 2015  Pfenex Inc. (NYSE MKT: ... of biosimilar therapeutics, today announced that it will be ... Marquis on March 2 nd at 3pm PST. Patrick ... a panel discussing the current state of the biosimilar ... . For more information on CALBIO ...
(Date:2/27/2015)...   PureTech , a science and technology R&D ... and technologies in the healthcare sector, announced today the ... Member of the Board of Directors of Sanofi, to ... been a pleasure to know Chris for many years ... working together more closely now," said Dr. Robert ...
(Date:2/26/2015)... - SQI Diagnostics Inc. ("SQI" or the "Company") (TSX-V: ... and commercializes proprietary technologies and products for advanced microarray ... the fiscal first quarter ended December  31, 2014. All ... (CAD), unless otherwise stated. "During the ... our existing customers and add to our sales pipeline," ...
(Date:2/26/2015)... DuPont Executive Vice President James C. ... advancements and product launches across the Agriculture and Nutrition ... Lynch 2015 Global Agriculture Conference. "While ... the last two years, long-term demand for agricultural production ... last decade, when demand for corn and soybeans increased ...
Breaking Biology Technology:Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 2Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 3Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 4SQI Diagnostics Reports First Quarter Financial Results 2SQI Diagnostics Reports First Quarter Financial Results 3SQI Diagnostics Reports First Quarter Financial Results 4SQI Diagnostics Reports First Quarter Financial Results 5SQI Diagnostics Reports First Quarter Financial Results 6DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 2DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 3DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 4DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 5
... Company CEO is featured speaker: "Case Study - Screening ... Safety and Efficacy" on Friday, September 26 ... feature its expanding,line of ion channel services and cell ... the Assays and Cellular Targets,conference in San Diego, September ...
... today announced the publication of its first peer-reviewed ... Experiments (JoVE).,This makes Sigma-Aldrich the first life science ... scientific publication process. Peer-reviewed,video protocols are an innovative ... scientific communication and with it the speed of ...
... 17 /PRNewswire-FirstCall/ - On August 20, 2008,Oncothyreon Inc. (Nasdaq: ... received a,letter from The Nasdaq Stock Market indicating that ... listing on The NASDAQ Global Market,because it did not ... among other things, that the market value of,its common ...
Cached Biology Technology:ChanTest to Highlight the World's Largest Catalog of Ion Channel-Expressing Cell Lines and Discovery Services at Assays & Cellular Targets Conference, Booth 11 2Sigma-Aldrich(R) Becomes First Life Science Company to Publish in Peer-Reviewed Video Journal JoVE 2Sigma-Aldrich(R) Becomes First Life Science Company to Publish in Peer-Reviewed Video Journal JoVE 3Oncothyreon announces receipt of NASDAQ letter regarding compliance with NASDAQ Global Market listing standards 2Oncothyreon announces receipt of NASDAQ letter regarding compliance with NASDAQ Global Market listing standards 3
(Date:2/5/2015)... PARK, N.C. , Feb. 5, 2015  Marken ... specialist logistics company and has launched a new marketing ... Clinical Logistics Organization (CLO).  The new campaign focuses on ... on patient, protocol and shipments. The first ... , aligns Marken,s priorities with its client,s priorities. ...
(Date:2/5/2015)... , Jan. 27, 2015   ... of globally deployed, innovative test solutions for ... the delivery of its TS-323 GENASYS ... Systems Company (LMSSC). GENASYS is a high-performance ... of mission-critical applications that require performance functional ...
(Date:2/5/2015)... Research and Markets ( http://www.researchandmarkets.com/research/6lngtl/global_biometrics ... "Global Biometrics Market (2014-2020): Market Forecast By Technologies, ... to their offering. , In ... is anticipated to overtake North America ... spending towards IT security, government biometric based projects ...
Breaking Biology News(10 mins):Marken Launches New Patient-Centric Campaign 2Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 2Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 3Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 4Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 2Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 3
... had two or more induced abortions have a reduced risk ... to what degree physical activity during pregnancy protects against pre-eclampsia, ... studies from the Norwegian Institute of Public Health (NIPH) that ... (MoBa). The new results from MoBa were presented ...
... It is well known that plants growing under unfavourable conditions ... it is estimated that in the US, abiotic stress reduces ... A spectacular example of the effect of stress in ... by bonsai trees, in which every aspect of their stature, ...
... State University has made a breakthrough that could lead to ... implants. The researchers have found that the unique properties of ... that can be implanted into the human body including ... can scrub impurities from the blood. Researchers have long ...
Cached Biology News:Previous abortions and exercise: Do they affect pregnancy? 2Previous abortions and exercise: Do they affect pregnancy? 3The bonsai effect: Wounded plants make jasmonates, inhibiting cell division, stunting growth 2NC State finds new nanomaterial could be breakthrough for implantable medical devices 2
Rabbit polyclonal to FANCG ( Abpromise for all tested applications). Antigen: Synthetic peptide: MSRQTTSVGSSC, corresponding to amino acids 1 - 12 of Human FANCG. Entrez GeneID: 2189 Swis...
...
Rabbit polyclonal to DcR2 ( Abpromise for all tested applications). entrezGeneID: 8793 SwissProtID: Q9UBN6...
... Polyclonal to Human CELSR3 CELSR3 is an ... In mouse, this gene is expressed during development ... mice and rats in brain, spinal cord, dorsal ... from human B-cell/lung/testis, blood, brain, colon, heart/melanocyte/uterus, lung, ...
Biology Products: