Navigation Links
Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc.

Introduction

Nitrofuran antibiotics are veterinary drugs. The use of these in food production is already banned in the United States, Australia, Canada, Japan, Singapore, Bangladesh, and the European Union because of a possible increased cancer-risk through long-term consumption.

Trace amounts of nitrofurans contamination has been found in warm-water prawns, shrimp, and chicken. The contamination may occur from deliberate, direct misuse of the drugs as well as from contaminated feed.

The analyses of nitrofurans are based on the detection of the metabolites of the parent drugs. Since the parent drugs are very rapidly metabolized and the tissue-bound metabolites are detectable for several weeks after administration, the metabolites are a much better marker for the detection of the abuse of these antibiotics.

The metabolites of furazolidone, furaltadone, nitrofurantoine and nitrofurazone are 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AH), and semicarbazide (SC), respectively. Nitrobenzaldehyde, the most commonly used derivatizing reagent for these metabolites, forms a nitrophenyl derivative for LC/MS analyses.

Instrumentation

Varian ProStar 430 AutoSampler

Varian ProStar 210 Isocratic Solvent Delivery Modules

Varian 1200L LC/MS equipped with ESI source

Materials and Reagents

A 5 pg/μL standard mixture of 2-nitrobenzyladehyde derivative of AOZ, AMOZ, AH, and SC was kindly provided by Thai Unique, Thailand.

All other chemicals were reagent grade or HPLC grade.

Results and Discussion

The LC method used a 10 minute run cycle. It is clear from the Multiple Reaction Monitoring (MRM) data produced that the 1200L LC/MS system can qualitatively and quantitatively detect nitrofurans to the ultra trace levels required. 10 mM ammonium acetate was used in the sample solution to reduce formation of sodium adducts and to enhance the detection limit for the product ions.

The Limit of Detection (LOD) for SC, AOZ, AH, and AMOZ is about 20 fg/μL*, 10 fg/μL, 60 fg/μL, and 10 fg/μL in solution, respectively. The linearity of the detector response was excellent (Figure 1). The R2 for 209>166, 236>134, 249>134 and 335>291 are 0.999, 0.998, 0.999 and 0.999, respectively. Typical chromatograms obtained are given in Figure 2.

Conclusion

The Varian 1200L LC/MS/MS system demonstrates excellent performance for the analyses of nitrofurans metabolites and is able to assist in the detection of potentially harmful substances in food imports/exports to improve food safety throughout the world.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/15/2017)... ... August 15, 2017 , ... The Conference Forum and The ... through a series of upcoming panels and events. The partnership culminates with the ... Hotel in New York City. , “With our experience in producing the Immuno-Oncology 360° ...
(Date:8/14/2017)... Linda, Ca (PRWEB) , ... August 14, 2017 ... ... for poorly characterized and performing antibodies. Key researchers in the antibody community have ... proper characterization and consistency for antibodies in the laboratory. , The ...
(Date:8/11/2017)... 11, 2017  Market researcher Kalorama Information ... article regarding the telemedicine market.  The telemedicine ... Information.  The article, "Heart and Asthma ...  used information from Kalorama Information,s Remote Patient ... Market  (Sleep, Diabetes, Vital Signs /EKG ...
(Date:8/11/2017)... ... August 11, 2017 , ... “There is an ... natural alternatives to synthetic ingredients,” said Matt Hundt, President of Third Wave Bioactives. ... manufacturing presence and know-how of Biorigin will allow us to bring truly novel ...
Breaking Biology Technology:
(Date:4/19/2017)... April 19, 2017 The global ... landscape is marked by the presence of several large ... held by five major players - 3M Cogent, NEC ... accounted for nearly 61% of the global military biometric ... in the global military biometrics market boast global presence, ...
(Date:4/13/2017)... India , April 13, 2017 According to ... Proofing, Identity Authentication, Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, ... MarketsandMarkets™, the IAM Market is expected to grow from USD 14.30 Billion ... Growth Rate (CAGR) of 17.3%. ... MarketsandMarkets ...
(Date:4/11/2017)... -- NXT-ID, Inc. (NASDAQ:   NXTD ) ("NXT-ID" ... of independent Directors Mr. Robin D. Richards and ... furthering the company,s corporate governance and expertise. ... Gino Pereira , Chief Executive Officer ... guidance and benefiting from their considerable expertise as we move ...
Breaking Biology News(10 mins):