Navigation Links
Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc.

Introduction

Nitrofuran antibiotics are veterinary drugs. The use of these in food production is already banned in the United States, Australia, Canada, Japan, Singapore, Bangladesh, and the European Union because of a possible increased cancer-risk through long-term consumption.

Trace amounts of nitrofurans contamination has been found in warm-water prawns, shrimp, and chicken. The contamination may occur from deliberate, direct misuse of the drugs as well as from contaminated feed.

The analyses of nitrofurans are based on the detection of the metabolites of the parent drugs. Since the parent drugs are very rapidly metabolized and the tissue-bound metabolites are detectable for several weeks after administration, the metabolites are a much better marker for the detection of the abuse of these antibiotics.

The metabolites of furazolidone, furaltadone, nitrofurantoine and nitrofurazone are 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AH), and semicarbazide (SC), respectively. Nitrobenzaldehyde, the most commonly used derivatizing reagent for these metabolites, forms a nitrophenyl derivative for LC/MS analyses.

Instrumentation

Varian ProStar 430 AutoSampler

Varian ProStar 210 Isocratic Solvent Delivery Modules

Varian 1200L LC/MS equipped with ESI source

Materials and Reagents

A 5 pg/μL standard mixture of 2-nitrobenzyladehyde derivative of AOZ, AMOZ, AH, and SC was kindly provided by Thai Unique, Thailand.

All other chemicals were reagent grade or HPLC grade.

Results and Discussion

The LC method used a 10 minute run cycle. It is clear from the Multiple Reaction Monitoring (MRM)
'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:4/16/2015)... Portage Biotech Inc. ("Portage" or "the Company" )) (OTC ... announce that the Company will be presenting  at the ... held in London, UK . Portage,s ... James Mellon , a director of Portage, will provide ... at its subsidiaries, Portage Pharmaceuticals Ltd. ("PPL"), and Biohaven ...
(Date:4/16/2015)... , April 16, 2015 ... the Russian pharmaceutical investment and R&D group, ... collaboration for research, development and commercialization of ... with the intention to stimulate the ...      (Logo: http://photos.prnewswire.com/prnh/20150416/739742-a) ,     (Logo: ...
(Date:4/16/2015)... BURLINGAME, Calif. , April 16, 2015  Scientists ... session, "New Drugs on the Horizon," at the American ... Philadelphia, PA on Sunday, April 19, ... , Ph.D., Cleave,s Director of Biology, will present new ... CB-5083, a first-in-class, oral inhibitor of p97, a critical ...
(Date:4/16/2015)... Switzerland , April 16, ... part of Debiopharm Group ™ , a Swiss-based ... target unmet medical needs as well as ... on the investigational compounds Debio 1143 (SMAC mimetic) ... presented at the 2015 Annual meeting of ...
Breaking Biology Technology:Portage to Present at Master Investor 2015 in London, UK 2Portage to Present at Master Investor 2015 in London, UK 3Portage to Present at Master Investor 2015 in London, UK 4Lancet and ChemRar High Tech Center Have Announced a Partnership in the Development and Production of Novel Drugs in Russia 2Cleave Biosciences to Participate in Special Session "New Drugs on the Horizon" at AACR Annual Meeting 2015 2Cleave Biosciences to Participate in Special Session "New Drugs on the Horizon" at AACR Annual Meeting 2015 3Debiopharm International SA Announces Results of 2 Key Oncology Programs at 2015 AACR Conference 2Debiopharm International SA Announces Results of 2 Key Oncology Programs at 2015 AACR Conference 3
... producing tech-savvy workers and managers for Wisconsins knowledge-based ... It will require partnerships between business, education and ... the K-through-gray learning spectrum. , ,The latest example ... by UW-Stout to streamline transfer of credits for ...
... health care software and services company, announced Tuesday the ... The updated product includes integration with FUSION PACS, allowing ... to run from a single, unified end-user desktop application. ... improvements for the radiologist and technologist by eliminating the ...
... to reexamine the federal policy on stem cell research. ... of this debate, because our state is the birthplace of ... Jamie Thomson at the University of Wisconsin-Madison announced that ... isolated stem cells from human embryos and grown them in ...
Cached Biology Technology:Higher ed cooperation will produce more skilled workers for Wisconsin 2Higher ed cooperation will produce more skilled workers for Wisconsin 3Merge eFilm announces release of FUSION RIS version 2.5 2Gov. Doyle comments on unlocking the power of power of stem cell research 2Gov. Doyle comments on unlocking the power of power of stem cell research 3
(Date:4/2/2015)... -- At its 2015 ACMG Annual Clinical Genetics Meeting ... American College of Medical Genetics and Genomics (ACMG) announced ... Members of the ACMG Board of Directors serve as ... its policies and programs. ACMG is the national organization ... eventful time in medical genetics and genomics.  We are ...
(Date:3/31/2015)...  Elephant Talk Communications Corp. (NYSE MKT: ETAK) ("Elephant ... Defined Network Architecture (ET Software DNA® 2.0) platforms and ... $20.4 million for the year ended December 31, 2014 ... year ended December 31, 2013. At ... its multi-year transition away from its legacy landline business ...
(Date:3/24/2015)... 24, 2015 Research and Markets ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ) ... - Global Strategic Business Report" report to their offering. ... Biometrics in US$ Thousands by the following Segments: Face Biometrics, and ... Canada , Japan , ... Middle East & Africa , and ...
Breaking Biology News(10 mins):American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6
... (Alternext: ALEHT), a biotech company focused on personalized medicine, ... to announce the successful completion of a project dedicated ... additional strategic preclinical animal model species. To answer ... and the expanding interest in developing biomarkers and molecular ...
... 25 Dallas schools will spend July 18-29 dusting for ... Crime Scene Investigation (CSI) Camp, through a program sponsored ... Methodist University,s (SMU) CSI Summer Camp, which began this ... school students to science, technology, engineering and mathematics (STEM) ...
... Birds use their bills largely to forage and eat, and ... bird,s bill. But the bill can play an important role ... radiator for excess heat. A team of scientists have found ... strong influence in determining bill size in some birds, particularly ...
Cached Biology News:ONR-funded STEM program offers peek into world of real 'CSI' 2It's no sweat for salt marsh sparrows to beat the heat if they have a larger bill 2
... Fixed clamp trays ... easy Magnetic drive ... rpm 25 mm amplitude ... 25 ml to 2000 ml, including ...
... fluorescent microscopy-based imaging system that accurately counts ... calculates cell viability, in a single test. ... variability associated with the subjective interpretation of ... EasyCount ViaSure Kit, up to six tests ...
... blotting. Get results sooner and save hours of ... Eliminates the need for a membrane transfer step ... are ready-to-use. No reagents to prepare or mix ... required. Guarantees a simple to perform, mistake-free detection ...
... CHEF-DR III variable angle system, 220-240 V, is ... 6 Mb DNA fragments by pulsed field gel ... power module, variable-speed pump, 14 x 13 cm ... 15-well, 1.5 mm thick comb, screened cap, disposable ...
Biology Products: