Navigation Links
Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc.

Introduction

Nitrofuran antibiotics are veterinary drugs. The use of these in food production is already banned in the United States, Australia, Canada, Japan, Singapore, Bangladesh, and the European Union because of a possible increased cancer-risk through long-term consumption.

Trace amounts of nitrofurans contamination has been found in warm-water prawns, shrimp, and chicken. The contamination may occur from deliberate, direct misuse of the drugs as well as from contaminated feed.

The analyses of nitrofurans are based on the detection of the metabolites of the parent drugs. Since the parent drugs are very rapidly metabolized and the tissue-bound metabolites are detectable for several weeks after administration, the metabolites are a much better marker for the detection of the abuse of these antibiotics.

The metabolites of furazolidone, furaltadone, nitrofurantoine and nitrofurazone are 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AH), and semicarbazide (SC), respectively. Nitrobenzaldehyde, the most commonly used derivatizing reagent for these metabolites, forms a nitrophenyl derivative for LC/MS analyses.

Instrumentation

Varian ProStar 430 AutoSampler

Varian ProStar 210 Isocratic Solvent Delivery Modules

Varian 1200L LC/MS equipped with ESI source

Materials and Reagents

A 5 pg/μL standard mixture of 2-nitrobenzyladehyde derivative of AOZ, AMOZ, AH, and SC was kindly provided by Thai Unique, Thailand.

All other chemicals were reagent grade or HPLC grade.

Results and Discussion

The LC method used a 10 minute run cycle. It is clear from the Multiple Reaction Monitoring (MRM)
'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:9/11/2014)... a one atom thick sheet of carbon, can be ... form of graphene that could have a significant impact ... paint, it could provide an ultra-strong, non-corrosive coating for ... oxide solutions can be used to paint various surfaces ... After a simple chemical treatment, the resulting coatings behave ...
(Date:9/11/2014)... PA (PRWEB) September 11, 2014 ... conferee registration is now open for Pittcon ... for laboratory science. The event will be held ... Center, New Orleans, Louisiana. , The discounted ... 20, 2015. ($350 after 2/20/2015). This affordable price ...
(Date:9/11/2014)... - Portage Biotech Inc. ("Portage" or "the Company") (OTC: ... intends to conduct a Private Placement to raise ... preclinical development activities and financial commitments to Biohaven ... Company is proposing to complete a non-brokered offering ... a price of US$0.10 per Share, for gross ...
(Date:9/11/2014)... Awarded annually by Germany’s Design Zentrum Nordrhein-Westfalen, the ... label for excellent design. METTLER TOLEDO is extremely ... Dot award in recognition of high design ... , The Quantos Handheld Powder Dispenser (HPD) ... applications involving free-flowing powders. Forget the delicacy and ...
Breaking Biology Technology:Graphene paints a corrosion-free future 2Conferee Registration Opens for Pittcon 2015; Housing & Travel Open for Reservations 2Portage Announces Proposed Private Placement 2Portage Announces Proposed Private Placement 3Portage Announces Proposed Private Placement 4METTLER TOLEDO Handheld Powder Receives Red Dot Award for Product Design 2
... ... ... var shortURL ... we need to grab the first one. for (var r in data.results) { first_result = data.results[r]; break; } for ...
... ... ... var shortURL = ""; BitlyCB.alertResponse ... grab the first one. for (var r in data.results) { first_result = data.results[r]; break; } for (var key in ...
... in a row, a pair of Rensselaer students took ... held by the American Society of Mechanical Engineers (ASME). ... Jaron Kuppers won top honors in the national competition ... process. The SET process offers a new method for ...
Cached Biology Technology:YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 2YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 3YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 4YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 5YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 6YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 7YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 8YM BioSciences Expands Phase I/II Trial for JAK Inhibitor CYT387 9Medivir: R&D Day 2Medivir: R&D Day 3Medivir: R&D Day 4Medivir: R&D Day 5Medivir: R&D Day 6Medivir: R&D Day 7Medivir: R&D Day 8Rensselaer mechanical engineers win first place at ASME student manufacturing design competition 2Rensselaer mechanical engineers win first place at ASME student manufacturing design competition 3Rensselaer mechanical engineers win first place at ASME student manufacturing design competition 4
(Date:9/11/2014)... from the Rice Research Institute of Iran have ... from Europe and Asia, has changed its host preferences in ... importance of this adaptation for biological control of problematic weeds ... new host plant have been described in the open access ... of G. permixtana have been so far ...
(Date:9/11/2014)... of beetles in the genus Calathus , 17 of ... Ethiopian Highlands. Now scientists have found two new ones ... and have described them in Annals of the Entomological ... named for Juan Novoa, the son of one of the ... Adults are black and shiny, and are 9.5-11.5 millimeters long. ...
(Date:9/11/2014)... world and a concomitant upgrade of health care systems, ... a consequence, worldwide demand for agricultural products is also ... other important resources for seven billion humans. , ... wheat, maize, rice and vegetables. But as the ... to decline, the future ability to meet the world,s ...
Breaking Biology News(10 mins):Unusual host preference of a moth species could be useful for biological control 2Crop improvement and resistance to pathogens benefits from non-coding RNA studies 2Crop improvement and resistance to pathogens benefits from non-coding RNA studies 3
... A new study conducted in Bangladesh finds that ... levels in individuals chronically exposed to arsenic-contaminated drinking ... is naturally present in some soils and water. ... public health problem in at least 70 countries, ...
... projected increases in the use of corn for ethanol ... considerable, and water supply problems at the regional and ... from the National Research Council. The committee that ... for new agricultural techniques and technologies to help minimize ...
... study by Kevin Pope of Geo Eco Arc Research ... into the migration of anatomically modern humans out of ... present (BP). The comprehensive review of human ... supports the hypothesis, originally based largely on genetic evidence, ...
Cached Biology News:Folic acid lowers blood arsenic levels in Bangladesh 2Folic acid lowers blood arsenic levels in Bangladesh 3Increase in ethanol production from corn could significantly impact 2Increase in ethanol production from corn could significantly impact 3Environmental setting of human migrations in the circum-Pacific Region 2
...
... Lyophilized. Lyophilized from 2 mM sodium ... human liver. Cathepsin D is a major ... as a 52 kDa proenzyme. Overexpression of ... associated with higher risk of relapse and ...
... Grade Thrombin is a highly purified preparation ... with appropriate vectors. The preparation is functionally ... is free of detectable contaminating proteases. Prepared ... by certified tests to be negative for ...
Rat Hepatocytes (RH) (>3,000,000 cells)...
Biology Products: