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Analysis of Blood Serum on the Liberty Series II ICP-AES with the axially-viewed plasma

in blood serum diluted by a factor of 20.

The peak to the left of Cu (327.396 nm) is Sc and causes no problems as it is completely resolved. The tail of the OH peak does contribute slightly to the signal of the Cu (327.396 nm) but this has been successfully corrected using polynomial plotted background correction.

There is also the option of using the 327.396 nm line in the 2nd order where the resolution will be improved by a factor of 2. The sensitivity of the line is only reduced by approximately 30% when using it in second order and the peaks are completely resolved.

The Cu 327.396 nm line in the 1st order was selected for the analysis.

Blood serum analysis

The analysis consisted of a single Seronorm Trace Elements Serum, batch no. 311089, that was diluted 100-fold for the determination of Ca, Mg, Na and K, 20-fold for the determination of Ca, Mg, Na, K, Cu, Fe, P, S and Zn and 5-fold for the determination of Al and Mn. The elements Ca, Mg Na and K were determined in the 100 and 20-fold dilution blood serum solutions because any variation in the result would indicate the presence of ionization interference.

The analysis was repeated with the addition of Cs as an ionization buffer. The Cs was added by pumping CsCl solution (2% w/v Cs) into a T piece just before the nebulizer

Blood serum contains high levels of sodium and the potential for ionization interference is high. Matrix matching, such as having equal amounts of Na in all solutions, would mean that any signal enhancement or suppression because of ionization interference would be the same for all solutions. To measure major, minor and trace levels of elements in blood would then require multiple analyses because blood serum solutions of various dilution factors would be necessary with matrix matched standards to be prepared for each. The aim of this work was to show that
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