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Analysis of Blood Serum on the Liberty Series II ICP-AES with the axially-viewed plasma

ution with cesium being added.

The measured concentrations of Zn and the trace elements Mn and Al without the addition of cesium were similar to the certified values. With the addition of cesium, a slight increase in the measured concentrations of Zn and Mn were found. Although the results for Zn and Mn were slightly higher, they still compare well with the certified values.

No certified value was available for P and S in the blood serum batch that was used for the analysis. The measured results for P and S were however, very close to the certified values of another Seronorm Trace Elements Serum batch. The certified concentrations of the other elements for both serum batches varied only slightly and, therefore, the same could be assumed for P and S. These two elements did not appear to be affected by the presence of EIE as similar results were found with and without the addition of Cs.

Long term stability

No extensive evaluation of the long term stability was done with the microconcentric nebulizer. It appeared to operate well with no blockage being evident. Blood serum solutions diluted by a factor of 5 were aspirated continuously for periods of more than 30 minutes with no blockage being observed. Blood serum diluted 2-fold appeared to cause no problems.

A single long term stability run was done by continuously aspirating a 20-fold dilution blood serum solution and measuring the signal for a number of elements at intervals. The reproducibility of the measurements for Ca, Cu, Fe, Mg, Na, S and Zn over one hour ranged between 0.6 and 1.0 %RSD.

The replicate precision using a 3 second integration time and measuring 3 replicates ranged between 0.1 and 1.7 %RSD for all elements.

Summary

The concentrations of major, minor and trace levels of elements in blood serum were determined in a single analysis
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