Navigation Links
Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

oftware, where the x-axis was modified to reflect the percent denaturant along the length of the lane.


Results
Replicate samples collected from the same location exhibited consistently similar DGGE profiles, demonstrating the degree of reproducibility of the results (Figure 1). DGGE banding patterns exhibited some similarities among sites, but the method was sensitive enough to detect some differences among sites. Analysis of the number of bands, the number of unique site-specific bands, and similarity in banding patterns provided a relatively complete representation of differences among sites.

In some cases, bands detected were less well defined than other bands and appeared fuzzy on the gel images and as broad peaks on the gel profile graphs. Selection of PCR primers that amplify a smaller DNA fragment and use of a GC clamp is recommended to correct this problem.


Discussion
DGGE analysis of PCR products demonstrated that in many ways bacterial assemblages differed among sites. In general, more total and more unique bands were detected at the polluted sites. Other methods used to examine the bacterial assemblages at these same sites also revealed site-specific differences.5

Interpretation of DGGE results should be performed with the knowledge that DGGE provides a relative measure of species diversity. As was the case in this study, DGGE was intimately dependent on the efficiency of the DNA extraction procedure and on an unbiased high fidelity PCR. To more accurately understand the roles of bacterial populations to natural environments, it is necessary to be able to measure the microbial community structure
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:1/15/2014)... This webinar will focus on EMA and ... in biosimilars. , Regulatory frameworks are evolving many countries ... the complex nature of biopharmaceuticals makes the demonstration of ... challenging. Based on the specific aspects of biosimilar drug ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 Two champions ... continued their sponsorship of an annual competition for middle ... and access to, innovative STEM study. The competition presents ... TEAMS: Tests of Engineering Aptitude, Mathematics, and Sciences ...
(Date:1/15/2014)... 2013 was a banner year of continued innovation ... continued independent research led by the team at Wake ... million grant from the Susanne Marcus Collins Foundation, had ... reviewed journal, Amy Grant highlighted Brainwave Optimization® in First ...
(Date:1/15/2014)... 2014 TaiGen Biotechnology Company, Limited ("TaiGen") today announced ... a leading Russian pharmaceutical company, to develop and commercialize ... Federation , Turkey and ... a novel antibiotic for the treatment of bacterial infections ...
Breaking Biology Technology:Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4
... February 28, Evotec AG (Frankfurt Stock Exchange: EVT) ... study for EVT 302 in smoking,cessation, a reversible ... This double-blind, three-way cross-over study is designed ... and withdrawal symptoms,after short-term deprivation of cigarettes in ...
... Anadys Pharmaceuticals,Inc. (Nasdaq: ANDS ) announced today ... Options in Healthcare Conference on Tuesday,March 4, 2008 at ... being,held at the W Hotel in New York., ... Anadys,Pharmaceuticals, Inc., will present an overview of Anadys and ...
... time, researchers at North Carolina State University have ... physically or chemically different so-called Janus particles ... alternating electrical field is applied to liquid surrounding ... published in the Feb. 8, 2008, edition of ...
Cached Biology Technology:Evotec Starts Phase II in Smoking Cessation with EVT 302 2Anadys Pharmaceuticals to Present at the Susquehanna SIGnificant Options in Healthcare Conference 2'2-faced' particles act like tiny submarines 2
(Date:7/9/2014)... derived from the oils produced by algae may offer ... this goal, optimization of cost effective strategies for large-scale ... needed. Sapphire Energy has developed an innovative solution to ... systems, described in Industrial Biotechnology , a peer-reviewed ... is available on the Industrial Biotechnology ...
(Date:7/9/2014)... about the prevalence of antibiotic-resistant illness, Case Western ... to disabling disease: blocking bacteria,s access to iron ... bacterial siderophore, a small molecule, captures iron from ... as well as how the body launches ... findings appear in a recent edition of ...
(Date:7/9/2014)... usually announce themselves with pain and fever but often can ... that are sneaky and hard to beat. Now, scientists have ... of tiny DNA pyramids. Published in the journal ACS ... can flag bacteria and kill more of them than medicine ... some infectious pathogens can lie in wait, undetectable in the ...
Breaking Biology News(10 mins):Controlling contamination in open algae ponds for biofuels 2Bacteria hijack plentiful iron supply source to flourish 2
... genes significantly affect variation in voter turnout, shedding new ... in the political system. The research, conducted by ... UC San Diego) and psychologist Laura A. Baker (of ... of the American Political Science Review , a ...
... from research groups around Australia, the report includes chapters ... focus on steps that can be taken to adjust ... to the Farm Writers Association of NSW in Sydney ... Howden, said it was time for agriculture to start ...
... through a tropical or temperate forest immediately impresses us ... plant world, but our knowledge of how plants are ... Now, with data emerging from many genome sequencing projects, ... architecture at the molecular level. This knowledge has ...
Cached Biology News:Why do people vote? Genetic variation in political participation 2How to build a plant 2How to build a plant 3
... the needs of the biopharmaceutical ... supports fast cell growth rates ... maintaining high cell viability and ... the Baculovirus Expression Vector System ...
Mol wt: average mol wt12,360.96 Da by calculation...
A set of five proteins which can be used to calibrate a Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer....
Originally developed for the culture of Drosophila; suitable for culture of other dipteran cell lines. Powder, Insect cell culture tested...
Biology Products: