Navigation Links
Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

oftware, where the x-axis was modified to reflect the percent denaturant along the length of the lane.


Results
Replicate samples collected from the same location exhibited consistently similar DGGE profiles, demonstrating the degree of reproducibility of the results (Figure 1). DGGE banding patterns exhibited some similarities among sites, but the method was sensitive enough to detect some differences among sites. Analysis of the number of bands, the number of unique site-specific bands, and similarity in banding patterns provided a relatively complete representation of differences among sites.

In some cases, bands detected were less well defined than other bands and appeared fuzzy on the gel images and as broad peaks on the gel profile graphs. Selection of PCR primers that amplify a smaller DNA fragment and use of a GC clamp is recommended to correct this problem.


Discussion
DGGE analysis of PCR products demonstrated that in many ways bacterial assemblages differed among sites. In general, more total and more unique bands were detected at the polluted sites. Other methods used to examine the bacterial assemblages at these same sites also revealed site-specific differences.5

Interpretation of DGGE results should be performed with the knowledge that DGGE provides a relative measure of species diversity. As was the case in this study, DGGE was intimately dependent on the efficiency of the DNA extraction procedure and on an unbiased high fidelity PCR. To more accurately understand the roles of bacterial populations to natural environments, it is necessary to be able to measure the microbial community structure
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:5/1/2015)... , May 1, 2015 ... market for antithrombotic drugs will reach $22.4bn in 2019. ... Drugs: World Industry and Market Prospects 2015-2025 ... of that investigation is to provide forecasts and qualitative ... business information publisher and consultancy in London, ...
(Date:5/1/2015)... ALBANY, N.Y. , May 1, 2015 ... today that William S. Marth , AMRI,s President ... of America Merrill Lynch 2015 Health Care Conference on ... A live audio webcast of the presentation can ... http://ir.amriglobal.com/events.cfm?CalendarID=2          The webcast will be ...
(Date:5/1/2015)... RESEARCH TRIANGLE PARK, N.C. , May 1, 2015 /PRNewswire/ ... Roger Jeffs , Ph.D., President and Co-Chief Executive Officer ... the company,s business at the Deutsche Bank 40 th ... . The presentation will take place on Wednesday, ... be accessed via a live webcast on the United Therapeutics ...
(Date:4/30/2015)... 2015  Roka Bioscience, Inc. (NASDAQ: ROKA ), ... solutions for the detection of foodborne pathogens, announced plans ... of 2015 on May 7, 2015 after the close ... also host a conference call at 4:30 p.m. Eastern ... quarter financial results. During the conference call, material information ...
Breaking Biology Technology:Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 2Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 3Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 4Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 5AMRI to Present at the Bank of America Merrill Lynch 2015 Health Care Conference 2Roka Bioscience to Report First Quarter 2015 Financial Results On May 7, 2015 2
... - GeneNews Limited (TSX: GEN), a company ... the early detection of diseases and personalized ... Laboratories, leading providers of laboratory services, information ... specimen collection services for ColonSentry(TM), the world,s ...
... November 20 ,Debiopharm Group (Debiopharm), a ... serious medical conditions and particularly,oncology, and ... fully integrated, global biotech focused on ... development from target identification and,validation to ...
... Discovery S.A., a Swiss biotech firm and Laborium Biopharma, a subsidiary ... development and optimization of protein bioprocess production and scale-up. , ... ... S.A., a Swiss biotech firm and Laborium Biopharma, a subsidiary of ...
Cached Biology Technology:GeneNews rolls out ColonSentry(TM) test in additional Toronto area labs 2GeneNews rolls out ColonSentry(TM) test in additional Toronto area labs 3GeneNews rolls out ColonSentry(TM) test in additional Toronto area labs 4Debiopharm and Aurigene Sign Licensing Agreement for the Development and Commercialisation of Debio 0617, a Novel Inhibitor of an Undisclosed Oncology Pathway 2Debiopharm and Aurigene Sign Licensing Agreement for the Development and Commercialisation of Debio 0617, a Novel Inhibitor of an Undisclosed Oncology Pathway 3Med Discovery and Laborium Biopharma Sign a Bioprocess Development Partnership Agreement 2
(Date:3/23/2015)... 2015 SoundView Technology Group issues a new research ... ) Wocket smart wallet. SoundView was one of the selected user ... with the Wocket in multiple scenarios and outlets. ... other retailers, making both debit and credit card payments.  ... "If the company meets their plans in 2015, it would ...
(Date:3/23/2015)... OXFORD, Conn. , Mar. 23, 2015 NXT-ID, Inc. ... authentication company focused on the growing mobile commerce market, announces ... a new advertising campaign on CNBC television starting March 30 ... ads will commence airing in New York ... Executive Officer said: "We are excited about our new ad ...
(Date:3/20/2015)... Conn. , March 20, 2015 NXT-ID, ... a biometric authentication company focused on the growing mobile ... smart wallet is one of the products featured in ... March 20th at 11:00 PM EST on the DIY ... to Las Vegas , site of ...
Breaking Biology News(10 mins):Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 3Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 4NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 2NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 3
... Patients with symptomatic moderate asthma who were treated ... antibody, experienced significantly fewer disease exacerbations than individuals ... the first issue for October 2006 of the ... published by the American Thoracic Society. , Trevor ...
... harnessing of 'T-rays' - electromagnetic terahertz waves - which ... varied as biological cell abnormalities and explosives has been announced. ... in Spain have said they have found a way to ... Their findings are set out in a letter published in ...
... theories regarding consciousness, emotion and quality of life ... (ALS, also known as 'Lou Gerhig's disease') are ... interaction. ALS is a progressive disease that destroys ... latest issue of Psychophysiology. The article reviews the ...
Cached Biology News:Monoclonal antibody reduces exacerbations in asthmatics 2T-ray breakthrough could make detecting disease far easier 2Computer with brain connections changing quality of life of paralyzed 2
... fixative useful for routine procedures. Hollandes contains ... and will decalcify small bone specimens. Tissue ... stained successfully with most stains, and the ... blood cell membranes and cosinophil and endocrine ...
... gold standard for automated bioresearch: Developed ... applications • Based on decades of ... accessories designed for bioresearch • Holographic ... Versatility, reliability and ease-of-use • Pulsed ...
... BODIPY FL ATP-Gamma-S binds to ATP-binding ... the BODIPY FL fluorophore was linked ... addition to its potential use for ... thioether is an important new substrate ...
... the patent-pending Formula Predictor software takes ... isotope pattern verification, and unique fragment-ion ... correct formula for unknown components. ... formulae from mass spec data often ...
Biology Products: