Navigation Links
Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

, NJ), 5 M bovine serum albumin, 200 M of each primer, 4 mm MgCl2, 1 l (60100 ng) template DNA, and 10 units of Stoffel Fragment Taq polymerase (Perkin Elmer).

Site-specific genetic profiles were produced by analyzing PCR products with DGGE in the manner described by Muyzer et al.1 Six percent acrylamide stock solutions were prepared containing 0 and 100% denaturant. The 0% denaturant stock was prepared by adding 15 ml of 40% acrylamide (Fisher Scientific) plus 2 ml of 50x Tris-Acetic acid-EDTA (TAE) buffer to 83 ml of distilled water. The 100% denaturant stock solution, defined as 7 M urea and 40% formamide, was prepared by mixing 15 ml of 40% acrylamide (Fisher Scientific) with 2 ml of 50x TAE buffer with 40 ml of molecular biology grade deionized formamide (Sigma Chemical) and 42 g of molecular biology grade urea (Sigma Chemical, St. Louis, MO). Stock solutions were degassed under vacuum for 510 minutes and filtered through 0.45 m filters. Parallel-style gels were cast using the Model 475 Gradient Former (Bio-Rad, Hercules, CA). Gels were run in a DCode unit in 1x TAE at 60 C for 912 h at 170 V (Bio-Rad Model 200/2.0 Power Supply). Gels were subsequently stained with a 1:10,000 dilution of Syber Green I (Molecular Probes, Eugene, OR). Gels were photographed in a dark room with a Nikon 35 mm camera and Ilford 400 black and white film and the film negatives were digitized with a Hamamatsu CCD camera (Hamamatsu, Japan). Image analysis of DGGE gels was performed using quantitative analysis software and lane intensity profiles measured by optical density highlighted the position of individual bands. Numerical representations of the profiles were exported to Microsoft Excel spreadsheet s
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:8/19/2014)... 2014 Cellgen Diagnostics ... to fund a corporate lab for its genetic-based ... development is a critical component in the move ... the implementation of personalized medicine – a more ... , Indiegogo contributions will support Cellgen’s intent to ...
(Date:8/19/2014)... , Aug. 19, 2014 CSSi, the leader ... recently announced the formation of the company,s Medical and ... the addition of Dr. William E. Gannon, Jr. ... http://photos.prnewswire.com/prnh/20140818/136957 The MCAB, with Dr. ... of therapeutic areas and set strategic goals for the ...
(Date:8/18/2014)... 2014 Deep Research Report on ... and in-depth research report on the China Linalool ... including its definition, classification, application, and industry chain ... covers the international market analysis, including China’s domestic ... macroeconomic environment & economic situation analysis. The report ...
(Date:8/18/2014)... UPTON, NYElectronic devices with unprecedented efficiency and ... materials that use built-in electric polarizations to ... magnets inside most popular data-driven technology. But ... stumbling blocks, including a curious habit of ... at the U.S. Department of Energy,s Brookhaven ...
Breaking Biology Technology:Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 3CSSi Appoints Dr. William E. Gannon, Jr. to Newly Formed Medical and Clinical Advisory Board 2CSSi Appoints Dr. William E. Gannon, Jr. to Newly Formed Medical and Clinical Advisory Board 3Linalool Industry & Diphenylamine Market (Global, China) 2014 Analysis Now Available at DeepResearchReports.com 2Linalool Industry & Diphenylamine Market (Global, China) 2014 Analysis Now Available at DeepResearchReports.com 3Linalool Industry & Diphenylamine Market (Global, China) 2014 Analysis Now Available at DeepResearchReports.com 4Promising ferroelectric materials suffer from unexpected electric polarizations 2Promising ferroelectric materials suffer from unexpected electric polarizations 3Promising ferroelectric materials suffer from unexpected electric polarizations 4
... Mass., Nov. 3 RainDance Technologies will,present ... at the American Society of Human Genetics ... November 13th., Recent advances in DNA ... cost. However, even with the improved efficiency,of ...
... N.J., Nov. 3 Arno Therapeutics,Inc. (OTC Bulletin Board: ... announced that Dr. Roger G. Berlin,Chief Executive Officer of ... Healthcare Conference on Monday, November 10, 2008 at 2:00 ... York Palace Hotel in New York City. Dr.,Berlin will ...
... CAMBRIDGE, Mass., Nov. 3 Verenium Corporation,(Nasdaq: ... next-generation cellulosic,ethanol and high-performance specialty enzymes, announced today ... on Monday, November 10,2008 after market close. In ... conference call with live webcast on Monday, November ...
Cached Biology Technology:RainDance Technologies to Present 'Sequence Enrichment Using Droplet-Based Microfluidics' Workshop at ASHG 2008 2Arno Therapeutics to Present at the Rodman & Renshaw 10th Annual Healthcare Conference 2Verenium Corporation to Announce Third Quarter 2008 Financial Results 2Verenium Corporation to Announce Third Quarter 2008 Financial Results 3
(Date:8/20/2014)... When are athletes who have suffered concussions ready to ... has found that high school athletes who head back ... experience a significant regression in their abilities to simultaneously ... as seen in changes in their balance and/or altered ... Ten of the 12 had returned to activity in ...
(Date:8/20/2014)... rugs and plastic bags could one day be made ... petroleum, scientists are now reporting. The novel process they ... deal with its agricultural and plastic waste problems, appear ... Athanassiou, Ilker S. Bayer and colleagues at the Italian ... constantly growing. In 2012, its production reached 288 million ...
(Date:8/20/2014)... Carolina State University researchers have developed methods for electronically ... the electrical signals moths use to control those muscles. ... remotely-controlled moths, or "biobots," for use in emergency response. ... whether we can control the movement of moths for ... says Dr. Alper Bozkurt, an assistant professor of electrical ...
Breaking Biology News(10 mins):Recovery reversal seen in Oregon study of returning concussed athletes 2Recovery reversal seen in Oregon study of returning concussed athletes 3Research paves way for development of cyborg moth 'biobots' 2
... CREEK, Calif. More than a thousand microbial genomes have ... 15 years to better understand their roles in tasks ... estimates suggest roughly 10,000 microbial genomes will be publicly ... have not caught up with the technological advances that ...
... LANSING, Mich. A Michigan State University researcher is ... leg of the Transamazon Highway a 700-mile dirt ... ends in the Brazilian Amazon. This summer,s trip ... the National Science Foundation, into the impact of tree ...
... of the volcano in Iceland has drawn attention to ... and travelers remained stranded for days to weeks, as ... closures of major airports. The flow of particles, ... to work done by Virginia Tech,s Shane Ross of ...
Cached Biology News:DOE JGI produces new QC tool for microbial genomes 2DOE JGI produces new QC tool for microbial genomes 3DOE JGI produces new QC tool for microbial genomes 4MSU environmental scholar heads into heart of the Amazon 2Pollution dispersion research aids understanding of 2002 break-up of Antarctic ozone hole 2Pollution dispersion research aids understanding of 2002 break-up of Antarctic ozone hole 3
Mouse monoclonal [11C9] to Zeralenone ( Abpromise for all tested applications). Antigen: Chemical / Small Molecule conjugated to BSA: Zeralenone...
Anti-human C5a/C5a des-Arg (neo-epitode), Clone 2952, Monoclonal Antibody...
Reacts with the 80 kDa fragment of the M-chain of human merosin....
ICAD / DFF45 (NT) Affinity Purified Polyclonal Antibody...
Biology Products: