Analysis Of Chloramphenicol by Negative Ion Electrospray LC/MS/MS ( hod used a 6 minute run cycle with CAP ...)

Analysis Of Chloramphenicol by Negative Ion Electrospray LC/MS/MS

hod used a 6 minute run cycle with CAP giving a retention time of 3.1 minutes with a peak width at half height of 0.12 minutes. Both product ions can be quantitatively analyzed at the level of 100 fg/ μL in solution (Figure 1). The linearity of the detector response was found to be excellent (Figure 2). The R_²for 321>152 and 321>257 are 0.999 and 0.998, respectively. In addition, good precision was found at these low levels with RSD (relative standard deviations) of ~6% (Figure 3).

The limit of detection (LOD) is estimated to be <30 fg/μL in solution corresponding to a LOD of <3 pg/g in shrimp according to the U.S. FDA method.

Conclusion

The choice of either product ion for quantitation is especially important when complex matrices interfere with one of the product ions. The Varian 1200L LC/MS/MS system demonstrates excellent sensitivity, reproducibility and performance for the analyses of chloramphenicol and is able to assist in the detection of potentially harmful substance in food imports/ exports to improve food safety throughout the world.

Reference

1. B.K. Neuhaus, J.A. Hurlbut. and W. Hammack LC/MS/MS Analysis of Chloramphenicol in Shrimp. U.S. FDA Laboratory Information Bulletin No. 4290, Volume 18, No. 9, September 2002 .
http://vm.cfsan.fda.gov/~frf/lib4290.html

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( hod used a 6 minute run cycle with CAP ...)Analysis Of Chloramphenicol by Negative Ion Electrospray LC/MS/MS