Navigation Links
Analysis Of Chloramphenicol by Negative Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc.

Introduction

Chloramphenicol (CAP) is a potent, broad-spectrum antibiotic and potential carcinogen used in humans only at therapeutic doses for treatment of serious infections. Its use in meatproducing animals, food producing insects, aquaculture, and animal-feed products has been banned in the United States, Canada and the European Union. However, the illegal use of CAP remains a possibility due to its broad activity, ready availability, and low cost.

CAP can cause an irreversible illness called aplastic anemia. The incidence rate of aplastic anemia is one out of every 25,000 to 40,000 people. Onset may occur weeks or months after ending treatment with CAP. A very low concentration of CAP may be enough to trigger the illness and the safe level of dosage has not been identified.

Due to known effects of CAP and the recent discovery of CAP in imported food, the U.S. Food and Drug Administration (FDA) and other government agencies throughout the world have increased sampling and surveillance of imported shrimp, crawfish, honey, royal jelly, feed, and milk products for the presence CAP. In addition, the U.S. FDA has developed an LC/MS/MS method to determine CAP in shrimp.1 The detection limit for the method is 80 pg/g* of shrimp, corresponding to 800 fg/μL of the solution injected into the LC/MS.

Instrumentation

Varian ProStar 430 AutoSampler

Varian ProStar 210 Isocratic Solvent Delivery Modules

Varian 1200L LC/MS equipped with ESI source

Materials and Reagents

Chloramphenicol (Part No. C1919) from Sigma-Aldrich Corp. (St. Louis, Missouri, USA).

All other chemicals are reagent grade or HPLC grade.

Results and Discussion

The LC method used a 6 minute run cycle with CAP giving a retention time of 3.1 minutes with a peak width at half height of 0.12 minutes. Both product ions can be quantitatively analyzed at the level of 100 fg/ μL in solution (Figure 1). The linearity of the detector response was found to be excellent (Figure 2). The R2 for 321>152 and 321>257 are 0.999 and 0.998, respectively. In addition, good precision was found at these low levels with RSD (relative standard deviations) of ~6% (Figure 3).

The limit of detection (LOD) is estimated to be <30 fg/μL in solution corresponding to a LOD of <3 pg/g in shrimp according to the U.S. FDA method.

Conclusion

The choice of either product ion for quantitation is especially important when complex matrices interfere with one of the product ions. The Varian 1200L LC/MS/MS system demonstrates excellent sensitivity, reproducibility and performance for the analyses of chloramphenicol and is able to assist in the detection of potentially harmful substance in food imports/ exports to improve food safety throughout the world.

Reference

1. B.K. Neuhaus, J.A. Hurlbut. and W. Hammack LC/MS/MS Analysis of Chloramphenicol in Shrimp. U.S. FDA Laboratory Information Bulletin No. 4290, Volume 18, No. 9, September 2002 .
http://vm.cfsan.fda.gov/~frf/lib4290.html


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... Diego, CA (PRWEB) , ... June 27, 2016 , ... ... solutions for clinical trials, announced today the Clinical Reach Virtual Patient Encounter ... their care circle with the physician and clinical trial team. , Using the CONSULT ...
(Date:6/27/2016)... 27, 2016  Liquid Biotech USA ... of a Sponsored Research Agreement with The University ... (CTCs) from cancer patients.  The funding will be ... correlate with clinical outcomes in cancer patients undergoing ... then be employed to support the design of ...
(Date:6/24/2016)... 24, 2016 Epic Sciences unveiled a ... susceptible to PARP inhibitors by targeting homologous recombination ... The new test has already been incorporated into ... cancer types. Over 230 clinical trials ... pathways, including PARP, ATM, ATR, DNA-PK and WEE-1. ...
(Date:6/23/2016)... ... June 23, 2016 , ... Mosio, a ... eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with experienced clinical research ... by providing practical tips, tools, and strategies for clinical researchers. , “The landscape ...
Breaking Biology Technology:
(Date:5/12/2016)... WearablesResearch.com , a brand of Troubadour Research & ... Q1 wave of its quarterly wearables survey. A particular ... a program where they would receive discounts for sharing ... "We were surprised to see that so many ... CEO of Troubadour Research, "primarily because there are segments ...
(Date:4/26/2016)... and LONDON , April ... part of EdgeVerve Systems, a product subsidiary of ... today announced a partnership to integrate the Onegini ...      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) ... their customers enhanced security to access and transact ...
(Date:4/14/2016)... Israel , April 14, 2016 ... Authentication and Malware Detection, today announced the appointment of ... assumed the new role. Goldwerger,s leadership appointment ... on the heels of the deployment of its platform ... BioCatch,s behavioral biometric technology, which discerns unique cognitive and ...
Breaking Biology News(10 mins):