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An Optimal DNA Microarray Substrate for the Identification of Fetal ,,, and Somatic Liver Stem Cells of the Rat

liver metabolism. The biological function of these reactions is the transformation of toxic substances or external materials and thereby the reduction of their biological activity, as well as the increase of their polarity. The Cytochrome P 450 system is mainly involved in this process. In phase II, various substrates are coupled to very polarized, negatively charged molecules. The resulting conjugates are considerably more water soluble in comparison to unconjugated compounds, and thereby are easier to eliminate. The Glutathione S-Transferase, for example, belongs to the family of phase II enzymes. Fetal hepatocytes can be stimulated to increased proliferation by the creation of optimal culture conditions. The initial cell number can be increased up to 30 times, depending upon the combination of growth factors. With the microarrays specially developed for this process, it was demonstrated that the liver-specific functions (phase I and phase II enzymes) are expressed for up to 10 days in culture under optimal conditions.


Fig. 4: Optimal signal to background ratio
Hybridization of a Rat Liver Chip on CreativeChip 3D Slide with Cy3-labeled cDNA from 100 g of rat liver RNA

Fig. 5: Detail of a hybridized Rat Liver Chip, showing the consistency of the replicates
Conclusion Evaluation of the experimental system

The CreativeChip 3D Slides are characterized by a constantly low intrinsic fluorescence so the dynamic range of measurable signals is very high. Binding the probes (in this case: 50mer oligonucleotides)
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