Navigation Links
An Optimal DNA Microarray Substrate for the Identification of Fetal ,,, and Somatic Liver Stem Cells of the Rat

liver metabolism. The biological function of these reactions is the transformation of toxic substances or external materials and thereby the reduction of their biological activity, as well as the increase of their polarity. The Cytochrome P 450 system is mainly involved in this process. In phase II, various substrates are coupled to very polarized, negatively charged molecules. The resulting conjugates are considerably more water soluble in comparison to unconjugated compounds, and thereby are easier to eliminate. The Glutathione S-Transferase, for example, belongs to the family of phase II enzymes. Fetal hepatocytes can be stimulated to increased proliferation by the creation of optimal culture conditions. The initial cell number can be increased up to 30 times, depending upon the combination of growth factors. With the microarrays specially developed for this process, it was demonstrated that the liver-specific functions (phase I and phase II enzymes) are expressed for up to 10 days in culture under optimal conditions.


Fig. 4: Optimal signal to background ratio
Hybridization of a Rat Liver Chip on CreativeChip 3D Slide with Cy3-labeled cDNA from 100 g of rat liver RNA

Fig. 5: Detail of a hybridized Rat Liver Chip, showing the consistency of the replicates
Conclusion Evaluation of the experimental system

The CreativeChip 3D Slides are characterized by a constantly low intrinsic fluorescence so the dynamic range of measurable signals is very high. Binding the probes (in this case: 50mer oligonucleotides)
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
2. Select the Optimal Enzyme for RT-PCR
3. Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B
4. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
5. New Microarray Labeling Kit for Preparing cDNA Probes
6. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
7. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
10. Increased Sensitivity in Microarray Analysis
11. Analysis of Microarray Data
Post Your Comments:
(Date:5/28/2015)... (PRWEB) May 28, 2015 Global ... a point to ask his audiences if they ... trends including robotics, solar cells, fuel cells, rapid ... printers, advanced battery storage technology, driverless cars, artificial ... high-quality but inexpensive online education. , While ...
(Date:5/28/2015)... In two different WPC forums , Asymmetrex’s ... present the company’s unique approach to developing technologies for ... will describe Asymmetrex ’s innovation in integrating its ... produce the first-ever technology for quantitative monitoring of adult ... culture. When leading an interactive breakout discussion group, ...
(Date:5/27/2015)... 27, 2015 RXi Pharmaceuticals Corporation (NASDAQ: ... discovering and developing innovative therapies primarily in the ... has commenced a public offering of shares of ... its common stock and overallotment purchase rights to ... of common stock, warrants and overallotment purchase rights ...
(Date:5/27/2015)... The Academy of Model Aeronautics Foundation would ... Model Aviation Day. The third annual celebration will be August ... the model aviation hobby nation-wide and to introduce model flying ... 2,350 chartered clubs to celebrate the hobby and share it ... There will also be a local event at the AMA ...
Breaking Biology Technology:Futurist Jack Uldrich to Deliver 4 Keynote Addresses in June 2Asymmetrex Will Discuss the Importance of Adult Tissue Stem Cell Time with 3D Tissue Engineers at the 14th Annual World Preclinical Congress in Boston in June 2Asymmetrex Will Discuss the Importance of Adult Tissue Stem Cell Time with 3D Tissue Engineers at the 14th Annual World Preclinical Congress in Boston in June 3RXi Pharmaceuticals Announces Proposed Public Offering of Common Stock and Warrants 2RXi Pharmaceuticals Announces Proposed Public Offering of Common Stock and Warrants 3Academy of Model Aeronautics Foundation to host National Model Aviation Day Motorcycle Rally and Family Fest in Muncie, IN 2Academy of Model Aeronautics Foundation to host National Model Aviation Day Motorcycle Rally and Family Fest in Muncie, IN 3
... SiliconSky GPS, designs and develops a GPS-enabled asthma inhaler that will ... , ... Red Lodge, MT (PRWEB) April 22, 2009 ... product design firm, announced today that is has successfully completed the ...
... WASHINGTON, April 21 The Pharmaceutical Research and Manufacturers ... Families USA today unveiled a campaign to promote three ... coverage for all Americans. PhRMA and Families USA will ... to ensure that these policies are incorporated in the ...
... and Tracking ToolJERICHO, N.Y., April 21 HCCS (Health ... healthcare compliance and competency solutions, today announced the release ... real-time tracking of Conflict of Interest disclosures. HCCS partnered ... this comprehensive tool in response to tighter regulations, including ...
Cached Biology Technology:SiliconSky GPS Designs First AGPS-Enabled Asthma Inhaler for Landmark Research Program 2SiliconSky GPS Designs First AGPS-Enabled Asthma Inhaler for Landmark Research Program 3PhRMA, Families USA Jointly Promote Measures to Ensure Quality, Affordable Healthcare Coverage 2PhRMA, Families USA Jointly Promote Measures to Ensure Quality, Affordable Healthcare Coverage 3Managing Conflict of Interest Disclosures Simplified for Healthcare Organizations 2
(Date:5/20/2015)... 2015 Research ... the addition of the  "5-year Opportunity ... Market"  report to their offering.  ,     ... analyses trends in the iris recognition ... sectors, globally. Despite hardware pricing and ...
(Date:5/19/2015)... , May 18, 2015 Fingerprint ... sensors FPC1020, FPC1025, FPC1145, FPC1155 and FPC1035 from one if ... June until and including Q3 2015 and the sensors will ... . Communicated order values for 2015 hereby amount to 740 ... Q1 2015 of 140 MSEK and a number of smaller ...
(Date:5/19/2015)... , May 19, 2015 ... has announced the addition of the  "Genetic ... offering.  ,     (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , ,A recent ... an in-depth analysis of the current and ... of gene-based tests, their working principles and ...
Breaking Biology News(10 mins):Global Iris Recognition Market Report 2015 - 5-Year Opportunity Analysis 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Global Genetic Testing Market Outlook 2018 2
... forms far deeper than geologists previously thought, according to new ... of Rice University put very small samples of peridotite, rock ... The scientists found that the rock can and does ... those found as deep as 250 kilometers down in the ...
... conditions lead to what kinds of bacterial infections? If doctors ... pre-treatment with certain kinds of antibiotics. A University of Colorado ... International Journal of Infectious Diseases shows the answer: ... especially prevalent in patients with lung and GI cancers, more ...
... Kan. -- A competitive grant is helping a Kansas ... spreading one of the worst plant diseases into a ... Badillo-Vargas, a plant pathology doctoral student, Puerto Rico, recently ... $71,000 from the U.S. Department of Agriculture,s National Institute ...
Cached Biology News:Magma in Earth's mantle forms deeper than once thought 2Magma in Earth's mantle forms deeper than once thought 3Planning for bacteria in cancer patients may help hospitals fight infections 2USDA grant advancing deadly plant disease, insect research 2
... fast and reliable scalable automation for today's drug discovery ... are available in three base configurations; Mini Workstation Series, ... ... All Staccato Application Series systems use a ...
... provide fast and reliable automation for ... drug development laboratories. Staccato Custom Series ... providing solutions that are tailored to ... Systems are controlled with either Caliper's ...
... in any standard fixed-angle microcentrifuge rotor ... g.,Spin- centrifuge tube filters consist of ... centrifuge tube. They filter by centrifugation ... sample preparation, removal of cells from ...
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. Material: polystyrene ...
Biology Products: