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An Optimal DNA Microarray Substrate for the Identification of Fetal ,,, and Somatic Liver Stem Cells of the Rat

cultivated on collagen 1 w/ HGF Fig. 2: Hybridization of a Rat Liver Chip printed on Eppendorf CreativeChip 3D Slide
Cy3 labeled cDNA: synthesized from 100 g total RNA from hepatocytes cultivated on collagen 1 w/o HGF
Cy5 labeled cDNA: synthesized from 100 g total RNA from hepatocytes cultivated on collagen 1 w/ HGF Figures 1 and 2 show a Colorswitch experiment in which the direct influence of HGF on the expression level of liver-specific genes is analyzed.

To do this, fetal hepatocytes were cultivated under 2 different culture conditions (collagen 1 without HGF and collagen 1 with HGF, respectively). The expression of liver-specific markers, such as albumin, Cytochrome P 450, GST, etc., in the various cultivated hepatocytes was subsequently tested with the Rat Liver Chip.

The culture condition 2 (collagen 1 with HGF) led to an up-regulation of liver-specific genes in both experiments.

Fig. 3: Two-dimensional scatter plot analysis (Cy3 against Cy5 fluorescence signal, logarithmic) of a typical experiment according to Figure 1.
The diagonal through the origin represents the anticipated AE of 1. As a result, HGF leads to an up-regulation of liver-specific genes. Results

The developed Rat Liver Chip includes 240 liver-specific genes, which primarily encode phase I and phase II enzymes and matrices. Oxidation, reduction, methylation and desulphurizing reactions, as well as hydrolytic cleavages occur in phase I of the
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