Navigation Links
An Optimal DNA Microarray Substrate for the Identification of Fetal ,,, and Somatic Liver Stem Cells of the Rat

cultivated on collagen 1 w/ HGF Fig. 2: Hybridization of a Rat Liver Chip printed on Eppendorf CreativeChip 3D Slide
Cy3 labeled cDNA: synthesized from 100 g total RNA from hepatocytes cultivated on collagen 1 w/o HGF
Cy5 labeled cDNA: synthesized from 100 g total RNA from hepatocytes cultivated on collagen 1 w/ HGF Figures 1 and 2 show a Colorswitch experiment in which the direct influence of HGF on the expression level of liver-specific genes is analyzed.

To do this, fetal hepatocytes were cultivated under 2 different culture conditions (collagen 1 without HGF and collagen 1 with HGF, respectively). The expression of liver-specific markers, such as albumin, Cytochrome P 450, GST, etc., in the various cultivated hepatocytes was subsequently tested with the Rat Liver Chip.

The culture condition 2 (collagen 1 with HGF) led to an up-regulation of liver-specific genes in both experiments.

Fig. 3: Two-dimensional scatter plot analysis (Cy3 against Cy5 fluorescence signal, logarithmic) of a typical experiment according to Figure 1.
The diagonal through the origin represents the anticipated AE of 1. As a result, HGF leads to an up-regulation of liver-specific genes. Results

The developed Rat Liver Chip includes 240 liver-specific genes, which primarily encode phase I and phase II enzymes and matrices. Oxidation, reduction, methylation and desulphurizing reactions, as well as hydrolytic cleavages occur in phase I of the
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
2. Select the Optimal Enzyme for RT-PCR
3. Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B
4. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
5. New Microarray Labeling Kit for Preparing cDNA Probes
6. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
7. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
10. Increased Sensitivity in Microarray Analysis
11. Analysis of Microarray Data
Post Your Comments:
(Date:12/19/2014)... LayerBio is an MIT spin-off developing novel drug ... Eye Institute (NEI) at the National Institutes of Health ... to develop a drug-eluting intraocular lens (IOL) for cataract ... vision loss in people over age 40 and the ... Mandell, LayerBio’s Founder and CEO, "There is a critical ...
(Date:12/19/2014)... In contrast to traditional LED lights where the spectrum ... proprietary LED technology to incorporate their spectra in a ... critical in low proximity installations. The Valoya L-series offers ... which is up to four times longer than commonly ... was clearly higher when compared to traditional fluorescent or ...
(Date:12/19/2014)... MILLBROOK, N.Y. , Dec. 18, 2014 Egenix, ... has been elected Chairman of the Board, to succeed ... President and CEO, but will continue as a member of ... that he has added to the company over almost twenty ... The Board of Directors has established a Search ...
(Date:12/17/2014)... Based on the revenues gained from ... is dominated by four major players contributing to ... players include GE Healthcare (U.K.), Siemens AG (Germany), ... Full Report Copy at http://www.marketsandmarkets.com/Market-Reports/surgical-imaging-market-210534462.html , ... attributed to its strong product portfolio, which includes ...
Breaking Biology Technology:LayerBio Awarded NIH Grant to Develop a Drug Delivery Device for Cataract Surgery 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4
... in a lab dish to the steroid DHEA, exhibit a ... play a role in helping the brain produce new cells, ... online editions of the Proceedings of the National Academy of ... of scientists at the University of Wisconsin-Madison, provides some of ...
... , a data quality solutions provider, announced today ... Information Quality Suite to work with Oracle Warehouse ... intelligence integration design tool that manages data lifecycles. ... intelligence application and customize the way data quality ...
... Inc. announced Tuesday that its genetic analysis ... chromosomal analysis, opening a new market to the ... extension of the Invader technology, Madison-based Third Wave ... (ASRs) for prenatal chromosomal analysis to the University ...
Cached Biology Technology:DHEA Boosts Growth Rate of Human Neural Stem Cells 2DHEA Boosts Growth Rate of Human Neural Stem Cells 3
(Date:11/21/2014)... , Nov. 20, 2014   Atmel® Corporation ... microcontroller (MCU) and touch technology solutions, today launched the ... with the widest V cc range from ... accuracy and faster I 2 C bus communication speeds, ... EEPROM memory making them ideal for consumer, industrial, computer, ...
(Date:11/18/2014)... BETHESDA, Md. , Nov. 17, 2014 The ... European and U.S. regulatory agencies will speak and at least ... Quality Metrics Conference at the Omni Shoreham Hotel in ... is pleased once again to have significant support from the ... and Europe in our effort to ...
(Date:11/12/2014)... Nov. 12, 2014 Crossmatch™, a leading provider ... U.are.U ® fingerprint readers have been deployed throughout ... Central Mexico . The bakery chain implemented ... payroll issues caused by employees clocking in for each ... fingerprint readers, Montparnasse relied on paper timecards and a ...
Breaking Biology News(10 mins):Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2
... M.D., director of the National Institute of Allergy and Infectious ... been awarded the 2007 Mary Woodard Lasker Award for Public ... health programs, in AIDS and biodefense. The award will be ... New York City. , Dr. Fauci is honored for his ...
... in conjunction with the National Evolutionary Synthesis Center, will ... 1, 2007, at the Hyatt Regency Hotel in Atlanta, ... and Populations, is part of the 2007 Professional Development ... The aim of the symposium is ...
... National Institutes of Health has awarded one of 12 ... a team based at the University of Chicago Medical ... 2006, form the core of an NIH effort to ... "transform how clinical and translational research is conducted," ultimately ...
Cached Biology News:Anthony S. Fauci awarded Lasker Award for Public Service 2Anthony S. Fauci awarded Lasker Award for Public Service 3Anthony S. Fauci awarded Lasker Award for Public Service 4AIBS to cohost symposium on evolution, disease and human health 2AIBS to cohost symposium on evolution, disease and human health 3AIBS to cohost symposium on evolution, disease and human health 4NIH awards nearly $23M to University of Chicago for translational research 2NIH awards nearly $23M to University of Chicago for translational research 3NIH awards nearly $23M to University of Chicago for translational research 4
... Agarose Gel-Digesting Preparation contains a unique β-agarose ... quantitative recovery of intact DNA and RNA ... following electrophoresis in TAE, TBE, MOPS, or ... directly in the TAE, TBE, MOPS, and ...
...
... a unique β-agarose digesting enzyme developed at ... DNA and RNA from low melting point ... TBE, MOPS, or phosphate buffers. The gel ... TBE, MOPS, and phosphate electrophoresis buffers or ...
Request Info...
Biology Products: