To do this, fetal hepatocytes were cultivated under 2 different culture conditions (collagen 1 without HGF and collagen 1 with HGF, respectively). The expression of liver-specific markers, such as albumin, Cytochrome P 450, GST, etc., in the various cultivated hepatocytes was subsequently tested with the Rat Liver Chip.
The culture condition 2 (collagen 1 with HGF) led to an up-regulation of liver-specific genes in both experiments.Fig. 3: Two-dimensional scatter plot analysis (Cy3 against Cy5 fluorescence signal, logarithmic) of a typical experiment according to Figure 1.
The developed Rat Liver Chip includes 240 liver-specific genes, which
primarily encode phase I and phase II enzymes and matrices. Oxidation,
reduction, methylation and desulphurizing reactions, as well as hydrolytic
cleavages occur in phase I of the