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An Optimal DNA Microarray Substrate for the Identification of Fetal ,,, and Somatic Liver Stem Cells of the Rat

Dr. Frank Stahl
GBF Gesellschaft fr Biotechnologische Forschung mbH
(Society for Biotechnological Research) Introduction

DNA microarrays are a key technology in modern molecular biotechnology. Due to their high degree of parallelism, they allow the simultaneous analysis of complex genetic changes, thereby enabling a deeper insight into processes such as differentiation, growth and cell development.

In our project we are developing DNA microarrays for the identification of fetal and somatic liver stem cells. The microarrays will be used for the examination of the differentiation status of these cells, which is influenced by certain factors of the extra-cellular microenvironment in vitro.

The results are of great significance for an understanding of embryonic and fetal organ development. They provide the basics for both the understanding of developmental biology and for the development of toxicologically and clinically applicable organ replacement systems, which are of great importance for tissue engineering technologies.

This technology field focuses on the development of biological substituents that maintain, regenerate and improve tissue and organ functions. Excluding ethical and technical problems, modified tissue or tissue cultures will be optimized in terms of material and time for tissue and organ transplantation. In this respect, the genetic background of cell differentiation has developed into a focal point of research interest in the last few years.

Goal

In our project, fetal hepatocytes of the rat should undergo the most extensive proliferation and diff
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