Navigation Links
An Epitope Tagging Vector for Gene Expression in Mammalian Cells

of the target protein, and highly immunoreactive. The FLAG epitope is a synthetic epitope that consists of eight amino acid residues (DYKDDDDK).5 The c-myc epitope is derived from the human c-myc gene and contains 10 amino acid residues (EQKLISEEDL).6 In addition to the epitope tag sequences, the pCMV-Tag1 vector contains features for expression of fusion proteins in eukaryotic cells. The cytomegalovirus (CMV) promoter allows constitutive expression of the cloned DNA in a wide variety of mammalian cell lines. The neomycin-resistance gene is under control of both the prokaryotic b-lactamase promoter to provide kanamycin resistance in bacteria and the SV40 early promoter to provide G418 resistance in mammalian cells. The multiple cloning site (MCS) of the pCMV-Tag1 vector is arranged to allow a variety of cloning strategies to be used, resulting in C-terminal, N-terminal or internal tagging of the protein of interest. A Kozak consensus sequence of GCC(A or G)CCATGG7 provides optimal expression of the fusion protein when the N-terminal FLAG epitope tag is used. Other cloning options, which require fusion proteins to include their own translational start sequence, are also possible.

Cloning Strategies for Epitope Tagging

Epitope Tag

Tag Location

Cloning Site

Kozak Sequence
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
3. A New C-Terminal GST Vector for Protein Production in S. pombe
4. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
5. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
8. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
9. A New Lambda Vector for Mammalian Expression
10. Versatile Reporter Vectors for Monitoring Viral Transduction
11. High-Titer Retroviral Vectors for Gene Delivery
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Epitope Tagging Vector for Gene Expression Mammalian Cells

(Date:3/6/2015)... NY (PRWEB) March 06, 2015 Transparency Market ... Vegetables and Other Crops - Global Industry Analysis, Size, Share, ... report, the global isoxaflutole market was valued at US$ 116.3 ... Mn by 2020, expanding at a CAGR of 5.1% from ... for isoxaflutole stood at 2,569.5 kilo tons in 2013. , ...
(Date:3/5/2015)... 5, 2015  DuPont (NYSE: DD ) ... Industrials Conference held today in New York ... Trerotola discussed strategy, progress and plans to accelerate ...  Strengthening and growing the company,s leading position in high-value ... strategic priority for DuPont. Discussing the company,s ...
(Date:3/5/2015)... 05, 2015 Plant disease specialists from ... at the Annual Meeting of The American Phytopathological Society. ... released online now at APSnet.org/meet . , The ... meeting, to be held August 1-5, 2015 in Pasadena, ... Field Trips, Workshops, and committee meetings, all carefully balanced ...
(Date:3/5/2015)... and TORONTO , March 5, ... a clinical-stage company developing new therapeutics and molecular diagnostics ... that William G. Rice , Ph.D., Chairman, President ... 27th Annual ROTH Conference on Tuesday, March 10 th ... Carlton, Laguna Niguel, CA. Dr. ...
Breaking Biology Technology:Isoxaflutole Market is Expected to Reach US$ 163.0 Mn by 2020 : Transparency Market Research 2Isoxaflutole Market is Expected to Reach US$ 163.0 Mn by 2020 : Transparency Market Research 3DuPont Leader Highlights Strategy, Progress and Plans to Accelerate Growth in Advanced Materials and Discusses Near-Term Outlook 2DuPont Leader Highlights Strategy, Progress and Plans to Accelerate Growth in Advanced Materials and Discusses Near-Term Outlook 3DuPont Leader Highlights Strategy, Progress and Plans to Accelerate Growth in Advanced Materials and Discusses Near-Term Outlook 4American Phytopathological Society Announces Annual Meeting Daily Schedule 2Aptose Biosciences to Present at the 27th Annual ROTH Conference 2Aptose Biosciences to Present at the 27th Annual ROTH Conference 3
... DUBLIN, Ireland, January 9 Azur Pharma ... the exclusive U.S. marketing rights,for Niravam(R) (alprazolam ... Kemstro(TM) (baclofen,orally disintegrating tablets) and Fluxid(TM) (famotidine ... terms were not,disclosed. , ...
... The IN VIVO Blog , which provides news, insights and ... a Deal of the Year for 2008 -- ... than 2,500 reader votes were cast in this inaugural competition, ... the 13 nominees selected by The IN VIVO Blog,s writers ...
... MATK ) announced today that it will be ... The conference is being held January 14-15, 2009 at ... Point, California. Peter L. Buzy, Chief Financial Officer, and ... 2009 at 1:25 PM PT.A live audio webcast of Martek,s ...
Cached Biology Technology:Azur Pharma Announces the Licensing of Niravam, Parcopa, Kemstro and Fluxid From UCB 2The IN VIVO Blog Selects First Annual 'Deal of the Year' 2
(Date:2/5/2015)... New Market Research Reports Title ... To 2020 has Been Added to GrandViewReseach.com Report Database ... to reach USD 5.10 billion by 2020, according to ... cameras help identify the site of tissue damage in ... demand in medical imaging applications. They have been extensively ...
(Date:2/5/2015)... , Feb. 3, 2015 Despite a ... from industries such as consumer electronics, automotive, storage ... surface mount technology (SMT) screen printers. Innovations in ... control will push the adoption curve up. Meanwhile, ... steady as glue dispensers are indispensable in the ...
(Date:1/22/2015)... 13, 2015  Today, FreeWavz ( www.FreeWavz.com ), the ... campaign on Fundable, https://www.fundable.com/freewavz . FreeWavz will ... capacity to meet customer demand. Logo ... http://photos.prnewswire.com/prnh/20150113/168790 Invented by Dr. ...
Breaking Biology News(10 mins):IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 3End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 2End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 3FreeWavz Launches on Fundable to Drive Speed-to-Market in 2015 2
... The Netherlands, December 15 WCC Services, the,leading ... announced that,version 6 of its flagship product, ELISE, ... may receive hundreds of applicants for,each job opening ... for the job.,Security concerns are also at peak ...
... Society for Microbiology (ASM) has been honored with non-profit ... its public outreach initiatives. The awards were announced ... Society,s MicrobeWorld Video series received the award for best ... series strives to increase public understanding and appreciation of ...
... GeneVision: the uncanny ability to view the activity of ... modified camera. With GeneVision, military commanders could compare ... track genes related to craving as shoppers moved about ... secret advantage over his love-struck dates. Frightening? Perhaps. ...
Cached Biology News:WCC Announces Version 6 of its ELISE Smart Search and Match Platform for Employment and Biometric Matching 2Real-time gene monitoring developed 2
... is formulated for covering tissue sections and cell ... OR alcohol insoluble end products. UltraMount ... Fast Red or AEC that require an aqueous ... as DAB. No heating is required prior to ...
... Rabbit polyclonal to JAK2 (phospho ... tested applications). Antigen: ... JAK2 around the phosphorylation site of ... Entrez Gene ID: ...
Rabbit polyclonal to JNK1 (phospho T183 + Y185) ( Abpromise for all tested applications). entrezGeneID: 5599 SwissProtID: P45983...
... ( Abpromise for all tested applications). ... conjugated to KLH derived from within residues ... amino acid sequence is proprietary)(Peptide available as ... Gene ID: 55693 ...
Biology Products: