( An Epitope Tagging Vector for Gene Exp...)An Epitope Tagging Vector for Gene Expression in Mammalian Cells

Tanya Hosfield Quinn Lu
Stratagene

The pCMV-Tag1 vector is an epitope tagging vector designed for gene expression in mammalian cells. A target gene inserted into the pCMV-Tag1 vector can be tagged with the FLAG epitope (N-terminal, C-terminal or internal tagging), the c-myc epitope (C-terminal) or both the FLAG (N-terminal) and c-myc (C-terminal) epitopes. Tagged constructs generated in the pCMV-Tag1 vector can be transfected into mammalian cells, and the tagged gene product can be easily characterized using commercially available, tag-specific antibodies.

The epitope tagging technique involves fusion of a protein of interest to a peptide-epitope that is recognized by a readily available antibody. In this technique, expression of the fusion protein is monitored using a tag-specific antibody, allowing a new protein to be studied without generating a new, specific antibody to that protein. Epitope tagging can be used to localize gene products in living cells, identify associated proteins, track movement of fusion proteins within the cell, or characterize new proteins by immunoprecipitation.^1-3

pCMV-Tag1 Vector for Epitope Tagging

Stratagenes pCMV-Tag1 vector is a general mammalian expression vector that contains the FLAG and c-myc epitopes positioned for either terminal or internal tagging of a target protein. The pCMV-Tag1 vector (figure 1) is derived from the pCMV-Script vector⁴ and contains sequences for the FLAG and c-myc epitopes. These specific epitope tags are small, not interfering with the function
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