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An Easy-to-Use Research Fermentor/Bioreactor

Designed for Culturing Mammalian, Insect, Plant, Bacteria, and Yeast Cells


Rajul Vyas, Product Manager
New Brunswick Scientific Co. Inc, 44 Talmadge Road, Edison, NJ 08817;
1-800-631-5417 or 1-732-287-1200; bioinfo@nbsc.com, www.nbsc.com/bi5.htm


Although shake flasks, T-flasks, or roller bottles have become the lab standard in most fermentation and cell culture research labs, lack of controls for pH and dissolved oxygen (DO) cause an inherent limitation in cell yields and protein expression. When greater yields are needed, a single fermentor or bioreactor can be used to increase yields by 10-fold or more, with the added advantage that it eliminates the requirement for added bench/floor space and labor.

New Brunswick Scientifics BioFlo 110 fermentor/bioreactor is a simple, versatile tool for producing biomass or secreted products from a wide variety of suspension or anchoragedependent cell lines. In this report, well show how it was used to produce high cell density yields from a variety of bacterial, mammalian, insect, and yeast cell lines with only a simple addition of control modules or vessel substitution. Complete materials and methods are documented on our website at www.nbsc.com/bi5.htm

It should be noted that in our studies we did not attempt to optimize process parameters, feeding schedules or yields; so these results, or better, are typical of what might be expected using this highly versatile fermentor/bioreactor in your laboratory.


ABOUT THE BIOFLO 110
BioFlo 110 is a research-scale fermentor/bioreactor, capable of controlling one to four vessels simultaneously. A modular design allows researchers complete flexib
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