Navigation Links
An Easy-to-Use Research Fermentor/Bioreactor

Designed for Culturing Mammalian, Insect, Plant, Bacteria, and Yeast Cells


Rajul Vyas, Product Manager

New Brunswick Scientific Co. Inc, 44 Talmadge Road, Edison, NJ 08817;
1-800-631-5417 or 1-732-287-1200; bioinfo@nbsc.com, www.nbsc.com/bi5.htm


Although shake flasks, T-flasks, or roller bottles have become the lab standard in most fermentation and cell culture research labs, lack of controls for pH and dissolved oxygen (DO) cause an inherent limitation in cell yields and protein expression. When greater yields are needed, a single fermentor or bioreactor can be used to increase yields by 10-fold or more, with the added advantage that it eliminates the requirement for added bench/floor space and labor.

New Brunswick Scientifics BioFlo 110 fermentor/bioreactor is a simple, versatile tool for producing biomass or secreted products from a wide variety of suspension or anchoragedependent cell lines. In this report, well show how it was used to produce high cell density yields from a variety of bacterial, mammalian, insect, and yeast cell lines with only a simple addition of control modules or vessel substitution. Complete materials and methods are documented on our website at www.nbsc.com/bi5.htm

It should be noted that in our studies we did not attempt to optimize process parameters, feeding schedules or yields; so these results, or better, are typical of what might be expected using this highly versatile fermentor/bioreactor in your laboratory.


ABOUT THE BIOFLO 110
BioFlo 110 is a research-scale fermentor/bioreactor, capable of controlling one to four vessels simultaneously. A modular design allows researchers complete flexibility to order the individual components they need now and add supplemental control modules and vessels at any time, should process requirements change. To simplify ordering, prepackaged fermentation and cell culture kits are available. Autoclavable vessels, 114 L, may be selected with choice of impeller, heat blanket or water-jacket heaters, and direct or magnetic-drive motors.

The basic system consists of a vessel, a Primary Control Unit for entering and displaying process parameters, and a Power Module to control temperature, agitation and pumps.

Advanced Fermentation Kits include additional modules for pH/DO and foam/level control, as well as a pump module consisting of four pumps for feeding/harvesting operations in batch, fed-batch or perfusion modes. Advanced Cell Culture Kits further add a Gas Mix controller.

All process parameters are entered and displayed on the systems Primary Control Unit (PCU). The PCU is capable of simultaneously controlling up to four individual vessels, and displaying up to 32 parameters in English, French, Spanish, or German. BioCommand Plus, a PC-compatible data acquisition and control package, is also available for comparing historical data, trend graphing, and validation support.


CELL CULTURE RUNS
Used an Advanced Cell Culture Kit with 3.0-L water-jacketed vessel, magnetic drive and a spin-filter impeller. BioCommand software was also used to control feeding and collect data. See Table 2.


FERMENTATION RUNS
Used an Advanced Fermentation Kit with direct-drive motor and 7.5L heat-blanketed vessel. BioCommand software was also used to control feeding and collect data. See Table 3.



DISCUSSION
Our results demonstrate that BioFlo 110 is a versatile tool capable of achieving high yields from a wide variety of anchorage-dependent or suspension cultures. The system allows easy substitution of control modules and interchangeable vessels and impellers. Optional data logging and control software make this a powerful research tool. The BioFlo 110 is just one in a full line of fermentors and bioreactors New Brunswick Scientific offers in 11500-L capacities for all your research through production requirements.




'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
2. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Control Your siRNA Research
5. Getting Started with MicroRNA Research
6. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
7. Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C
8. Research Organics
9. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
10. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
11. Research Tools for Analysis of ZAP-70
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... ... June 23, 2016 , ... ... its second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with experienced ... this eBook by providing practical tips, tools, and strategies for clinical researchers. , ...
(Date:6/23/2016)... Mass. , June 23, 2016   ... development of novel compounds designed to target cancer ... napabucasin, has been granted Orphan Drug Designation from ... the treatment of gastric cancer, including gastroesophageal junction ... stemness inhibitor designed to inhibit cancer stemness pathways ...
(Date:6/23/2016)... A person commits a crime, and the detective ... the criminal down. An outbreak of foodborne illness ... (FDA) uses DNA evidence to track down the bacteria that ... It,s not. The FDA has increasingly used a complex, cutting-edge ... illnesses. Put as simply as possible, whole genome sequencing is ...
(Date:6/23/2016)... Lawrence, MA (PRWEB) , ... June 23, 2016 ... ... the Peel Plate® YM (Yeast and Mold) microbial test has received AOAC Research ... test platform of microbial tests introduced last year,” stated Bob Salter, Vice President ...
Breaking Biology Technology:
(Date:6/9/2016)... leader in attendance control systems is proud to announce the introduction of fingerprint attendance ... the right employees are actually signing in, and to even control the opening of ... ... ... Photo - ...
(Date:6/2/2016)... The Department of Transport Management (DOTM) of ... project, for the , Supply and Delivery of ... Infrastructure , to Decatur , ... Management Solutions. Numerous renowned international vendors participated in the tendering ... selected for the most compliant and innovative solution. The contract ...
(Date:5/24/2016)... Ampronix facilitates superior patient care by providing unparalleled technology to leaders of ... the latest premium product recently added to the range of products distributed by Ampronix. ... ... ... Medical Display- Ampronix News ...
Breaking Biology News(10 mins):