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An Assessment of the Eppendorf twin.tec plate performance in PCR* ,,, applications

attain the set temperature (55.5 C) because of this inefficient heat transfer. This difference in temperature transfer between the plates helps to explain the results in the previous experiment about the robust product formation with the twin.tec plate.

From the foregoing discussion, we can conclude that the ideal microplates for PCR application are those that can effectively transfer heat to the reaction mixture. All of the results from the above experiments point towards this characteristic of twin.tec plates, which make them a perfect choice for all temperature-sensitive applications

Figure 2. Temperature profile of Eppendorf twin.tec plate vs a competitor PCR plate.
Green trace = twin.tec
Red trace = competitor plate

Conclusions

Eppendorf twin.tec plates offer many advantages over other PCR plates in the market. Primarily, twin.tec plates increase specific yields of some PCR products to a greater extent than the competing plates. This increase in yield is probably due to the excellent heat transfer properties of twin.tec plates, which are evidenced by the above temperature recordings. The superior performances of twin.tec PCR plates, combined with their structural rigidity, make them an excellent choice for all 96-well PCR applications.

* Practice of the patented polymerase chain reaction (PCR) process requires a license.
** The Eppendorf Thermal Cycler is an Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the l
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