( ...)An Assessment of the Eppendorf twin.tec plate performance in PCR* ,,, applications

Abstract

To evaluate the performances of Eppendorf 96-well twin.tec PCR plates, we carried out PCR reactions with twin.tec plates and PCR microplates from another commercial microplate supplier and compared the reaction products by agarose gel analysis. Additionally, simultaneous temperature profiles were recorded in both plates using dual temperature probes during the course of a PCR reaction. As a result of these experiments, we found differences in efficiencies of the amplification of a 2.0 kb target. Further, there were also differences in temperature profiles between the two plates. In this article, we present evidence to substantiate that Eppendorf twin.tec plates have excellent temperature transfer characteristics and outperform competitor plates.

Introduction

There are several suppliers of 96-well microplates for performing PCR reactions, and this format has become the most popular for high throughput research. One of the prime requirements for a good PCR microplate is the efficient transfer of block temperature to the reaction samplethis feature translates into a robust and reliable PCR reaction. Another desirable feature in a microplate is the mechanical sturdiness for unimpeded handling by robotic arm for automation. Eppendorf recently introduced the 96-well twin.tec plates to the life science research community with the goals of addressing the aforementioned requirements. Twin.tec plates represent a new generation of 96-well plates for PCR applications, which combines the advantages of two materialspolyca
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