Navigation Links
An Animal-Free DNase I

iciency of DNA degradation was monitored by comparing cycle thresholds (Ct), with and without DNase digestion. As shown in Figure 1B, both DNase I and rDNase I shifted the real-time PCR signal by an average of 13.4 Ct's, or about 11,000-fold, to less than a single copy genome equivalent (~2 pg genomic DNA).

Figure 1. Real-time PCR of Genomic DNA Samples Treated with rDNase I vs. Bovine Pancreatic DNase I. (A) Standard curve generated with 10 fold serial dilutions of human genomic DNA ranging from 100 ng to 10 pg using an ABI 7900 real-time PCR machine. The target amplified was human GAPDH. (B) GAPDH was amplified from three human genomic DNA samples (0.5 g), either treated with DNase I (Ct=36.1), treated with rDNase I (Ct =36.9), or untreated (Ct= 23.1). The two DNase I treatments gave essentially identical results and were therefore averaged and compared to the untreated sample. The Ct of the averaged DNase I treatments vs. untreated human genomic DNA was 13.4.


Lack of RNase Activity
Since rDNase I is derived from a substantially "cleaner" source than pancreatic DNase I, the enzyme can be more readily purified to a RNase-free state. Although the recommended usage conditions for DNase I rarely require more than 2U per reaction, rDNase I passes Ambion's stringent RNase contamination quality control assay using 10 times as much enzyme for a 32-fold longer incubation! This result means that rDNase I is qualified RNase-free at 320 times typical usage conditions, creating an unsurpassed margin of safety for every application.


back to top


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The Worlds Best DNase Improved TURBO DNA-free
2. TURBO DNase: Hyperactive, Salt Tolerant DNase
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Animal Free DNase

(Date:5/4/2015)... 4, 2015 /PRNewswire/ - Fluorinov Pharma (Fluorinov), a ... appointment of Dr. Roger J Garceau, to its ... companies in matters including corporate development and clinical ... adds significant regulatory expertise as Fluorinov transitions to ... 30 years of pharmaceutical development experience to Fluorinov ...
(Date:5/4/2015)... Md. , May 4, 2015  RegeneRx ... today announced that an independent team of researchers ... reported that Thymosin B4 (TB4) plays an important ... burn wounds, induced in an experimental mouse model, ... vascular remodeling of damaged dermal tissue.  The research ...
(Date:5/4/2015)... Achieved primarily through a proprietary paraffin and stain ... 100x better detail than the industry standard. But ... microscopy methods, it needed another medium to measure its ... of reference because it represents the highest standard of ... earliest discovery days of Ultralight Histology was to reverse ...
(Date:5/4/2015)... HONG KONG , May 4, 2015 ... or the "Company"), China,s leading ... cell processing and stem cell storage services, today announced ... Holdings Limited ("Golden Meditech"), a Hong Kong ... the Company, has entered into an agreement (the "Agreement") ...
Breaking Biology Technology:Roger J Garceau joins Fluorinov board, adds clinical expertise to bromodomain program and oncology pipeline 2Roger J Garceau joins Fluorinov board, adds clinical expertise to bromodomain program and oncology pipeline 3Researchers Report that Thymosin B4 has a Major Role in Dermal Burn Wound Healing 2Researchers Report that Thymosin B4 has a Major Role in Dermal Burn Wound Healing 3China Cord Blood Corporation Receives Notice of a Transaction Regarding its 7% Senior Convertible Notes Due 2017 Held by KKR 2China Cord Blood Corporation Receives Notice of a Transaction Regarding its 7% Senior Convertible Notes Due 2017 Held by KKR 3
... skills needed for the Information Age. Their administrations, as well ... way beyond the Industrial Age. , ,For the most ... not talking about reading, writing and `rithmetic; I am talking ... to regimentation, which is what was needed to assimilate people ...
... receive $1.5 million from the U.S. Department of Energy ... ethanol at paper mills by extracting the sugars from wood ... Akhtar, president of CleanTech Partners and principal investigator on the ... Other participants included the United States Department of Agriculture ...
... - The origins of Madison's technology industry can be ... isolated when he opened DNASTAR, Inc . that year. , ... - and a few more, the producer of DNA analysis ... era where it was considered bad form - even a social ...
Cached Biology Technology:Rote education fails the Information Age 2Rote education fails the Information Age 3Rote education fails the Information Age 4DNASTAR catches second wave of sequencing 2DNASTAR catches second wave of sequencing 3DNASTAR catches second wave of sequencing 4
(Date:4/2/2015)... GOTHENBURG, Sweden , April 2, 2015 ... for its touch fingerprint sensor FPC1025 from the distributor ... Deliveries will commence during Q2 2015 although the major ... 2015. The sensors will be used by smartphone manufacturers ... of 110 MSEK is included in the communicated revenue ...
(Date:3/31/2015)... NEW YORK , March 31, 2015  Elephant ... the "Company"), a global provider of Software Defined Network ... solutions, today announced total revenue of approximately $20.4 million ... to a restated $19.5 million for the year ended ... of calendar 2014, the Company had completed its multi-year ...
(Date:3/26/2015)... March 26, 2015 The Granite ... recreation and athletic club, today announced it has implemented ... freedom of movement for members and staff, while restricting ... comprehensive process, we selected FST,s IMID Access system because ... for our members and staff, in addition to unparalleled ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 110 Million 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2
... HARBOR, N.Y. For more than 25 years, ... served as an established resource for researchers, students, and ... to process normal or diseased tissues for microscopic analysis. ... ( www.scionpublishing.com ), will remain as the leading source ...
... to replace the faulty CLN2 gene, which causes a neurodegenerative ... to slow significantly the rate of neurologic decline in treated ... in the May 2008 issue (Vol. 19 No. 5) of ... Liebert, Inc. The paper is available free online at www.liebertpub.com/hum ...
... to understanding pain sensitivity - specifically why its variable ... regulates a heat-activated molecular sensor. Their description ... appears in the May 2 issue of Cell. ... want to know what molecules are involved in pain ...
Cached Biology News:Expanded histology methods book covers processing of animal, plant, and microbial tissues 2Gene therapy slows progression of fatal neurodegenerative disease in children 2Too hot to handle! Scientists identify heat sensing regulator 2
... The Dyad Disciple thermal cycler is ... It may also be operated independently, using ... variety of interchangeable Alpha units (sample-holder, heat-pump ... well as the Chromo4 real-time detector. Peltier ...
AVOID FREEZE/THAW CYCLES. Recognizes the ~32 kDa DNase IIα chain and the ~40 kDa proenzyme. DNase II causes both DNA condensation and DNA fragmentation. ...
...
BD Falcon Center-well 60 mm In Vitro Fertilization (IVF) Dish, nonembryotoxic, noncytotoxic, tissue-culture treated polystyrene. (20/sp, 500/ca) Packaging: 20 / sleeve, 500 / case ...
Biology Products: