Navigation Links
An Animal-Free DNase I


Digestion of DNA to undetectable levels is important in many molecular biology applications. For instance, accurate quantitation of RNA targets by RT-PCR requires the removal of contaminating genomic DNA targets. In vitro transcribed RNA is also frequently treated with DNase to remove template DNA in both research and pharmaceutical applications. Ambion now supplies a recombinant DNase I that is prepared in a host with little to no RNase activity. The host is non-animal derived and the enzyme is purified through a process devoid of any animal byproducts.


Isolating RNase-free DNase I is a Challenge
DNase I has historically been prepared from bovine pancreas, one of the richest sources of RNase activity (~1 mg RNase A per gram of tissue). Therefore, it is often hard to obtain DNase I sufficiently free of RNase that it will not compromise RNA analysis experiments. Providing a high quality, RNase-free DNase I is very important to Ambion. This is borne out in a recent Biotechniques article (Matthews et al, 2002) that compared several different manufacturer's DNase I products (see sidebar at right).


A Pure Source of DNase I
Ambion employs stringent purification protocols and qualifies our bovine-derived DNase I at concentrations 5X or greater than that recommended for use. Now Ambion goes a step further by providing an alternative to bovine DNase I, recombinant DNase I (rDNase I), that further eliminates residual RNase and other copurified contaminants. rDNase is a highly purified product derived from cloned bovine DNase I. The rDNase I is prepared in a host that has RNase levels that are 1 x 107 fold lower than bovine pancreas.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The Worlds Best DNase Improved TURBO DNA-free
2. TURBO DNase: Hyperactive, Salt Tolerant DNase
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Animal Free DNase

(Date:8/21/2014)... 2014 On Wednesday of last week RENU ... Redox Signaling molecules, became available for purchase in Australia and ... way that RENU 28 works is, if you think about ... the rate of cellular renewal within your body. If you ... that rate of cellular renewal. What RENU 28 does is ...
(Date:8/20/2014)... have it that American Indians largely were wiped out ... and tuberculosis brought to the New World by European ... million people lived in the Americas shortly before Europeans ... by European diseases., But new research led by anthropological ... Krause of the University of Tubingen in Germany indicates ...
(Date:8/20/2014)... High performance computing (HPC) will ... complete climate and Earth system model to address ... , Eight national laboratories, including Lawrence Livermore ... for Atmospheric Research, four academic institutions and one ... national laboratories include Argonne, Brookhaven, Lawrence Berkeley, Los ...
(Date:8/20/2014)... The 6th Annual Arrowhead Personalized ... October 29-30 in San Francisco, CA. This year’s conference ... researchers and clinicians focused on addressing and solving the ... the twelve years since the sequencing of the human ... with pharmacogenetic information in the label and molecular diagnostics ...
Breaking Biology Technology:RENU 28 Skin Care Now Available in Australia and New Zealand 2Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3New Project Is the ACME of Addressing Climate Change 2New Project Is the ACME of Addressing Climate Change 36th Annual Personalized and Precision Medicine Conference Bringing Together Thought Leaders to Discuss Clinical Implementation, Dx Reimbursement, Big Data, and Sequencing 2
... -- a North,American Advisory firm -- has been tapped ... conduct special analyses on China,s continuing,developmental efforts in specific ... on the top-tiered,areas of Beijing, Shanghai, and Tianjin, additional ... project. , (Photo: ...
... DSM,Biologics, a business unit of DSM Pharmaceutical Products, and ... Swiss Exchange:,CRX), today announced that Hungary-based Gedeon Richter Plc. signed ... biopharmaceuticals on the PER.C6(R) platform. , ... , About PER.C6(R) technology ...
... Tengion Inc., a clinical stage regenerative medicine company focused on ... M.D., President and Chief Executive Officer of Tengion, will present at ... 2008 at 9:50 a.m. EST at The Palace Hotel in New ... About Tengion , , ...
Cached Biology Technology:DSM Biologics and Crucell Announce Commercial PER.C6(R) Licensing Agreement with Gedeon Richter 2DSM Biologics and Crucell Announce Commercial PER.C6(R) Licensing Agreement with Gedeon Richter 3DSM Biologics and Crucell Announce Commercial PER.C6(R) Licensing Agreement with Gedeon Richter 4
(Date:8/21/2014)... to Wednesday, Oct. 18-22, 2014 , WHERE: ... Drive, San Diego, CA 92101 , WHAT: ... presentations of the latest research in human genetics. ... featuring high-scoring abstracts on rare genetic variants in ... and treatment for sun sensitivity (Saturday, Oct. 18, ...
(Date:8/21/2014)... University have developed a novel and versatile modeling strategy ... creating new materials as well as for studying polyelectrolytes, ... us to model much larger and more complex polyelectrolyte ... Nan Li, lead author of a paper on the ... of Materials Science and Engineering. "This is a big ...
(Date:8/20/2014)... persist in a cold, dark world. A UT microbiology ... waters and sediments from a shallow lake deep beneath ... supports microbial ecosystems. , The National Science Foundation-funded ... for life in other extreme environments, both on Earth ... in the current edition of the science journal ...
Breaking Biology News(10 mins):American Society of Human Genetics 2014 Annual Meeting 2Researchers develop models to study polyelectrolytes, including DNA and RNA 2University of Tennessee research uncovers subglacial life beneath Antarctic ice sheet 2
... As people become more globally mobile, plants can inadvertently get ... not used to hosting them. Such invasions of exotic ... expense of native plants. How weeds and invasive plants ... can manage and control them, are addressed in a newly ...
... of extended wakefulness and low-dose alcohol has significant adverse ... the risk of getting into a vehicular accident, according ... of the journal SLEEP. The study, authored by ... and Sleep in Heidelberg, Victoria, Australia, focused on 19 ...
... decades, pundits have predicted new contraceptives for men within the ... any closer? Judging from work presented today at the ... be yes. But will men actually use a new ... the nonprofit Male Contraception Information Project, says demand is the ...
Cached Biology News:Book on weeds and invasive plants discusses how to manage them using ecological approaches 2Book on weeds and invasive plants discusses how to manage them using ecological approaches 3Extended wakefulness, combined with alcohol, severely impairs driving performance 2Contraception: progress brings hope for new methods for men 2
... OEM-EPC is the smallest electronic pressure ... 60 mm. Parker configured this unit specifically ... OEM markets. Used in carrier gas flow ... liquid dispensing, and hydro-dynamic focusing, the OEM-EPC ...
... (Low Flow) offers the same benefits as the ... where control is critical or flow is required ... the flow of gas in proportion to the ... or pulse width modulation; closed loop feedback delivers ...
...
... is obtained from non-demolished blood ... fasted donors. The donors are ... and are maintained on an ... Protein Concentration : 85 mg/ml ...
Biology Products: