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Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

y for large fragment sizes, which should result in more accurate allele calling by GeneMapper software, and superior sizing precision (Figure 2) with reduced run times.

Conclusion

The Applied Biosystems capillary electrophoresis platforms, 3130/3130xl Genetic Analyzers and the 3730/3730xl DNA Analyzers, in conjunction with optimized AFLP primer kits and GeneMapper Software v3.7 or higher, provide a complete solution for performing AFLP assays. System features include high resolution, superior sizing precision, low turnaround times, and ease of use. The integration of GeneMapper software with these instruments provides one-button operation for sample loading, data collection, fragment size-calling, and accurate marker scoring in both graphical and standard binary formats. All of these features enable the generation of ample high-quality data with minimal hands-on time required and little need for manual data review.


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