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Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

s, the systems offer true onebutton operation from sample loading to data analysis. The instruments also contain several features, such as:

Enhanced sizing precision (Figure 2) from a detection cell heater that facilitates better thermal control

An automated polymer delivery system that significantly reduces set-up time and cost

Easy to use wizards for instrument operation

Automated Data Analysis with GeneMapper Software v3.7 or higher

Crucial to the successful completion of an AFLP assay is the availability of analysis software that can accurately and reproducibly score AFLP samples. GeneMapper Software v3.7 or higher offers a new AFLP analysis method and several new features for accurate and reproducible AFLP analysis. These features include the ability to use the sample files to generate the panels which are the collection of markers for that sample set. In addition, the software also contains a custom plot feature that allows multiple plots, each with a unique color, to be overlaid.

The software uses advanced analysis algorithms that can rapidly and accurately identify common and polymorphic peaks among large numbers of samples. Genotyping quality (GQ) scores flag sample data with lower quality for manual review.

When analysis is complete, the software automatically compiles the marker genotype results in a standard binary format (Table 1), which can be exported for further downstream analysis. The software also contains a convenient new Report Manager feature that generates reports containing final results that can be printed or exported for further analysis. Researchers can customize the format of these reports to meet the needs of their individual projects.

Parentage Testing with AFLP Analysis

To highlight the use of the 3130 and 3730 series platforms for AFLP anal
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