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Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

ms Plant Mapping

Kit with genomic DNA samples from maize to demonstrate that the Applied Biosystems capillary electrophoresis platforms, the Applied Biosystems 3130 series Genetic Analyzers or the Applied Biosystems 3730 series DNA analyzers in conjunction with GeneMapper v.3.7 software or higher provides a complete solution that enables researchers to perform accurate and reproducible AFLP projects in a routine fashion.

The AFLP Assay

When setting up an AFLP assay, an optimization step may be necessary to identify those primer combinations that will generate sufficient unique marker fragments for a study. The Applied Biosystems Plant Mapping Kit contains eight selective EcoRI primers for regular plant genomes, eight selective EcoRI primers for small plant genomes and eight MseI primers. The primers are labeled with either 5-FAM, NED, or JOE fluorophores (dye set F). This kit allows researchers to choose from 128 possible primer combinations that have been tested across several crop genomes, enabling them to identify the optimal pair(s) for a given organism without having to design, synthesize, or perform quality control tests of their primers.

Capillary Electrophoresis

The Applied Biosystems 3130 series Genetic Analyzers and 3730 series DNA Analyzers are fully automated, high-performance, fluorescence-based, multi-capillary systems that can run 4 (3130 Genetic Analyzer), 16 (3130xl Genetic Analyzer), 48 (3730 DNA Analyzer) or 96 (3730xl DNA Analyzer) samples. Sample analysis on these instruments is fully automated, from the moment each 96- or 384-well plate is placed on the instrument and the run is initiated. Each of the systems provide continuous, unattended operation, from automated polymer loading and sample injection to separation, detection, and data generation. When the Autoanalysis feature of GeneMapper software is used for data analysi
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