Navigation Links
Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

Introduction

Amplified fragment length polymorphism (AFLP) analysis is a genetic mapping technique that uses selective amplification of a subset of restriction enzyme-digested DNA fragments to generate a unique fingerprint for a particular genome. First developed for plant studies, AFLP analysis is used for a variety of applications, such as:

Creation of genetic maps for new species.

Determination of relatedness among cultivars

Establishment of linkage groups in crosses

Genetic diversity and molecular phylogeny studiesCreation of genetic maps for new species

Determination of relatedness among cultivars

Establishment of linkage groups in crosses

Genetic diversity and molecular phylogeny studies

The power of AFLP analysis derives from its ability to quickly generate large numbers of marker fragments for any organism, without prior knowledge of the genomic sequence. In addition, AFLP analysis requires only small amounts of starting template and can be used for a variety of genomic DNA samples.

The AFLP procedure consists of two amplification steps: a low-level or preselective amplification, followed by a more selective amplification, which generates a set of fragments that can be used as the discriminatory marker set for a particular sample (Figure 1).

Polymorphisms in band patterns map to specific loci, allowing individuals to be genotyped or differentiated based on the alleles they carry.

Performing Successful AFLP Analysis

The success of an AFLP assay depends on three factors:

1. Optimized reagents

2. A robust and reliable electrophoresis platform

3. Effective analysis software In this Application Note, we will use the Applied Biosyste
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fractionating DNA Fragments Generated by Differential Display PCR
2. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
3. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
4. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
5. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
6. Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping
7. Production of Full-Length, Biologically Active MEK1WT and MEK1CA
8. Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
9. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
10. Epitope-Tagging Vectors for Functional Analysis in Yeast
11. Map and Link Human Genetic Disorders with SSLP Analysis
Post Your Comments:
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... 22, 2014 Fertility Associates of Memphis ... Bank USA , a network of more than 20 ... partnership, patients have fast access to a national pool ... IVF . Fertility Associates of Memphis is the only ... treatment option. , Dr. William Kutteh , director ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
(Date:12/19/2014)... Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... that they have been acquired by Roche (SIX: RO, ... privately held company that provides a big data platform ... (NGS) data for the academic and translational research markets. ... and will continue to focus on development of their ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... D. Michael Olive, Ph.D. , ... , Lincoln, NE 68504 , ... , ... of cellular,processes in the context of a living animal. While several optical imaging modalities ...
... , INTRODUCTION ... of purity of DNA samples is spectrophotometric measurement at 260 and 280nm (1) ... g/mL will have an absorbance at 260nm of 1.0 and that the ratio of ... (2) . If a sample has an A 260 / A 280 ...
... , , ... Many laboratory protocols require the serial,dilution of reagents ... and,assay development procedures as well as standard,curve generation involve the serial dilution,of ... handling equipment with serial,dilution capabilities., ...
Cached Biology Technology:Near Infrared Technology and Optical Agents For Molecular Imaging 2Near Infrared Technology and Optical Agents For Molecular Imaging 3Near Infrared Technology and Optical Agents For Molecular Imaging 4Near Infrared Technology and Optical Agents For Molecular Imaging 5Near Infrared Technology and Optical Agents For Molecular Imaging 6Near Infrared Technology and Optical Agents For Molecular Imaging 7EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY 2EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY 3EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY 4Serial Dilution with the Bravo Liquid Handling Platform 2Serial Dilution with the Bravo Liquid Handling Platform 3Serial Dilution with the Bravo Liquid Handling Platform 4Serial Dilution with the Bravo Liquid Handling Platform 5Serial Dilution with the Bravo Liquid Handling Platform 6Serial Dilution with the Bravo Liquid Handling Platform 7Serial Dilution with the Bravo Liquid Handling Platform 8
(Date:12/19/2014)... , Dec. 18, 2014 Research and ... the addition of the "iPhone 5S Fingerprint ... Report" report to their offering. ... the acquisition of AuthenTec in July 2012, Apple ... 5S. It is currently the only device of ...
(Date:12/17/2014)... Dec. 15, 2014 Research and Markets ... of the "Samsung Galaxy S5 - Home ... to their offering. ... different sensing technology than the iPhone 5S, Samsung ... in its product. The Galaxy S5 ...
(Date:12/17/2014)... , Dec. 16, 2014 Research and Markets ... addition of the "Global Chemical Sensor Market ... http://photos.prnewswire.com/prnh/20130307/600769 One ... increasing demand for medical sensors in biomedical applications. ... for quick and correct diagnosis during surgical procedures. ...
Breaking Biology News(10 mins):iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 2Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2
... FLResearchers at the University of Florida have introduced ,Delicious, ... high yields, and is disease-resistant. The black fruit features ... it well-suited for fresh fruit consumption and the potential ... on the comments of vineyard visitors who sampled the ...
... population growth in southern Nevada has placed increasing demands ... state,s residents lived in southern Nevada, where the population ... population boom and a climate that features frequent severe ... to investigate alternative water resources to meet resident and ...
... component of good health. Like plants that thrive in the ... for the production of vitamin D. In the past few ... exposure. From fear of the potential dangers of UV radiation ... and recreational environments centered on virtual reality, we are all ...
Cached Biology News:Keeping golf courses green when fresh water is limited 2Keeping golf courses green when fresh water is limited 3The sunlight solution for better health 2The sunlight solution for better health 3
Mouse/Rat WNK1 Affinity Purified Polyclonal Ab...
Recalibration of HL-2000-CAL products...
The Zymo-Spin PI Fast-Spin column features durable polypropylene construction and is the same column featured in the His-Spin Protein Miniprep. Capacity is 800 l....
LAL Chromogenic Endpoint assay...
Biology Products: