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Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using ,,, Eppendorf MasterTaq

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Figure 1: The use of MasterTaq for amplification of the amoA gene in two ammonia-oxidizing bacterial cultures
Lane 1 - Molecular weight marker VIII
Lane 2 - Negative control (no DNA)
Lane 3 - Nitrosococcus oceani with no Ta q M a s t e r
Lane 4 - Nitrosococcus oceani with 2.5 l Ta q M a s t e r
Lane 5 - Nitrosococcus oceani with 5 l Ta q M a s t e r



References

Alzerecca, J.J., J.M. Norton, and M.G. Klotz. 1999. The amo operon in marine, ammonia-oxidizing gamma-proteobacteria. FEMS Microbiology Letters 180:21-29.

Holmes, A. J., A. Costello, M. E. Lidstrom, and J. C. Murrell. 1995. Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related. FEMS Microbiology Letters. 132:203-208.

Rotthauwe, J. H., K. P. Witzel, and W. Liesack. 1997. The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Applied and Environmental Microbiology. 63:4704-4712.

Sayavedra-Soto, L.A., N.G. Hommes, J.J. Alzerreca, D.J. Arp, J.M. Norton and M.G. Klotz. 1998. Transcription of the amoC, amoA and amoB genes in Nitrosomonas europaea and Nitrosospira sp. NpAV. FEMS Microbiology Letters 167:81-88.




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