Navigation Links
Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using ,,, Eppendorf MasterTaq

.

Figure 1: The use of MasterTaq for amplification of the amoA gene in two ammonia-oxidizing bacterial cultures
Lane 1 - Molecular weight marker VIII
Lane 2 - Negative control (no DNA)
Lane 3 - Nitrosococcus oceani with no Ta q M a s t e r
Lane 4 - Nitrosococcus oceani with 2.5 l Ta q M a s t e r
Lane 5 - Nitrosococcus oceani with 5 l Ta q M a s t e r



References

Alzerecca, J.J., J.M. Norton, and M.G. Klotz. 1999. The amo operon in marine, ammonia-oxidizing gamma-proteobacteria. FEMS Microbiology Letters 180:21-29.

Holmes, A. J., A. Costello, M. E. Lidstrom, and J. C. Murrell. 1995. Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related. FEMS Microbiology Letters. 132:203-208.

Rotthauwe, J. H., K. P. Witzel, and W. Liesack. 1997. The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Applied and Environmental Microbiology. 63:4704-4712.

Sayavedra-Soto, L.A., N.G. Hommes, J.J. Alzerreca, D.J. Arp, J.M. Norton and M.G. Klotz. 1998. Transcription of the amoC, amoA and amoB genes in Nitrosomonas europaea and Nitrosospira sp. NpAV. FEMS Microbiology Letters 167:81-88.




'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
8. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
9. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
10. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
11. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
Post Your Comments:
(Date:1/15/2014)... January 15, 2014 This webinar ... on nonclinical and clinical safety assessment in biosimilars. ... guide a path for biosimilar drug development, however ... of biosimilarity with regards to quality, safety and ... of biosimilar drug development and registration, development strategies ...
(Date:1/15/2014)... NC (PRWEB) January 15, 2014 ... an enhancement to its Online Web Portal for Life ... now have the ability to specify the subject matter ... nearly 50 life science sub-domains. This will help reduce ... and scientific fields, and decrease the likelihood of error. ...
(Date:1/14/2014)... Doylestown, PA (PRWEB) January 14, 2014 ... – 11 p.m. , Location: Warrington Country Club, 1360 ... Hepatitis B Foundation, the only national nonprofit organization solely ... improving the quality of life for those affected worldwide, ... 11 at Warrington Country Club in Warrington, Pa. ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... initiated coverage of Alliqua, Inc. (OTCQB: ALQA). Alliqua ... and marketing proprietary products to serve the wound ... , In late 2012, Alliqua was restructured ... launched the company’s new strategy to become a ...
Breaking Biology Technology:Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... DOR BioPharma, Inc. (DOR or the ... announced today that investigators at the Fred Hutchinson Cancer ... in Bone Marrow Transplantation in which they ... or BDP) decreases non-infectious inflammation of the lung in ...
... 29 /PRNewswire-Asia/ -- RainEarth Inc.,(OTC Bulletin Board: RNER; "the ... and Earth Technology Development Corp. Ltd.,("China RainEarth") attended the ... , A good marketing strategy ... products. In the forum, participants explored various marketing,strategies and ...
... Associates | Architects ( www.kornberg.com ) has been ... Cognitive & Neurobiological Imaging Center. Ken Kornberg, president and ... Stanford University since 1980 and has completed 20 ... the two-story, 32,000 square-foot office building for the Stanford Linear ...
Cached Biology Technology:DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 2DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 3DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 4DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 5DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 6RainEarth (RNER) Continues to Expand its Marketing Force in China 2RainEarth (RNER) Continues to Expand its Marketing Force in China 3Kornberg Associates Architects Selected to Develop Design for New Stanford University Imaging Center 2
(Date:4/14/2014)... scientists are continuing to unravel the molecular changes that ... respiratory infections. , When the bacterium Yersinia pestis enters ... is 100 percent fatal if untreated. The way in ... and kills people in a matter of days has ... study demonstrating that the presence of a protein called ...
(Date:4/14/2014)... families face the decision of whether to get ... the decision can be even more challenging. Now, ... ownership decisions in families of children with autism ... the parents reported the benefits of dog ownership ... children to learn responsibility. , "Children with autism ...
(Date:4/14/2014)... feel when an elevator zips you up multiple floors in ... carried you all the way up to the top of ... That,s similar to what many fish experience when they travel ... change in pressure is simply too big, too fast, and ... in the March issue of the journal Fisheries , ...
Breaking Biology News(10 mins):Plague alters cell death to kill host 2Dog ownership benefits families of children with autism, MU researcher finds 2Making dams safer for fish around the world 2Making dams safer for fish around the world 3Making dams safer for fish around the world 4Making dams safer for fish around the world 5
... trial called the "Evaluate the Clinical use of vitamin ... Angela Cheung and colleagues at the University of Toronto ... did not protect against age-related bone mineral density (BMD) ... Medicine , the findings also suggest that vitamin K1 ...
... University Medical Center has suggested an entirely new way to explain ... have debated for the past 100 years about why -- when ... object -- the flash is perceived to lag behind the position ... to present a completely different way of thinking about how this ...
... their chimpanzee relatives, bonobo societyin which females enjoy a ... image. While chimpanzee males frequently band together to hunt ... to restrict what meat they do eat to forest ... to a study, reported in the October 14th issue ...
Cached Biology News:Duke team explains a longtime visual puzzler in new way 2Despite 'peacenik' reputation, bonobos hunt and eat other primates too 2
... (eIF4E-binding protein II) is involved in translation ... kidney. PHAS proteins bind to eIF-4E, the ... capped mRNA in vitro and in cells. ... of PHAS/eIF-4E complexes. This allows eIF-4E to ...
... 2 (VMAT2). An antibody made to the ... at ~55 kDa in postnuclear supernatants of ... wild type cells (2). Some additional bands, ... also detected and remain unaffected by the ...
... Anti-Matrix Metalloproteinase-2, Hinge Region By immunoblotting ... native protein. It reacts with free MMP-2 ... antibody buffered aqueous glycerol solution ... pH 7.4, containing 50% glycerol and 15 ...
Acetylcholinesterase from human and monkey....
Biology Products: