The enlarged area shows signal color range when using cyanine probes
Cye3 spleen and Cye5 liver. Green, red, and yellow spots indicate positive
hybridization from the labeled probes. Green (Cye3) and red (Cye5) spots
indicate that the targets are expressed differentially.
For generation of PCR amplicons for microarray analyses, the Taq from the Eppendorf MasterTaq kit performs exceptionally well, even when used at one-half of the recommended concentration. High yields of specific amplicons are obtained which can be used to generate high quality microarrays.
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