Navigation Links
Amplification of Mouse cDNAs for Microarrays Using the Eppendorf ,,, MasterTaq Kit

liver and spleen polyA+ RNAs

The final experiment performed was to verify that the PCR products amplified using the Eppendorf MasterTaq Kit generated strong specific signals in a microarray experiment. We printed 1,152 PCR products representing mouse cDNA clones and appropriate controls on a reflective slide (APBiotech, UK). Fluorescent probes were synthesized from mouse spleen and polyA+ RNAs and were used to screen the microarray (Fig. 3). Green, red, and yellow spots were observed, indicating positive hybridization of the labeled probes to the printed PCR products. The enlarged area shows the signal color range observed when using cyanine probes, Cye3 spleen, and Cye5 liver. Green (Cye3) and red (Cye5) spots indicate differential expression of these particular mRNAs sequences in spleen and liver respectively, whereas yellow spots indicate similar levels of expression in both tissues.

1152 cDNA clones derived from the mouse uniqene set and controls were amplified with the Eppendorf MasterTaq Kit and printed on derivatized glass slides. Fluorescent probes were synthesized from spleen and polyA+ RNAs and used to screen the microarray. The enlarged areas shows signal color range when using cyanine probes, Cye3 spleen and Cye5 liver. Green, red, and yellow spots indicate positive hybridization from the labeled probes. Green (Cye3) and red (Cye5) spots indicate that the targets are expressed differentially. Figure 3
The 1,152 cDNA clones derived from the mouse Unigene set and controls were amplified with the Eppendorf MasterTaq Kit and printed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
9. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
10. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
11. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
Post Your Comments:
(Date:10/20/2014)... , October 20, 2014 , ... (GI) disorders and the delivery of care has revealed ... and worrying inequalities in the provision of healthcare services ... was commissioned by United European Gastroenterology (UEG), have been ... and public awareness of the burden of GI disorders ...
(Date:10/20/2014)... 20, 2014 PureTech , a science ... healthcare problems, announced today the closing of a ... from Invesco Perpetual, a $120 Billion group of ... PureTech,s existing pipeline forward and to advance new ... scientific creativity to really go for the big ...
(Date:10/20/2014)... Virginia (PRWEB) October 20, 2014 ... NDA Partners LLC, announced today that Ellen Teplitzky, ... legal support for the pharmaceutical industry, has joined ... manager of its legal services practice. NDA ... and expert witness and testimony, to top law ...
(Date:10/19/2014)... 19, 2014 The Latin American hardware ... in Latin America with analysis and forecast of revenue. ... and is expected to reach $2,366.8 million by 2018, ... , Browse through the TOC of the Latin American ... the in-depth analysis provided. It also provides a glimpse ...
Breaking Biology Technology:Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 2Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 3Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 4Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 3
... chooses AMICAS to drive improvements in customer ... service and operational efficiency, ... leader in radiology and medical image and information management,solutions, today ... California,s most influential radiology groups., (Logo: http://www.newscom.com/cgi-bin/prnh/20060202/AMICASLOGO ...
... July 28 Ranbaxy,Pharmaceuticals Inc. (RPI), a ... Gurgaon, India announced today that under an,agreement ... authorized,generic of Omeprazole 40 mg Capsules in ... indicated for the treatment of acid-related disease,including ...
... Pa., July 25 BioTrends Research Group, Inc.,released ... offering comprehensive insight into the management of,renal anemia ... Disease,(CKD) and Dialysis. The report provides information from ... online survey fielded in,June 2008., In the ...
Cached Biology Technology:Arcadia Radiology Medical Group Chooses AMICAS 2Arcadia Radiology Medical Group Chooses AMICAS 3Ranbaxy Launches Omeprazole 40 mg Capsules 2BioTrends Releases Quarterly TreatmentTrends(TM): Nephrology Report Highlighting Market Trends for Renal Anemia, Hyperphosphatemia, and Secondary Hyperparathyroidism 2BioTrends Releases Quarterly TreatmentTrends(TM): Nephrology Report Highlighting Market Trends for Renal Anemia, Hyperphosphatemia, and Secondary Hyperparathyroidism 3
(Date:10/14/2014)... LA JOLLA, CA – October 14, 2014 – Scientists ... $6.6 million from the National Institutes of Health (NIH) ... most prevalent virus-induced hemorrhagic fever disease in Africa. The ... disease and why some patients die, while others survive ... researchers to understand the basic mechanism of how Lassa ...
(Date:10/14/2014)... team of researchers, led by the Chinese Academy of ... Nature Genetics a brief genomic history of tomato ... tomato plant. , The C.M. Rick Tomato Genetics Resource ... this study by providing seed of both cultivated tomato ... builds on the first tomato genome sequence completed just ...
(Date:10/14/2014)... October 14, 2014 – Over the past decades, we ... institutions to meet the needs of infants for social ... these infants to thrive. , Infancy and ... of the cortex. A generation of research suggests that ... cortical development and cognitive function. In contrast, deprivation and ...
Breaking Biology News(10 mins):Scripps Research Institute team receives $6.6 million to investigate deadly Lassa virus 2Institutional rearing may increase risk attention-deficit disorder 2
... less be as simple as leaving serving dishes on the stove ... from Cornell University, it can. At this week,s ... director of the Cornell Food and Brand Lab, shared findings of ... "We looked at whether serving foods from the kitchen counter, instead ...
... and CAMBRIDGE (April 26, 2010) Pericytes, the contractile ... angiogenesis, the "sprouting" of new blood vessels, according to ... the Massachusetts Institute of Technology (MIT). The study, published ... among the first to examine mechanical signaling by pericytes ...
... A new study links progression of a lethal type ... immune system genes, suggesting how complex the immune response is ... immune system proteins for treatment. Previous research found ... with this type of brain cancer, called glioblastoma multiforme. However, ...
Cached Biology News:Cellular mechanical forces may initiate angiogenesis 2Brain tumor growth linked to lowered expression of hundreds of immune function genes 2Brain tumor growth linked to lowered expression of hundreds of immune function genes 3
... The Model 1575 immunowash microplate washer 100-240 ... that have flat-, U-, or V-bottom wells. ... horizontal and vertical needle positions to an ... crosswise aspiration, and overflow washing. It can ...
... Incubator is a compact benchtop and ... microprocessor controller with a large display ... agitation speed. Air circulation inside to ... Temperature can be easily calibrated from ...
... RM is specifically designed as a high ... new variable mass compensation allows high shaking ... better aeration and, therefore, faster growth and ... is ideal when high reproducibility is critical ...
...
Biology Products: