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Amplification of Mouse cDNAs for Microarrays Using the Eppendorf ,,, MasterTaq Kit

f PCR product includes plentiful amounts of DNA available for spotting, a reduction in cost and labor, and ultimately the production of greater numbers of arrays per PCR reaction.

Comparison of the efficiency of amplification of thermostable polymerases from bacterial cultures
Eppendorf MasterTaq and ten other polymerases were evaluated for the ability to amplify directly from overnight bacterial cultures. Approximately 1 l of the overnight culture was used to evaluate each enzyme in a reaction volume of 50 l. The main criteria for evaluation of the polymerases were specific amplification and maximum yield of the desired PCR product (Fig. 1).

Figure 1
The Eppendorf MasterTaq (#2) and ten other polymerases were evaluated for the ability to amplify directly from overnight bacterial cultures.

Polymerases:

1. Eppendorf Taq
2. Eppendorf MasterTaq
3. Competitor A
4. Competitor B
5. Home Brew
6. Competitor C
7. Competitor D
8. Competitor E
9. Competitor F
10. Competitor G
11. Competitor H


Upon comparison of ten enzymes, it was observed that enzymes 5 and 6 failed to generate specific products. Enzymes 1, 2, 3, 4, and 7 all performed well in terms of yield of PCR product and absence of non-specific amplicons, but enzyme 2 had no failures. The use of enzymes 8, 9, 10, and 11 all resulted in the amplification of extraneous bands. Given that enzyme 2 (Eppendorf
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