Navigation Links
Amplification of Mouse cDNAs for Microarrays Using the Eppendorf ,,, MasterTaq Kit

After printing, the microarrays were allowed to dry completely at ambient conditions. The slides were pretreated with 2x SSPE, 0.2% SDS at 55C. The cDNA probe was lyophilized and redissolved in ~32 l Microarray Hybridization Buffer Version 2 (APBiotech, UK). The solution was added to the microarray and a coverslip was applied. Hybridization was allowed to proceed for 1418 hours at 42C. The microarray was washed with 1X SSC/0.2% SDS for 5 minutes at 45C followed by two 5-minute washes with 0.1X SSC/0.2% SDS at room temperature. The microarray was rinsed briefly with water, dried with nitrogen, and scanned using a Molecular Dynamics Generation III scanner.

Results and Discussions

For the analysis of gene expression in eukaryotes, expressed sequence tag (EST) data represent an invaluable resource for gene identification. EST's are single pass partial cDNA sequences. In general, cDNA clones are selected to represent as many unique transcripts as possible. One such method to group transcripts is the Unigene clustering of data sets which attempts to identify unique human transcripts within EST data (http://www.ncbi.nlm.nih.gov/UniGene). In amplifying EST collections for the production of microarrays, the yield of each PCR amplicon is a very important consideration. The optimal concentration of DNA for successful microarray experimentation is typically 200 g/ml. Hybridizations using non-covalent attachment chemistries (e.g. polylysine, aminosilane) generally work poorly with DNA spotted at concentrations lower than this. When amplifying thousands of clones, the main objective is to minimize the total number of reactions needed to generate sufficient material for printing. A high yield o
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
9. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
10. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
11. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
Post Your Comments:
(Date:8/20/2014)... , Aug. 20, 2014 /PRNewswire-iReach/ -- A ... transformative collaboration between the school,s bioengineering department and ... of the Intel® Software Academic Program, UCSD,s research ... and the human body.  Photo ... study exhibits the work of Dr. Todd ...
(Date:8/20/2014)... August 20, 2014 GraphDB™ ... today including improvements to the enterprise replication cluster, ... for Lucene, SOLR and Elasticsearch. This release happens ... high performance triplestore – GraphDB™ was formerly known ... deploying the only mature enterprise resilient RDF triplestore ...
(Date:8/19/2014)... -- Research and Markets  has announced the addition of the ... report to their offering. This report analyzes ... Thousands by the following Product Segments: Media, Sera, and Reagents. ... Canada , Japan , ... Latin America . Annual estimates and forecasts are provided for ...
(Date:8/19/2014)... , Aug. 19, 2014 Research and ... Fuel Cells (SOFCs) - Global Strategic Business Report" report ... worldwide markets for Solid Oxide Fuel Cells (SOFCs) in US$ ... Canada , Europe , ... estimates and forecasts are provided for the period 2012 through ...
Breaking Biology Technology:Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Global Cell and Tissue Culture Supplies - Strategic Business Report 2014 2Global Solid Oxide Fuel Cells (SOFCs) - Strategic Business Report 2014 2
... SAN FRANCISCO, Calif., Nov. 1, 2010 diaDexus, Inc. ... company focused on the development and commercialization of patent-protected ... in cardiovascular disease, today announced that the company, formerly ... Bulletin Board under the ticker symbol (VXGN), has been ...
... With... -- BOZEN, Sud-Tirol, Italy, November 1, 2010 ... ... if (typeof(lpUnit)==,undefined,) var lpUnit=,PRN,; --> ... var s = ,; var first_result; // Results are keyed by longUrl, so we need to grab the first ...
... 30 Tibotec Pharmaceuticals (Tibotec) announced today the company will ... the phase 2 response-guided PILLAR study in treatment-naive patients with ... Annual Meeting of the American Association for the Study of ... be presented as part of a late-breaker oral presentation on ...
Cached Biology Technology:diaDexus Announces Name Change From VaxGen 2diaDexus Announces Name Change From VaxGen 3Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 2Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 3Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 4Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 5Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 6Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 7Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 8Health Robotics Continues Worldwide Consolidation by Entering the Norwegian Market With Beckman Automation 9Week-24 Interim Results From Phase 2b PILLAR Study to be Presented as Late-Breaker at AASLD 2Week-24 Interim Results From Phase 2b PILLAR Study to be Presented as Late-Breaker at AASLD 3Week-24 Interim Results From Phase 2b PILLAR Study to be Presented as Late-Breaker at AASLD 4
(Date:8/20/2014)... are visible in in this image of the Para ... these were most likely intentionally set in order to ... forest or stand of trees where the land is ... include conversion of forestland to farms, ranches, or urban ... green of the Amazon Rainforest in the middle of ...
(Date:8/20/2014)... Area Lyme Foundation, which aims to make Lyme disease ... research published in an upcoming issue of the Elsevier ... The findings show that ticks that carry Lyme ... making the threat of Lyme disease year-round. The research ... Health (CDPH) Vector-borne Disease Section and University of California, ...
(Date:8/20/2014)... Research UK scientists have discovered that acral melanomas the ... Marley,s death are genetically distinct from other more common ... external) published in the journal Pigment Cell & Melanoma ... of the hands, soles of the feet, nail-beds and other ... of melanoma, it,s not caused by UV damage from the ...
Breaking Biology News(10 mins):Lyme disease risk is year-round in Northwest California, according to new study 2Lyme disease risk is year-round in Northwest California, according to new study 3Scientists learn more about rare skin cancer that killed Bob Marley 2
... Pharmaceuticals report this week that they have successfully used RNA ... mice, an advance that could lead to new treatments for ... 1998 discovery of RNA interference the naturally occurring phenomenon ... nucleus to the protein-building machinery of the cell is disrupted ...
... New Delhi, Singapore and Washington DC (Dec 28, 2010) ... identify with political parties , according to an article in ... official journal of the Western Political Science Association (published by ... studied concepts in modern political science. Scholars have long held ...
... spotlight that illuminates an otherwise dark scene, attention brings to ... A new study by researchers at the Salk Institute for ... that primarily had been known for its role in the ... the mind,s spotlight. Their findings, published in the Dec. ...
Cached Biology News:New RNA interference technique can silence up to 5 genes 2Seeing without looking 2
Black Polypropylene 384 Round Well Plate 70/Box...
... Rabbit polyclonal to Peroxiredoxin 1 ... peroxidase family, whose mammalian members have ... proliferation, differentiation, and apoptosis. Many isoforms ... to the amino acid sequence homology, ...
... Eliminates nonspecific products due to inactivity ... allows extension through DNA regions which contain ... an 'A' overhang ,TEMPase II is ... Polymerase that remains inactive at room ...
... Synuclein Synucleins are small, soluble proteins ... certain tumors. The family includes three known ... synuclein. The alpha and beta synuclein proteins ... they are seen mainly in presynaptic terminals. ...
Biology Products: