Navigation Links
Amplification of Mouse cDNAs for Microarrays Using the Eppendorf ,,, MasterTaq Kit

Katrine Verdun, Richard Rouse, and Gary Hardiman
BIOGEM, Division of Biology, University of California San Diego, La Jolla, CA 92093-0349
e-mail: ghardiman@ucsd.edu
www.biogem.ucsd.edu

Introduction

Microarray technology, primarily advanced by Pat Brown and his colleagues at Stanford University, makes use of a robotic spotting device or "microarrayer" to spot DNA sequences onto "derivatized" glass slides (1). These arrays are then hybridized with cDNA probes and analyzed using methods conceptually similar to those described for Affymetrix gene chips (2). Since the cost of producing an individual array is relatively small, spotted arrays are highly versatile and can make use of a wide variety of clone sets. Microarrays thus enable individual investigators to perform large-scale analysis of model organisms or to customize arrays for special genome applications.

The cDNAs spotted on microarrays are typically PCR products, amplified by PCR from plasmid miniprep DNA of cDNA clones. Amplification directly from clones in culture has been recently reported (3). This is more advantageous in high-throughput applications in that it is more cost efficient, considerably less labor intensive, and minimizes sample cross-contamination. The recommended concentration for spotting on aminosilane and polylysine slides is 200400 fmol/l. Lower concentrations than this will result in poor signals, and higher concentrations are not recommended as target spots are seen to smear. Microarray hybridizations work poorly with weak PCR products. Consequently, PCR amplification is optimized to maximize DNA yield. In this report w
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
9. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
10. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
11. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
Post Your Comments:
(Date:10/22/2014)... According to new market research report ... Integration, Deployment & Support), by Service Model (SaaS, ... Others (Hybrid & Community)) - Global Forecast to ... Cloud Professional Services Market into various sub-segments with ... also identifies the drivers and restraints for this ...
(Date:10/22/2014)... HONG KONG , Oct. 22, 2014 ... enterprise, announced today that rare disease expert John ... president, research. Dr. McKew brings more than two decades ... at the National Institutes of Health, Wyeth Research and ... McKew will lead aTyr,s efforts to expand and translate ...
(Date:10/20/2014)... GUELPH, ON , Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. ... totalling $1.7 million which brings Q3 orders to $5.8 million and ... odour control projects in North America and ... "We continue to bid new projects at record levels," said ... values were higher at the end of Q3 than they have ...
(Date:10/20/2014)... Earle Martin , Chief ... that Ellen Teplitzky, an experienced attorney specializing in ... industry, has joined the firm as Director of ... practice. NDA Partners provides legal services, including ... to top law firms and their clients in ...
Breaking Biology Technology:Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2
... 14 /PRNewswire-FirstCall/ - BioSyntech, Inc. (TSX: BSY), a ... today announced that it will conclude enrolment next ... cartilage repair device, BST-CarGel(R). To date, the Company ... clinical sites will be permitted to enrol additional ...
... Va., Jan. 14 CEL-SCI CORPORATION , ... reports financial results for the fiscal year ended September 30, 2008.CEL-SCI ... a net loss of $(9,629,657) in fiscal year 2007. The ... $(0.07) compared to a net loss per common share in fiscal ...
... Comprehensive tutorials on the publicly available PROSITE, InterPro, IntAct and UniProt ... , ... Seattle (PRWEB) January 15, 2009 -- ... PROSITE, InterPro and IntAct, in addition to a newly updated tutorial ...
Cached Biology Technology:BioSyntech to conclude enrolment for pivotal BST-CarGel(R) trial in February 2009 2BioSyntech to conclude enrolment for pivotal BST-CarGel(R) trial in February 2009 3BioSyntech to conclude enrolment for pivotal BST-CarGel(R) trial in February 2009 4CEL-SCI Corporation Announces 2008 Financial Results 2CEL-SCI Corporation Announces 2008 Financial Results 3New and Updated Online Tutorials for PROSITE, InterPro, IntAct and UniProt 2New and Updated Online Tutorials for PROSITE, InterPro, IntAct and UniProt 3
(Date:10/14/2014)... N.Y. – Scientists have sequenced the house fly genome ... one might expect from an insect that thrives in ... published Oct. 14 in the journal Genome Biology ... biology and of how flies quickly adapt to resist ... Adult house flies (Musca domestica) carry and transmit more ...
(Date:10/14/2014)... fish oil supplements, rich in omega-3 fatty acids, do not ... which the heart can beat as fast as 150 beats ... the Montreal Heart Institute were published in the Journal ... , For the trial, 337 patients with atrial fibrillation not ... of fish oil a day or to placebo for up ...
(Date:10/14/2014)... by the Chinese Academy of Agricultural Sciences in Beijing, ... brief genomic history of tomato breeding, based on sequencing ... C.M. Rick Tomato Genetics Resource Center at UC Davis ... seed of both cultivated tomato varieties and related wild ... tomato genome sequence completed just two years ago, shows ...
Breaking Biology News(10 mins):House fly genome reveals expanded immune system 2
... MA (July 27, 2011) SomaLogic, Inc. and New ... entered into an exclusive licensing agreement whereby SomaLogic will ... several current and future NEB nucleic acid amplification and ... specific protein binding reagents based on chemically modified "aptamers," ...
... each other plant and harvest, rural Chinese neighbors also ... likely to reenroll their land in a conservation program ... Scientists from the Center for Systems Integration and Sustainability ... study the amount of land farmers in the Wolong ...
... NEW YORK (July 27, 2011) ... increasingly sophisticated illegal trade in wildlife parts conducted by organized crime, coupled ... rhinos, tigers, and elephants on a scale never before seen. , ... paper, published June 7 on the online issue of the journal ...
Cached Biology News:Farmers more likely to be green if they talk to their neighbors, according to MSU research 2Organized crime is wiping out wildlife 2
Whole rabbit blood (any breed, either sex) is collected at the time of slaughter and processed immediately at 4C, bottled, frozen and stored at -70C....
GlutaMAX-I Supplement...
Chemically Defined Lipid Concentrate...
... are membrane-filtered isotonic and enzyme-free aqueous ... cell-conditioning agents in either Ca2+- and ... Ca2+- and Mg2+-free phosphate-buffered saline ... from support substrates and each other ...
Biology Products: