Navigation Links
Amplification Efficiency of TaqMan Gene Expression Assays

TaqMan Gene Expression Assays average 100% efficiency (+/- 10%).

Abstract

Applied Biosystems has designed and manufactured over 40,000 real-time PCR assays for measuring the expression of genes in humans, mice, and rats. TaqMan Gene Expression Assays each consist of amplification primers (forward and reverse) and a fluorescent labeled TaqMan probe, formulated into a single tube.

One of the major user concerns regarding any real-time PCR-based assay product (primers and TaqMan probe) is the amplification efficiency of the PCR reaction. Applied Biosystems has done extensive R&D validation of our TaqMan Gene Expression Assays to address this concern.

Two types of experiments have been performed to evaluate the amplification efficiency of TaqMan Gene Expression Assays. First, we determined the amplification efficiency of a large sampling of assays using statistically relevant methods. Second, we evaluated the effect of amplification efficiency on the ability to determine a difference in the relative quantity of a transcript expressed in two different samples. Our results indicate that the amplification efficiency associated with assays designed using our informatics pipeline is 100%. Additionally, our results show that the ΔΔCT method for relative quantitation is better correlated with expected fold change in gene expression than methods using predetermined amplification efficiency values for individual assays.

Introduction

A key attribute of any real-time PCR reaction is the amplification efficiency of the PCR. Specifically, is there a doubling of PCR product at every PCR cycle1,2? The equation that describes the exponential amplification of PCR is:

For real-time amplification using TaqMan probes, Xn is proportional to the repor
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Amplification Efficiency TaqMan Gene Expression Assays

(Date:1/14/2014)... In recent years, growing suspicion about the ... development and promotion has led to unprecedented levels of ... concerns about the insidious impact of commercialization of research, ... to the world’s biggest pharmas for illegal marketing activities, ...
(Date:1/14/2014)... OTTAWA, Ontario , Jan. 14, 2014   Kinaxis ®, ... ( SCM ) and sales and operations planning ( S&OP ... the Biomanufacturing Summit , which will be held at ...  At the conference, join Kinaxis customer Elisabeth Kaszas , ...
(Date:1/14/2014)... 2014 During the 1600’s through the 1800’s ... Doctor’s Plague.” In this time period, doctors did not know ... times, to the death of vulnerable patients. In the same ... they may be unwittingly transmitting herpes viruses to their patients. ...
(Date:1/14/2014)... Holloway America today announced an addition to ... (MTRs) for all pressure vessels and tank parts the ... important for equipment that must meet ASME, CE, PED, ... functionality that allows authorized users to quickly locate and ...
Breaking Biology Technology:The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 3Pressure Vessel Design and Fabrication Company Builds Database for Material Test Reports 2Pressure Vessel Design and Fabrication Company Builds Database for Material Test Reports 3
... Symbol: MS , , , ... Medical Corp. (TSX: MS), today announced that the University of Alberta , a major shareholder ... , , Certain employees, officers ... Alberta . , , , ...
... Smartfield, ... was approved for $250,000 for completion and commercialization of their SmartCrop® technology. SmartCrop is ... , ... 30, 2010 -- Smartfield, Inc. is an information company that develops and ...
... ATLANTA , April 29 Xoft, Inc., developer of ... therapy directly to cancer sites with minimal radiation exposure to surrounding ... for the treatment of skin cancer. The announcement was made here ... April 29-May 1 . , ...
Cached Biology Technology:BioMS Medical updates University of Alberta equity position 2$250,000 Awarded to Smartfield, Inc. of Lubbock, TX by the Texas Emerging Technology Fund 2Xoft's Electronic Brachytherapy Hits Milestone With 10 New Skin Cancer Installations 2Xoft's Electronic Brachytherapy Hits Milestone With 10 New Skin Cancer Installations 3Xoft's Electronic Brachytherapy Hits Milestone With 10 New Skin Cancer Installations 4
(Date:4/22/2014)... 2014 Scientists from the Florida campus of The ... that plays a critical but previously unknown role in ... showed a novel role for a protein known as ... eLife , a publisher supported by the Howard ... Wellcome Trust. , "This is a critical building block ...
(Date:4/22/2014)... nanotechnology expert will present a poster titled ... 7th International Nanotoxicology Congress to be held ... Monita Sharma will outline a strategy consistent with the ... " Toxicity Testing in the 21st Century: A Vision ... methods involving human cells and cell lines for mechanistic ...
(Date:4/22/2014)... Experimental Biology (FASEB) has released updated factsheets ... the National Institutes of Health (NIH) benefits each of ... pleased to make these factsheets available to help citizens ... state," said FASEB President, Margaret K. Offermann, MD, PhD. ... research funding, investing $29.2 billion in FY 2013 in ...
Breaking Biology News(10 mins):Scientists identify critical new protein complex involved in learning and memory 2
... This press release is available in French ... and men contribute equally to the lineage of contemporary populations? ... polygamy or monogamy? To answer these questions, Dr. Damian Labuda, ... a professor at the Department of Pediatrics of the Universit ...
... professor of chemistry at New Jersey Institute of ... patent today for a novel composition of matter. ... (US Patent Number 7,670,684) discloses a new self-contained ... comprised of organic scaffolds with metal centers, which ...
... worldwide. It is the most common form of age-related dementia, ... no cure, and the available drugs only help to relieve ... characteristic changes in the brains of Alzheimer,s patients is the ... curing or at least slowing the disease lies in developing ...
Cached Biology News:Modern man found to be generally monogamous, moderately polygamous 2NJIT researcher awarded patent for hydrophobic, corrosion-resistant coating 2The sea squirt offers hope for Alzheimer's sufferers 2
Candida (IgG) -Ab EIA Sample Size: 5 l...
Candida (IgA) -Ab EIA Sample Size: 5 l...
Automated centrifuge for the processing of microplates and tubes; can be integrated into automated process using inexpensive robotics due to its unique sample handling platform....
... the first higher plant to be fully ... primary model organism for gene expression research ... microarray is derived from the ATH1 v.5 ... (TIGR) and from the Arabidopsis MPSS database ...
Biology Products: