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Ambion Offers Gene Expression Profiling Service


Ambion now offers a complete Gene Expression Profiling Service* for Affymetrix GeneChip Arrays. From basic data analysis to our Full Service offering, no project is too large or too small. The Full Service offering includes consultation with Ambion's team of experienced array and informatics scientists and comprehensive data analysis. Ambion's long history of providing innovative, high quality products and services for use in gene expression profiling insures the highest level of quality.

Two services have been designed to provide high quality, highly reproducible microarray data.


Standard Service

Comprehensive sample Quality Assurance and process Quality Control

Target aRNA preparation (using Ambion's MessageAmp II aRNA Amplification Kit)

Hybridization to Affymetrix GeneChip arrays for expression analysis

Scanning and image analysis

Data packaged and ready for analysis

Process summary report

Turnaround time as short as three days from receipt of sample


Full Service
Includes Standard Service plus

Consultation with Ambion's team of experienced array and informatics scientists

Custom designed experimental and analysis plan

Comprehensive data analyses

Sample processing turnaround time varies by the complexity of the required analyses

Fully licensed to provide services using Affymetrix GeneChip arrays. Fully licensed to provide services using the Eberwine procedure.


Experimental Design (Full Service)

Ambion's Array Services offers much more than what a typical core laboratory can. It is a complete service microarray information group, which aims to maximize the quantity and quality of information that can be obtained from a microarray experiment. The Array Services' staff of experienced biologists, statisticians, and bioinformatics specialists will work with you to organize the most effective and economical approach to accomplish your experimental goals by:

Helping to formulate a concise experimental question

Setting up an experimental design that includes biological and analytical perspectives

Replication for efficient analysis
Sampling
Selection of controls
Cost efficacy: fewest arrays for the most information

Recommending statistical analyses to meet your goals


Target aRNA Preparation

Ambion can prepare aRNA samples starting from both eukaryotic and prokaryotic RNA by using the best linear RNA amplification product on the market: MessageAmp II aRNA Amplification Kit.

Ambion Services uses the MessageAmp II Kit, which allows reproducible amplification from small total RNA samples:

>500 ngone round of amplification

<500 ngtwo rounds of amplification

All amplified samples are then inspected on an Agilent 2100 bioanalyzer to confirm quality (i.e. size and yield).


Hybridization/Scanning

All reagents (hybridization and washes) pass strict quality control standards


Data Analysis
Standard Service

Full report for each array with complete process QC information

Background/Noise Values

Scaling Factor

Percent Present Calls

3'/5' Ratios of Housekeeping Genes


Full Service

Includes Standard Service, plus
Comprehensive Data Analysis:

Data Normalization

Hypothesis Testing

Publication Quality Figures

Clustering

Fully Annotated Gene Lists

Protocols for all procedures are tightly regulated in compliance with ISO standards.

*For more information, customers in the United States and Canada may contact us at arrayservices@ambion.com, and customers served by Ambion (Europe) Ltd. may contact eurotech@ambion.com. Array Services will be available soon in Japan.


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