Navigation Links
Ambion Offers Gene Expression Profiling Service


Ambion now offers a complete Gene Expression Profiling Service* for Affymetrix GeneChip Arrays. From basic data analysis to our Full Service offering, no project is too large or too small. The Full Service offering includes consultation with Ambion's team of experienced array and informatics scientists and comprehensive data analysis. Ambion's long history of providing innovative, high quality products and services for use in gene expression profiling insures the highest level of quality.

Two services have been designed to provide high quality, highly reproducible microarray data.


Standard Service

Comprehensive sample Quality Assurance and process Quality Control

Target aRNA preparation (using Ambion's MessageAmp II aRNA Amplification Kit)

Hybridization to Affymetrix GeneChip arrays for expression analysis

Scanning and image analysis

Data packaged and ready for analysis

Process summary report

Turnaround time as short as three days from receipt of sample


Full Service
Includes Standard Service plus

Consultation with Ambion's team of experienced array and informatics scientists

Custom designed experimental and analysis plan

Comprehensive data analyses

Sample processing turnaround time varies by the complexity of the required analyses

Fully licensed to provide services using Affymetrix GeneChip arrays. Fully licensed to provide services using the Eberwine procedure.


Experimental Design (Full Service)

Ambion's Array Services offers much more than what a typical core laboratory can. It is a complete service microarray information group, which aims to maximize the quantity and quality of information that can be obtained from a microarray experiment. The Array Services' staff of experienced biologists, statisticians, and bioinformatics specialists will work with you to organize the most effective and economical approach to accomplish your experimental goals by:

Helping to formulate a concise experimental question

Setting up an experimental design that includes biological and analytical perspectives

Replication for efficient analysis
Sampling
Selection of controls
Cost efficacy: fewest arrays for the most information

Recommending statistical analyses to meet your goals


Target aRNA Preparation

Ambion can prepare aRNA samples starting from both eukaryotic and prokaryotic RNA by using the best linear RNA amplification product on the market: MessageAmp II aRNA Amplification Kit.

Ambion Services uses the MessageAmp II Kit, which allows reproducible amplification from small total RNA samples:

>500 ngone round of amplification

<500 ngtwo rounds of amplification

All amplified samples are then inspected on an Agilent 2100 bioanalyzer to confirm quality (i.e. size and yield).


Hybridization/Scanning

All reagents (hybridization and washes) pass strict quality control standards


Data Analysis
Standard Service

Full report for each array with complete process QC information

Background/Noise Values

Scaling Factor

Percent Present Calls

3'/5' Ratios of Housekeeping Genes


Full Service

Includes Standard Service, plus
Comprehensive Data Analysis:

Data Normalization

Hypothesis Testing

Publication Quality Figures

Clustering

Fully Annotated Gene Lists

Protocols for all procedures are tightly regulated in compliance with ISO standards.

*For more information, customers in the United States and Canada may contact us at arrayservices@ambion.com, and customers served by Ambion (Europe) Ltd. may contact eurotech@ambion.com. Array Services will be available soon in Japan.


back to top


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Ambion Custom
2. Ambion Custom: Made-to-Order
3. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
4. Your Data: Detection of Oocyte-specific Gene Expression Using Ambions Cells-to-cDNA Kit
5. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
8. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
9. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
10. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
11. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/29/2017)... Mass. , March 29, 2017 /PRNewswire/ ... in applying mechanistic modeling to drug research ... their collaboration with Zymeworks Inc. for quantitative ... conjugate therapeutics intended for the treatment of ... supported Zymeworks previously for GLP toxicology studies ...
(Date:3/29/2017)... ... March 29, 2017 , ... ... for cell-based assays, disperses a quarterly travel award to noteworthy scientists who will ... company announced that its new round of awards are being given to two ...
(Date:3/29/2017)... , Mar 29, 2017 Research and ... Market Analysis & Trends - Industry Forecast to 2025" report ... ... a CAGR of around 7.8% over the next decade to reach ... analyzes the market estimates and forecasts for all the given segments ...
(Date:3/29/2017)... , March 29, 2017 /PRNewswire/ -  GeneNews Limited ... BreastSentry™ , a new risk stratification test for breast ... lab, Innovative Diagnostics Laboratory ("IDL"). BreastSentry incorporates a blood-based biomarker ... and lifetime risk for developing breast cancer.   ... BreastSentry measures the ...
Breaking Biology Technology:
(Date:3/22/2017)... -- Vigilant Solutions , a vehicle location and ... today the appointment of retired FBI special agent ... development. Mr. Sheridan brings more than 21 ... on the aviation transportation sector, to his new role ... served as the Aviation Liaison Agent Coordinator (ALAC) in ...
(Date:3/13/2017)... March 13, 2017 Future of security: Biometric Face Matching ... ... DERMALOGs Face Matching enables to match face pictures against each ... to identify individuals. (PRNewsFoto/Dermalog Identification Systems) ... DERMALOG,s "Face Matching" is the fastest software for biometric Face Matching on ...
(Date:3/7/2017)... , March 7, 2017   HireVue , ... top global companies identify the best talent, faster, today ... Chief Sales Officer (CSO) and Diana Kucer ... round out a seasoned executive team poised to drive continued ... building on a year of record bookings in 2017. ...
Breaking Biology News(10 mins):