Two services have been designed to provide high quality, highly reproducible microarray data.
Comprehensive sample Quality Assurance and process Quality Control
Target aRNA preparation (using Ambion's MessageAmp II aRNA Amplification Kit)
Hybridization to Affymetrix GeneChip arrays for expression analysis
Scanning and image analysis
Data packaged and ready for analysis
Process summary report
Turnaround time as short as three days from receipt of sample
Includes Standard Service plus
Consultation with Ambion's team of experienced array and informatics scientists
Custom designed experimental and analysis plan
Comprehensive data analyses
Sample processing turnaround time varies by the complexity of the required analyses
Fully licensed to provide services using Affymetrix GeneChip arrays. Fully licensed to provide services using the Eberwine procedure.
Experimental Design (Full Service)
Ambion's Array Services offers much more than what a typical core laboratory can. It is a complete service microarray information group, which aims to maximize the quantity and quality of information that can be obtained from a microarray experiment. The Array Services' staff of experienced biologists, statisticians, and bioinformatics specialists will work with you to organize the most effective and economical approach to accomplish your experimental goals by:
Helping to formulate a concise experimental question
Setting up an experimental design that includes biological and analytical perspectives
Replication for efficient analysis
Selection of controls
Cost efficacy: fewest arrays for the most information
Recommending statistical analyses to meet your goals
Target aRNA Preparation
Ambion can prepare aRNA samples starting from both eukaryotic and prokaryotic RNA by using the best linear RNA amplification product on the market: MessageAmp II aRNA Amplification Kit.
Ambion Services uses the MessageAmp II Kit, which allows reproducible amplification from small total RNA samples:
>500 ngone round of amplification
<500 ngtwo rounds of amplification
All amplified samples are then inspected on an Agilent 2100 bioanalyzer to confirm quality (i.e. size and yield).
All reagents (hybridization and washes) pass strict quality control standards
Full report for each array with complete process QC information
Percent Present Calls
3'/5' Ratios of Housekeeping Genes
Includes Standard Service, plus
Comprehensive Data Analysis:
Publication Quality Figures
Fully Annotated Gene Lists
Protocols for all procedures are tightly regulated in compliance with ISO standards.
*For more information, customers in the United States and Canada may contact us at firstname.lastname@example.org, and customers served by Ambion (Europe) Ltd. may contact email@example.com. Array Services will be available soon in Japan.