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Figure 1. Crystals of Group I Intron From Bacteriophage Twort.

Today's laboratory demands often require products and services that fall outside the range of standard kit configurations and sizes from typical life science reagent companies. Specialized protocols may quickly consume reagents in large volumes. For these situations, Ambion's kits, reagents, and enzymes are all available in bulk sizes that can save both time and resources.

Our most popular bulk-sized kits include:

5X MEGAscript High Yield Transcription Kit,
5X MessageAmp II aRNA Kit for RNA amplification for array analysis
5X mMESSAGE mMACHINE High Yield Capped RNA Transcription Kit.

5X MEGAscript Kit Enables Efficient Crystallography

Dr. Barbara Golden's laboratory (Purdue University) used Ambion's 5X MEGAscript High Yield Transcription Kit to transcribe milligram quantities of RNA--3.75 mg purified group I intron from bacteriophage Twort--for crystallography (Figure 1). The superior performance of this kit led to increased product concentrations, which reduced sample volumes and improved the efficiency of subsequent purification steps. Three to five mg/ml RNA generated with this bulk reagent kit allows production of 250-1000 crystallization drops for X-ray crystallography.

To find out how Ambion can help meet your specific research requireme
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