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Figure 1. Crystals of Group I Intron From Bacteriophage Twort.
Today's laboratory demands often require products and services that fall
outside the range of standard kit configurations and sizes from typical
life science reagent companies. Specialized protocols may quickly consume
reagents in large volumes. For these situations, Ambion's kits, reagents,
and enzymes are all available in bulk sizes that can save both time and
resources.
Our most popular bulk-sized kits include:
5X MEGAscript High Yield Transcription Kit,
5X MessageAmp II aRNA Kit for RNA amplification for array
analysis
5X mMESSAGE mMACHINE High Yield Capped RNA Transcription
Kit.
5X MEGAscript Kit Enables Efficient Crystallography
Dr. Barbara Golden's laboratory (Purdue University) used Ambion's 5X MEGAscript High Yield Transcription Kit to transcribe milligram quantities of RNA--3.75 mg purified group I intron from bacteriophage Twort--for crystallography (Figure 1). The superior performance of this kit led to increased product concentrations, which reduced sample volumes and improved the efficiency of subsequent purification steps. Three to five mg/ml RNA generated with this bulk reagent kit allows production of 250-1000 crystallization drops for X-ray crystallography.
To find out how Ambion can help meet your specific research requirements, email us at custom@ambion.com. R&D and production scientists are available to plan and design products specifically for your applications.
Ordering
Information
Cat#
Product Name
Size
B1334-5
5X MEGAscript T7 Kit
200 rxns
B1345-5
5X mMESSAGE mMACHINE
T7 ULTRA Kit
50 rxns
B1751-5
5X MessageAmp II aRNA
Amplification Kit
100 rxns
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