ically
stained and destained,
avoiding manual steps required
with SDS-PAGE.
Direct comparison of samples
The Protein LabChip kits confirm the
expression of a recombinant protein
in cell lysate samples. The software
overlay feature allows identification
of expressed recombinant proteins.
No more gels to dry
The data is automatically stored
in digital format which can be
easily retrieved and shared.
This eliminates the manual
procedures of gel drying
and storage.
Cell assays
Simplified analysis of fluorescence
parameters of single cells
With lab-on-a-chip technology it is
possible to miniaturize assays and
instrumentation used to monitor cell
parameters. The controlled movement
of cells on the chip is generated by
pressure-driven flow.
Agilent offers an add-on pressure
cartridge, cell fluorescence software and
a LabChip kit for multiple types of cell
assay applications. Combined with the
small-footprint Agilent 2100 bioanalyzer,
this makes performing simple flow
cytometric analyses on a chip a reality,
even for the smallest lab.
Cell solutions
The analysis of cell-based attributes is
a daily task in many molecular and cell
biology labs. A simple control method
is crucial for any analysis protocol used
for monitoring the expression of a target
protein, the transfection status of a cell
population, or the time course of an
apoptotic process.
Agilents cell assays can be used to
determine easily a variety of parameters
from many eukaryotic cell lines and
primary cells.
Low cell consumption
As little as 20,000 cells per sample are
loaded on the chip for analysis.
On-chip staining provides the added
benefit of minim
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Monitoring transfection efficiency by
green fluorescent protein (GFP) detection
with the Agilent 2100 bioanalyzer2.
Apoptosis detection by annexin V and
active caspase-3 with the Agilent 2100
bioanalyzer3.
Measuring multiple apoptosis
parameters with the Agilent 2100
bioanalyzer4.
Flow cytometric analysis of human
primary cells using the Agilent 2100
bioanalyzer and on-chip staining5.
Detection of antibody-stained cell
surface and intracellular protein targets
with the Agilent 2100 bioanalyzer6.
2100 expert software
Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer7.
siRNA transfection optimization
with the Agilent 2100 bioanalyzer8.
Quantitative end-point RT-PCR gene
expression measurement using the
Agilent 2100 Bioanalyzer and
standardized RT-PCR9.
Using the Agilent 2100 bioanalyzer for
quality control of protein samples prior to
MS-analysis10.
Rapid detection of genomic
duplications and deletions using
the Agilent 2100 bioanalyzer11.
A Positive Selection Assay for Mutation Analysis in Big Blue
Animals