Automated quality control, sizing and quanitation of RNA, DNA and proteins
Pin-electrodes in the cartridge for molecular assays are used to create electrokinetic forces capable of driving fluids or molecules within fluids through micro-channels to perform electrophoretic separations.
Automated regulation of voltage or current between these electrodes controls the speed and direction of fluid movement. The miniaturized fluid pathways shorten run time, and the strong electrokinetic driving forces improve analyte resolution.
Agilent offers a wide range of LabChip kits for the analysis of RNA, DNA and proteins. In conjunction with the Agilent 2100 bioanalyzer, equipped with electrode cartridge, these solutions have many advantages over traditional slab gel analysis:
Reduced manual labor
Fast, accurate and reproducible results
Automated data acquisition and analysis
Digital data for easy archiving
Minimized exposure to hazardous material and reduced waste volume
Agilent offers RNA assays that allow characterization of total or mRNA samples fast, with unmatched sensitivity and minimal sample consumption. The RNA 6000 Nano LabChip kit is the industry standard for quality control and quantitation of total and mRNA samples for microarray experiments. The RNA 6000 Pico kit delivers the highest sensitivity for RNA quality control to date, making it the tool of choice for the analysis of RNA from microdissected samples, needle biopsies and first strand cDNA.
High sensitivity with small sample volume As little as 200 pg/l RNA is required per analysis. This saves you sample for any following experiments.
Catch RNA degradation right away RNase degradation of total RNA is easily detected by a shift in the RNA size distribution towards smaller fragments and a decrease in fluorescence signal of ribosomal peaks.
Automated detection of ribosomal RNA contamination The Agilent 2100 bioanalyzer software integrates the messenger RNA smear and calculates the percentage of ribosomal RNA contamination.
Optimization of PCR reactions for sequencing, cloning and typing Agilent's DNA assays allow you to automatically size and quantitate PCR fragments and restriction digests accurately and reproducibly. PCR and RT-PCR are among the most widely used techniques in molecular biology. While in some cases, it is sufficient to detect the presence or absence of a PCR product, in many cases quantitation of this product and detection of unspecific amplification is critical. The Agilent 2100 bioanalyzer is the tool of choice for automated sizing and quantitation of PCR and RT-PCR products in single or multiplex mode with unprecedented accuracy.
High sensitivity Higher sensitivity, broad linear dynamic range and sharpness of bands enables the detection of less abundant products that are not visible in an agarose gel (e.g. non-specific amplifications or carry-over products). Improved sizing accuracy Normalization to two internal markers and a ladder provides accurate and reproducible sizing. Automated and reproducible quantitation The Agilent 2100 bioanalyzer software quantitates each DNA fragment automatically against internal standards. This ensures increased accuracy and reproducibility.
Ensure successful protein purification along each step
Agilents protein assays can be used to analyze cell lysates, column fractions and purified proteins 10 samples in less than 30 minutes.
Purifying a protein from crude extracts or whole cell lysates is tedious and involves many steps along which the protein of interest can be lost. The 2100 bioanalyzer and Protein LabChip kits monitor the presence of a protein during each purification step, faster and less laborious than with conventional SDS-PAGE. Be in control Rapidly check the success of your purification procedure during every step of the process.
Significant time saving From the time you press 'START' see your first results after 7 minutes and subsequent results at 2-minute intervals. No need for staining/destaining During the run, samples are automatically stained and destained, avoiding manual steps required with SDS-PAGE. Direct comparison of samples The Protein LabChip kits confirm the expression of a recombinant protein in cell lysate samples. The software overlay feature allows identification of expressed recombinant proteins.
No more gels to dry The data is automatically stored in digital format which can be easily retrieved and shared. This eliminates the manual procedures of gel drying and storage.
Simplified analysis of fluorescence parameters of single cells
With lab-on-a-chip technology it is possible to miniaturize assays and instrumentation used to monitor cell parameters. The controlled movement of cells on the chip is generated by pressure-driven flow. Agilent offers an add-on pressure cartridge, cell fluorescence software and a LabChip kit for multiple types of cell assay applications. Combined with the small-footprint Agilent 2100 bioanalyzer, this makes performing simple flow cytometric analyses on a chip a reality, even for the smallest lab.
The analysis of cell-based attributes is a daily task in many molecular and cell biology labs. A simple control method is crucial for any analysis protocol used for monitoring the expression of a target protein, the transfection status of a cell population, or the time course of an apoptotic process.
Agilents cell assays can be used to determine easily a variety of parameters from many eukaryotic cell lines and primary cells. Low cell consumption As little as 20,000 cells per sample are loaded on the chip for analysis. On-chip staining provides the added benefit of minimal reagent and cell consumption. Ease of use Extensively documented pre-defined applications allow you to start right away. These examples include detection of antibody binding, apoptosis monitoring or detection of green fluorescent protein. Flexibility The possibility to establish customdesigned assays with the help of easy software tools broadens the applicability of the system.
The software for molecular assays and the software for cell assays provide easy instrument control, access to real-time data, automated data analysis and valuable tools for further data evaluation. Free data review software for off-line evaluation allows you to share data with other scientists all over the world.
The data organizer software is a useful complement to the system software, providing quick access to centrally stored raw data files and analysis results for review, re-evaluation or export.