The Polymerase Chain Reaction invented by Kary Mullis in 1983 drastically changed the course of modern life science research by opening an enormous variety of new opportunities to study and understand the crucial role of nucleic acids in living organisms. Roche Applied Science recognized early-on the importance of this new technology and the necessity to have a thermostable enzyme available allowing the scientific community to exploit the Polymerase Chain Reaction. Since then, Roche Applied Science has strived to continuously perfect the Polymerase Chain Reaction. By exploiting their traditional experience in producing biochemicals and enzymes, RAS has developed and perfected a broad range of new associated technologies and more powerful enzyme preparations. Today, these highly optimized, innovative preparations enable scientists to obtain performance beyond the standard in specificity, sensitivity, accuracy and reproducibility. Roche Applied Science has a strict set of guidelines based upon its overall quality philosophy:

• All amplification reagents have without any compromises the highest possible purity
• All amplification reagents are rigorously function tested using the most stringent quality control criteria
• All amplification reagents are produced using state-of-the-art facilities and technologies
• All available in-house knowledge is used to assure the availability of new, innovative products and systems
• Establish partnerships with our customers to develop new ideas into products
• Actively use our worldwide distribution channels to deliver our products in the best possible terms and conditions to our customers
• Provide in-depth product and application information to our customers

The results of the above policy are clear: Many customers rely on our products and services on a daily basis and have learned to expect a consistent high quality of our amplification products. Both internal results and co-operations with various customers resulted in a constant stream of innovative product enabling the scientific community to achieve new milestones that were previously not possible:

• 1990: Introduction of Taq DNA Polymerase
  
  
• 1995: Introduction of the PCR Elisa Technology the first system enabling the non-radioactive detection of PCR products in a microplate format
  
  
• 1995: Introduction of the Expand product family the first blend of thermostable enzymes allowing the amplification of amplicons up to 10 kb
  
  
• 1996: Introduction of the Titan product family until today the most sensitive system for detecting rare messages
  
  
• 1997: Introduction of a ultrapure PCR Grade Nucleotides based upon a new manufacturing process developed at Roche Applied Science
  
  
• 1998: Introduction of the C. therm product family the most robust system to amplify mRNA templates with complex secondary structures
  
  
• 1998: Introduction of the GC RICH PCR System another blend of enzymes powering the amplification of GC RICH templates or templates with high repetitive sequences that could not be amplified with "conventional" polymerases or additives
  
  
• 1998: Introduction of the LightCycler the first system allowing the real-time on-line monitoring of an amplification reaction
  
  
• 1999 and 2000: Introduction of various reagents supporting the use of the LightCycler System for a wide variety of applications
  
  
• 2000: Introduction of FastStart Taq DNA Polymerase - the next-generation PCR system for hot start applications
  
  
• 2002: Introduction of Expand High Fidelity^PLUS PCR System the first product of a family of next-generation PCR Blends
  
  

Roche Applied Science continues to believe that the potential of this technology is not fully exploited yet and is strongly committed to continue serving the scientific community with new innovative systems and reagents without any compromises on its original quality philosophy.
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