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Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

Comparative Differences
To run the handcast gel, 2 L of 1x TAE was required, while only 1.2 L of the same buffer was needed to run the two ReadyAgarose gels

It took 45 min to run the ReadyAgarose gels at 100 V, while it took 90 min to run the handcast gel at the same voltage

When the gels were imaged, the background of the handcast gel was not uniform, suggesting that the ethidium bromide was not distributed uniformly, probably because of the high viscosity of a 3% agarose solution (Figure 1). However, no such unevenness was seen in the ReadyAgarose gels (Figure 2)

With the numbered wells on the ReadyAgarose gel tray, documentation of lane samples was both easier and less prone to error. The ruler on the ReadyAgarose tray also facilitated easy and accurate measurement of band migration distances

Time and Cost Differences
The total time needed to prepare and run the handcast and precast gels is compared in Figure 3. A sample cost analysis for 3% gels is presented in Table 1.


Conclusions
In our tests, both handcast and precast gels gave equivalent band sharpness for PCR samples. However, the use of two 2 x 32-well ReadyAgarose precast gels instead of a large 96-sample (102-well) handcast gel saves preparation and running time. Two ReadyAgarose 2 x 32-well gels offer more lanes than a handcast gel designed specifically for 96 samples, thus allowing extra samples or standards to be included in a single run. Data from ReadyAgarose gels are easier to document since well numbers and a ruler are printed on the tray. These advantages of using two ReadyAgarose gels over one large
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