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Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

Divya Sharma1 and Carolyn Seggerson2, 1Tech/Aid, 7700 Edgewater Drive, Oakland, CA 94621 USA, and 2Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA


Introduction
Running PCR samples on agarose gels is a routine yet timeconsuming procedure. It is common to hand cast a horizontal 96-sample gel for this purpose. A gel of such large size is difficult to cast, handle, and image, and requires a long run time. These issues can be resolved by using two of Bio-Rads 2 x 32-well ReadyAgarose precast gels instead. ReadyAgarose gels are cast in their own UV-transparent running tray with fluorescent well numbers and a ruler for easy reference. Their gel trays have autolock tabs that snap into the dedicated wide mini ReadySub-Cell GT cell, so you can simply drop one of these precast gels into place and load your samples. ReadyAgarose gels fit most non-Bio-Rad wide mini subcells as well and thus do not require equipment from a specific manufacturer. ReadyAgarose gels are available in 0.8%, 1%, and 3% agarose in TBE or TAE buffer, with or without ethidium bromide, to fit your research requirements. In addition, two 2 x 32-well ReadyAgarose gels offer more wells than a 96-sample handcast gel, run in 3045 min, and have 4.0 cm of resolving distance. These precast gels are designed to give the same results as traditional handcast gels in a fraction of the time.


Methods
Hand Casting a 96-Sample Gel
A gel was made by adding 8.7 g of Bio-Rad Certified low range ultra agarose (the same agarose used to manufacture 3% ReadyAgarose gels) to 290 ml of 1x TAE buffer and heating
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