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Advancing the quality control methodology,,,to assess isolated total RNA,,,and generated fragmented cRNA

the well, improperly securing the gel to the gel rig, not running the gel on the appropriate voltage/time or not staining the gel properly. These errors are reflected in the gel images below (Figures 1-6). These problems would be eliminated using the Agilent 2100 bioanalyzer.





MOPS gels may be suitable to get an idea of the quality of samples, but the gels do not always clearly reveal a detailed image of the sample. It is important that the genomics information GeneLogic, Inc. provides to its customers is of the highest quality. Therefore, there needs to be an accurate, reliable way of assessing the quality of the samples.

Agilent 2100 bioanalyzer vs MOPS gels for Total RNA
The Agilent 2100 bioanalyzer was tested as a tool for quality control for total RNA and compared against the MOPS gels for the same samples over a series of weeks. During this time a grading system was derived for the sample data using the Eukaryote Total RNA Nano assay with the bioanalyzer. It was determined that the samples were either given a pass, fail, or Pass needs additional QC rating. Pass-Needs additional QC would essentially mean that the sample was passed, but that it would from then on be closely monitored for its quality in further steps. Additional Quality Control assays would be employed to further evaluate the quality of these samples. For the MOPS gels a system was already in place to either pass or fail a sample. Because of the clarity of the electropherograms produced using the bioanalyzer, distinguishing between the high quality samples and poor quality samples became easier. For example, when samples were analyzed using b
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