Advancing the quality control methodology,,,to assess isolated total RNA,,,and generated fragmented cRNA
well, improperly securing the gel
to the gel rig, not running the gel
on the appropriate voltage/time or
not staining the gel properly.
These errors are reflected in the
gel images below (Figures 1-6).
These problems would be eliminated
using the Agilent 2100 bioanalyzer.
MOPS gels may be suitable to get
an idea of the quality of samples,
but the gels do not always clearly
reveal a detailed image of the sample.
It is important that the
genomics information GeneLogic,
Inc. provides to its customers is of
the highest quality. Therefore,
there needs to be an accurate, reliable
way of assessing the quality
of the samples.
Agilent 2100 bioanalyzer vs MOPS
gels for Total RNA
The Agilent 2100 bioanalyzer was
tested as a tool for quality control
for total RNA and compared
against the MOPS gels for the
same samples over a series of
weeks. During this time a grading
system was derived for the sample
data using the Eukaryote Total
RNA Nano assay with the bioanalyzer.
It was determined that the
samples were either given a
pass, fail, or Pass needs
additional QC rating. Pass-Needs
additional QC would essentially mean that the sample was
passed, but that it would from
then on be closely monitored
for its quality in further steps.
Additional Quality Control
assays would be employed to
further evaluate the quality of
these samples. For the MOPS
gels a system was already in
place to either pass or fail a
sample. Because of the clarity
of the electropherograms produced
using the bioanalyzer,
distinguishing between the
high quality samples and poor
quality samples became easier.
For example, when samples
were analyzed using b
Source:Page: All 1 2 3 4 5 6 Related biology technology :1
. Using the Agilent 2100 bioanalyzer for
quality control of protein samples prior to
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. Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples4
. Quality control of antibodies using the
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. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases6
. Easy preparation of total RNA from cultured cells with TRIzol and
Eppendorf Phase Lock Gel
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