Navigation Links
Advancing the quality control methodology,,,to assess isolated total RNA,,,and generated fragmented cRNA

at times the 18S and/or 28S bands of the total RNA were not clearly visible on the gels making the interpretation difficult. When running fragmented cRNA samples on MOPS gels it was often difficult to distinguish whether or not the samples were allowed a sufficient amount of time to fragment because of poor resolution. Problems with MOPS gels would lead to re-running of the samples on new gels, therefore wasting valuable time and money. The samples that should have failed would be caught by quality control measures later on in the process, but would ultimately cost the company money on reagents in the steps leading up to that.
The Agilent 2100 bioanalyzer combined with the RNA 6000 Nano LabChip Kit provides key advantages to running MOPS gels. The Agilent bioanalyzer data is easy to interpret and the chip runs on the bioanalyzer are less time consuming. It can take up to an hour and half to prepare the samples, run a gel, stain the gel, and take a picture. The whole Agilent process (sample preparation to saving the file) can be done in half that time. There is no improper staining and no shortened or lengthened run times. Also, because each chip has the capacity to run 12 samples, at approximately 30 minutes per chip, this makes the Agilent 2100 bioanalyzer a useful tool for high throughput screenings, especially with multiple bioanalyzers.
The goal was to find a new assay to be used to evaluate the quality of the total RNA and fragmented cRNA. This was needed because there was occasional inconsistency with the use of MOPS gels. The qualitative analyses should be as definitive and dependable as possible. Problems associated with MOPS gels in a high throughput environment may be due to user error. Common mistakes seen in the laboratory are: accidentally piercing the gel while loading, not loading enough of sample into
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
2. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
3. Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples
4. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
Post Your Comments:
(Date:8/20/2014)... 2014  PAREXEL International Corporation (NASDAQ: PRXL ... the Baird Healthcare Conference in New York.  Ingo ... will be making a presentation on PAREXEL and discussing ... 3, 2014. A live webcast of the ... PAREXEL,s website at www.PAREXEL.com in the Upcoming ...
(Date:8/20/2014)... August 20, 2014 Not long ago, ... studies should be performed late in the development cycle ... But current regulatory trends suggest that, like many things ... rise. In recent years, multiple drug sponsors have been ... address specific questions during phase I/II. It’s not only ...
(Date:8/20/2014)... nerves in your ear could improve the health of ... the University of Leeds used a standard TENS machine ... electrical pulses to the tragus, the small raised flap ... of the ear canal. , The stimulation changed the ... reducing the nervous signals that can drive failing hearts ...
(Date:8/20/2014)... GraphDB™ 6.0 from ... the enterprise replication cluster, faster loading speeds, higher ... Elasticsearch. This release happens to coincide with a ... GraphDB™ was formerly known as OWLIM. ... enterprise resilient RDF triplestore will also benefit from ...
Breaking Biology Technology:PAREXEL International To Present At Baird Healthcare Conference 2Are Extractable and Leachables going Phase-Appropriate? New Webinar Hosted by Xtalks 2'Tickling' your ear could be good for your heart 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4
... WALTHAM, Mass. , June 2 Repligen Corporation (Nasdaq: RGEN ... quarter and fiscal year 2010, on Wednesday, June 9th, 2010 , before ... Herlihy , Ph.D., will host a conference call and webcast on the same ... provide a quarterly update on the Company. , ...
... June 2 Vermillion, Inc. (Pink Sheets: VRML), ... Patent Office has issued a Decision to Grant patent ... Cancer" to the Company. The patent claims are directed ... ovarian cancer and to the measurement of the biomarkers ...
... ... The iuvo™ Chemotaxis Assay ( http://www.bellbrooklabs.com/iuvo_chemotaxis_assay_plate.html ) Plate ... measurements of neutrophil chemotaxis ( http://www.bellbrooklabs.com/iuvo_chemotaxis_assay_plate.html ) in a ... time period of the assay. Designed for use ...
Cached Biology Technology:Repligen Announces Conference Call of Fourth Quarter and Fiscal Year 2010 Financial Results 2Repligen Announces Conference Call of Fourth Quarter and Fiscal Year 2010 Financial Results 3Vermillion Announces Issuance of European Patent 2Vermillion Announces Issuance of European Patent 3BellBrook Labs Launches Groundbreaking New Microplate for High Content Chemotaxis Assays in a Stable Gradient 2BellBrook Labs Launches Groundbreaking New Microplate for High Content Chemotaxis Assays in a Stable Gradient 3BellBrook Labs Launches Groundbreaking New Microplate for High Content Chemotaxis Assays in a Stable Gradient 4
(Date:8/20/2014)... Carolina State University researchers have developed methods for electronically ... the electrical signals moths use to control those muscles. ... remotely-controlled moths, or "biobots," for use in emergency response. ... whether we can control the movement of moths for ... says Dr. Alper Bozkurt, an assistant professor of electrical ...
(Date:8/19/2014)... be made to decrease China,s increasing caesarean section rate, ... BJOG: An International Journal of Obstetrics and Gynaecology ... caesarean delivery rates in the world. Of 16 million ... Although the exact rate is not known, the current ... total caesarean rates ranging from 36% to 58%. However, ...
(Date:8/19/2014)... Long-Term Ecological Research and Network-Level Science, , Imagine if ... range of ecosystems was responding to global changes in ... if it coupled multiple decades of information about ecological ... models from dozens of different ecosystem types. , ... Ecological Research (LTER) Network, which will soon celebrate its ...
Breaking Biology News(10 mins):Research paves way for development of cyborg moth 'biobots' 2Is China's 50 percent cesarean section delivery rate too high? 2Is China's 50 percent cesarean section delivery rate too high? 3This week from AGU: Long-term ecological research, predicting cholera outbreaks 2
... Not all trans fats are created equal and it,s time ... reflect this, particularly when it comes to dairy and beef ... the latest edition of Advances in Nutrition , natural ... beef cattle are not detrimental to health and in fact ...
... team of leading marine scientists from around the world ... the deep sea, the Earth,s largest ecosystem. Instead, ... consumers., In a comprehensive analysis published online this week ... fisheries biologists, economists, mathematicians and international policy experts show ...
... fared better than other non-advanced technology exports during the recent ... Foundation (NSF). NSF,s National Center for Science and Engineering ... 2008 to $245 billion in 2009. But this 9 ... ATP exports. ATP exports embody new or leading edge ...
Cached Biology News:Time to reboot thinking on trans fats -- natural trans fats from dairy and beef are good 2Deep-sea fish in deep trouble 2Deep-sea fish in deep trouble 3Recent trends show recession's effect on US advanced technology exports 2
... produced by a patented microwave process which ... consistent, even cell attachment, increased cell growth ... growth uunder difficult conditions, including reduced serum ... a more ecominic alternative to expensive and ...
...
Mouse monoclonal antibody raised against a partial recombinant ARHGEF11. NCBI Entrez Gene ID = ARHGEF11...
... Lines ,High Quality, Functionally-Validated, Ion Channel Cell ... for having a critical role in nerve ... key function in pain, CNS and the ... been investigated in therapeutic areas, such as ...
Biology Products: