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Actinobacillus pleuropneumoniae

o the device.
3. Electroporation:

Mode Prokaryotes O Voltage (V) 1,250 V Time constant (T) 5 ms
4. Immediately add 1 ml outgrowth medium and incubate for 3 h.
5. Plate onto selective Columbia agar plates.

Expected results:

Transformation efficiency up to 1 x 107 transformants/g of DNA.


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