Acrylamide Polymerization A Practical Approach ( lymerization visible gelation occurs i...)

Acrylamide Polymerization A Practical Approach

lymerization, visible gelation occurs in 1520 min and polymerization is essentially complete in 90 min. In photochemical polymerization, however, visible gelation takes 3060 min and complete polymerization requires up to 8 hr (Righetti et al. 1981). Shorter times lead to more porous and elastic gels, increased risk of protein modification, and pore size irreproducibility.

Temperature
Temperature control is critical for reproducibility of acrylamide polymerization. Temperature has a direct effect on the rate of gel polymerization; the polymerization reaction is also exothermic. Consequently, the generated heat drives the reaction more quickly. Thus, gelation usually occurs very rapidly once polymerization begins.

Temperature also affects the properties of the gel (Chen and Chrambach 1979). For example, polymerization at 04C results in turbid, porous, inelastic gels, and reproducibility is difficult to achieve. These properties may be due to increased hydrogen bonding of monomer at low temperatures. Gels polymerized at 25C are more transparent, less porous, and more elastic. However, if the polymerization temperature is too high, short polymer chains are formed and the gels are inelastic. This is thought to be due to increased polymer chain termination at higher temperatures.

A temperature of 2325C is optimal (as well as most convenient) for polymerization. It is important that the monomer solution and the gel mold (e.g., glass plates or tubes) be at the optimal temperature when the gel is poured. Furthermore, reproducibility is dependent on using the same temperature each time gels are poured.

Since monomer solutions are usually stored at 4C along with buffer
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