Navigation Links
Acrylamide Polymerization A Practical Approach

ochemical polymerization is initiated by riboflavin (or riboflavin-5'-phosphate), or by a combination of riboflavin and ammonium persulfate. Initiation and polymerization are catalyzed by TEMED. Because polymerization is initiated by the generation of free radicals from persulfate or riboflavin, it is not surprising that these compounds are reactive, and prone to oxidation or decomposition. The contaminants of the initiators tend to be the products of their own breakdown as well as other contaminating compounds.

TEMED is subject to oxidation, which causes the gradual loss of catalytic activity. This process is greatly accelerated by contaminating oxidizing agents. TEMED that contains oxidation products is characterized by a yellow color. The practical consequences of the oxidative process are the requirement for greater amounts of TEMED to achieve adequate polymerization, and a gradual loss of TEMED reactivity with time. TEMED is also very hygroscopic and will gradually accumulate water, which will accelerate oxidative decomposition. TEMED with maximum activity and shelf life is obtained by redistillation immediately prior to bottling, resulting in a product that is clear, water free, and greater than 99% pure (14.4 M).

Ammonium persulfate is also very hygroscopic. This property is particularly important, since ammonium persulfate begins to break down almost immediately when dissolved in water. Therefore, the accumulation of water in ammonium persulfate results in a rapid loss of reactivity. This is why ammonium persulfate solutions should be prepared fresh daily. Persulfate is consumed in the polymerization reaction. Excess persulfate can cause oxidation of proteins and nucleic ac
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS
2. Practical Applications of HyperDSC in a Pharmaceutical Laboratory
3. Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography
4. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
5. Simple Approaches for Optimization of RT-PCR
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Acrylamide Polymerization Practical Approach

(Date:9/3/2015)... SAN DIEGO , Sept. 3, 2015 /PRNewswire/ ... factors, and a recent spinout of Stemedica Cell ... Thomas H. Silberg as CEO of StemProtein. ... technology for producing and preserving Stem Cell Factors ... is a seasoned professional in the pharmaceutical and biopharmaceutical ...
(Date:9/2/2015)... -- Axovant Sciences Ltd. (NYSE:  AXON), a leading clinical-stage biopharmaceutical ... presentations at three investor conferences: , Wednesday, September ... in New York at the New ... PM at the BioCentury NewsMakers Conference in New ... Center , Thursday, September 17 at 9:30 AM at ...
(Date:9/2/2015)... ... September 02, 2015 , ... The wet form of macular ... anti-vascular endothelial growth factor agents given by injection into the eye. Dry macular ... scientific protocol for the many millions diagnosed with it. AMD usually starts in ...
(Date:9/2/2015)... Mass. (PRWEB) , ... September 02, 2015 , ... ... includes development of botanical and plant-derived compounds. BCC Research reveals in its ... past 2020 because this market defines the category and generates the most market ...
Breaking Biology Technology:StemProtein Names Thomas H. Silberg As CEO, To Focus On Stem Cell Secreted Proteins Addresing A Variety Of Health And Beauty Related Needs 2StemProtein Names Thomas H. Silberg As CEO, To Focus On Stem Cell Secreted Proteins Addresing A Variety Of Health And Beauty Related Needs 3Axovant Sciences To Present At Upcoming Investor Conferences 2Axovant Sciences To Present At Upcoming Investor Conferences 3StemCell GeneticMed Announces That It Has Started Stem Cell Treatments for Age Related Dry Macular Degeneration (AMG) 2Plant-derived Compounds Poised to Assume Greater Role in Global Drug Markets, According to BCC Research 2Plant-derived Compounds Poised to Assume Greater Role in Global Drug Markets, According to BCC Research 3
... Category Winners and Names Ticket Software LLC as ... 6 The Connecticut Technology,Council and the accounting ... companies in Connecticut last night at the Chevrolet ... had been released last,month and included such well ...
... N.J., Nov. 6 Memory Pharmaceuticals,Corp. (Nasdaq: MEMY ... Head of Business Development, will provide a company update ... Tuesday,November 11, 2008 at 11:10 a.m. EST at the ... parties may access the live webcast of the presentation ...
... Inc. (NASDAQ:,ONTY) (TSX: ONY) (the "Company") today reported financial ... 2008., Total revenue was $0.8 million and $4.0 ... 30, 2008, compared to $1.1 million and $1.9,million for ... revenue,for the nine month period reflects increased sales of ...
Cached Biology Technology:Connecticut Technology Council Honors UHY LLP Tech Top 40 Winners 2Connecticut Technology Council Honors UHY LLP Tech Top 40 Winners 3Connecticut Technology Council Honors UHY LLP Tech Top 40 Winners 4Oncothyreon reports third quarter 2008 financial results 2Oncothyreon reports third quarter 2008 financial results 3Oncothyreon reports third quarter 2008 financial results 4Oncothyreon reports third quarter 2008 financial results 5
(Date:8/31/2015)... Canada , August 31, 2015 ... of biometrics technology in government digitization projects to drive ... to a recently published TechSci Research report, " India Biometrics ... India is projected to grow at a ... the market is anticipated on account of extensive use of ...
(Date:8/25/2015)... SALT LAKE CITY and BELLEVUE, ... a global leader in advanced robotic systems, announced today ... first-of-its-kind robotic unmanned ground vehicle (UGV), at the National ... Lake City.  The Guardian S is ... is the culmination of years of research and in-field ...
(Date:8/20/2015)... 2015   Sensory, Inc ., a Silicon ... experience and security of consumer electronics through state-of-the-art ... its TrulySecure™ is the first multimodal ... FIDO Certified™. The FIDO (Fast Identification ... FIDO UAF (Universal Authentication Framework) 1.0 specifications, which ...
Breaking Biology News(10 mins):India Biometrics Market to Grow at Over 35% Through 2020, Says TechSci Research 2India Biometrics Market to Grow at Over 35% Through 2020, Says TechSci Research 3Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 2TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 4TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 5
... TUCSON, AZCapillary mats are popular in the retail nursery ... mats provide automated irrigation to a variety of plants, ... Made with absorbent fabric lined with polyethylene film ... polyethylene or similar material, the mats help minimize evaporation ...
... of a shorebird called the red knot have been linked ... red knots showed that the average weight of red knots ... primary food source, eggs of horseshoe crabs, has been reduced. ... to departure weight, and that the population size of red ...
... a new, year-long University of Pennsylvania survey of dog ... aggressive pets, veterinary researchers have found that most of ... techniques are modified. The study, published in the ... also showed that using non-aversive or neutral training methods ...
Cached Biology News:Capillary mats labor-saving, economical alternative to hand watering 2Decline of shorebird linked to bait use of horseshoe crabs 2If you're aggressive, your dog will be too, says veterinary study at University of Pennsylvania 2
HistoScan AP Universal Goat Kit...
Interact with both DNA and RNA; fluorescence green (Em=525 nm) bound to DNA; and fluorescence red (Em=~650 nm) bound to RNA....
One bottle containing 30 ml of protein solution and 0.1% sodium azide....
...
Biology Products: