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Acrylamide Polymerization A Practical Approach

pends upon the relative mobility of the proteins used as standards.

Although the y-intercept value will be different for every gel acrylamide percentage, and slightly different for every set of standards, the value should be highly reproducible from gel to gel if the same acrylamide percentage and standards are used. Thus, monitoring the y-intercept of the log molecular weight vs. relative mobility plot is an excellent assessment of reproducibility in polymerization technique.

* %C = (grams crosslinker x 100)/(grams monomer + grams crosslinker)


Handling of Acrylamide
Use good laboratory practices, work in a well ventilated area and wear proper personnel proctective equipment. Refer to the MSDS for further information.

Reagent Storage and Shelf Life
Acrylamide and bis-acrylamide Electrophoresis-purity acrylamide and bis can be stored dry at room temperature (2325C) for at least 1 year.

Ammonium persulfate and potassium persulfate These initiators can be stored tightly sealed at room temperature for at least 1 year. Solutions should be made fresh daily, since persulfate in solution decomposes rapidly. Persulfate is a strong oxidizing agent. Disposal should be in accordance with local regulations.

TEMED This initiator can be stored tightly closed either at 4C or at room temperature for at least 6 months.

After 10 to 12 months, a significant reduction in reactivity requires an increase in the concentration required for proper polymerization. This loss of reactivity is probably due, at least in part, to the gradual accumulation of water.

Riboflavin and riboflavin-5'-phosphate These photoinitiators can be s
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