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Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard

uted to the autosampler operation.

Autosampler Evaluation Various modes of autosampler operation are tested using the DS. These operational modes include full loop, partial loop, and variations of the loop-filling methods. The influence of temperature and other physical parameters are also investigated.

Schematics
A second sampling valve is inserted between the HPLC pump and the sampling valve. (Figure 1). The added valve is used to inject the DS. The complete setup includes, in addition to the standard equipment, a second sampling valve, a DS solution reservoir, and a peristaltic pump to fill the DS sampling loop (Figure 2). The operation of the peristaltic pump and the DS sampling valve are synchronized with the autosampler operation through the autosampler time functions.

Peristaltic Pump: Ismatec , 4 channel, 8 roller, 115/220V, Distributed by Cole-Parmer Instrument Co. Part #78017-10

Automated Sampling Valve : Rheodyne EV700-100-SU Valve, 6 port, 2 position, Stainless Steel Valve, PEEK Rotor, Distributed by Sigma-Aldrich, Supelpro Part #53148-U

Procedure
For this experiment the peristaltic pump is turned on at the beginning of the run (time 0) with the DS sampling valve in the Load position. At 5.10 minutes the DS sampling valve is switched to the Inject position. The starting of the peristaltic pump and the DS injection time are a function of the application. The duration of the pumping should be long enough to ensure complete filling of the DS sampling loop. It takes 10 times the loop volume to have it filled completely to 99.9%. This provides for the best reproducibility. The DS should be injected at a time that ensures proper elution without interference from other peaks.

Result s
The DS as a Diagnostic Tool
Figure 3 demonstrate
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