Navigation Links
ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S.
Molecular Devices Corporation, 8/00


INTRODUCTION
Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell number.ATP measurements are used to monitor raw materials and manufacturing planthygiene for bacterial contamination in the food, drug, health care and personalhealthcare industries, as well as for waste water analysis.1 In the biotechnologyand pharmaceutical industries, ATP measurements are used to evaluate cellproliferation and cytotoxicity. The bioluminescent assay of ATP has become verypopular because of its high sensitivity and convenience. In this application note,we show the ATP assay results obtained with the Molecular Devices Lmaxmicroplate luminometer.


PRINCIPLE OF
ASSAY
The basis for the assay is the reaction catalyzed by luciferase, obtained from the common American firefly (Photinus pyralis).2 The enzyme catalyzes the ATP-dependent oxidation of luciferin with the concomitant release of light (Figure 1).When ATP is the limiting component in the reaction, the amount of light emittedis proportional to the concentration of ATP. The intensity of the emitted light canbe measured easily with the Lmax microplate luminometer.


EQUIPMENT AND MATERIALS
1. Lmax microplate luminometer with SOFTmax PRO for Lmax (Molecular Devices Corp.)

2. ENLITENTM ATP Assay System, Cat. No. FF2000; Promega Corporation. 608277-2516. The kit contains Luciferase/Luciferin (L/L) reagent, ATP standard 10-7 M in HEPES buffer, pH 7.75) and ATP-free water.

3. Solid white 96-well microplates; e.g. Porvair PS white, E&G Wallac Cat. # 204003; Tel: 1-800-221-9650; or CorningCostar Cat. No.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:1/22/2015)... Boulder, CO / Rootstown, OH (PRWEB) January 22, 2015 ... sensitive detection platform, announced today that it has received AOAC-PTM ... O26, O45, O1O3, O111, O121, and O145; collectively referred to ... (E. coli) O157, at 1 colony forming unit (cfu) per ...
(Date:1/22/2015)... 22, 2015 Selexis SA, a serial ... Cell Banks (RCBs) used for drug discovery to commercial ... will include Next-Generation Sequencing (NGS) data packages. ... biologic manufacturing by ensuring the integrity of the gene, ...
(Date:1/22/2015)... 2015   GenoSpace , a precision medicine software company that ... broad use of genomic, imaging and other biomedical data in ... Michelle Munson , CEO of Aspera, an IBM Company, to ... http://photos.prnewswire.com/prnh/20150122/170713 "We are pleased to ...
(Date:12/24/2014)... 24, 2014  Vermillion, Inc. (Nasdaq:   VRML), a ... today that the Company closed its previously announced ... Jack W. Schuler , Birchview Fund LLC ... million, before offering expenses.  The proceeds will be ...
Breaking Biology Technology:Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2GenoSpace Expands Board with Appointment of Michelle Munson 2Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3
... Ltd. (Nasdaq: XTLB ; TASE: XTL) announces ... Meeting ("EGM") scheduled,for today were adjourned and postponed ... pm Israel time, respectively., ABOUT XTL BIOPHARMACEUTICALS ... in the development of,therapeutics for the treatment of ...
... Product Quickly Approaches World-wide Availability, FREDERICK, ... BIEL) announces today a new distribution,agreement with ... distribution of,BioElectronic,s revolutionary ActiPatch product., Piramal ... is,one of India,s largest companies with strong ...
... Will Focus on Strategies to Promote Legislative Pathway for ... ... (Nasdaq:,INSM), a developer of follow-on biologics and biopharmaceuticals for unmet,medical needs, ... Ways and Means Committee from 2001 to 2007, has been,retained by ...
Cached Biology Technology:BioElectronics Announces Distribution Agreement for Sales In India 2BioElectronics Announces Distribution Agreement for Sales In India 3Insmed Partners With Bill Thomas, Former House Ways and Means Chairman, as Strategic Advisor 2Insmed Partners With Bill Thomas, Former House Ways and Means Chairman, as Strategic Advisor 3Insmed Partners With Bill Thomas, Former House Ways and Means Chairman, as Strategic Advisor 4
(Date:12/17/2014)... Dec. 16, 2014 Valencell, a leader in performance ... technology to industry leaders such as Intel, Jabra, Atlas, ... biometric wearable products. These products will be showcased at ... Las Vegas . "Our partners ...
(Date:12/15/2014)... , Dec. 12, 2014 Research and Markets ... the "Global Facial Recognition Market 2015-2019" report ... Facial recognition is a technology used ... the overall facial feature of a person such as ...
(Date:12/11/2014)...  That blood pressure plays a role in human health has ... term for high blood pressure – was first described as ... that,s used in measuring blood pressure was invented in 1896. ... hypertension, its triggers and its effects. In fact, recent findings ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4
... Advanced Synthetic Aperture Radar (ASAR) instrument, shows the breaking away ... West Antarctica. Spanning 34 km in length by 20 km ... the size of Greater London. The animation highlights the ... The Pine Island Glacier is visible stretching from the right ...
... potential to track one,s DNA to a specific country, ... alluring. But while the popularity of genetic ancestry testing ... technology is flawed and could spawn unwelcome societal consequences, ... University of California, Berkeley. "Because race has such ...
... climate change" How will different kinds of businesses -- ... tourism -- be affected" Which state and local governments ... these groups obtain the kinds of climate-change forecasts and ... uncertain decades to come" Find out at "Climate Information: ...
Cached Biology News:Birth of an iceberg 2Birth of an iceberg 3Genetic ancestral testing cannot deliver on its promise, study warns 2Genetic ancestral testing cannot deliver on its promise, study warns 3Genetic ancestral testing cannot deliver on its promise, study warns 4Meeting real-world climate information needs of business, government 2
...
...
... III) from E. coli is an endoribonuclease ... dsRNA fragments that have 2-base, 3'-overhangs. ... fragments of 12-15 bp. RNase III ... ribonucleotides as well as DuraScript™ RNA, produced ...
Mouse monoclonal [6911] to E. coli K99 pilus ( Abpromise for all tested applications)....
Biology Products: