Navigation Links
ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S.
Molecular Devices Corporation, 8/00


INTRODUCTION
Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell number.ATP measurements are used to monitor raw materials and manufacturing planthygiene for bacterial contamination in the food, drug, health care and personalhealthcare industries, as well as for waste water analysis.1 In the biotechnologyand pharmaceutical industries, ATP measurements are used to evaluate cellproliferation and cytotoxicity. The bioluminescent assay of ATP has become verypopular because of its high sensitivity and convenience. In this application note,we show the ATP assay results obtained with the Molecular Devices Lmaxmicroplate luminometer.


PRINCIPLE OF
ASSAY
The basis for the assay is the reaction catalyzed by luciferase, obtained from the common American firefly (Photinus pyralis).2 The enzyme catalyzes the ATP-dependent oxidation of luciferin with the concomitant release of light (Figure 1).When ATP is the limiting component in the reaction, the amount of light emittedis proportional to the concentration of ATP. The intensity of the emitted light canbe measured easily with the Lmax microplate luminometer.


EQUIPMENT AND MATERIALS
1. Lmax microplate luminometer with SOFTmax PRO for Lmax (Molecular Devices Corp.)

2. ENLITENTM ATP Assay System, Cat. No. FF2000; Promega Corporation. 608277-2516. The kit contains Luciferase/Luciferin (L/L) reagent, ATP standard 10-7 M in HEPES buffer, pH 7.75) and ATP-free water.

3. Solid white 96-well microplates; e.g. Porvair PS white, E&G Wallac Cat. # 204003; Tel: 1-800-221-9650; or CorningCostar Cat. No.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:8/21/2014)... including DESY scientists has observed tiny quantum vortices in ... the journal Science that the exotic vortices ... It is the first time that the quantum vortices, ... what is known as superfluid helium, have been detected ... says Andrey Vilesov of the University of Southern California, ...
(Date:8/21/2014)... August 21, 2014 ... has announced the addition of the  "Global ... their offering.       (Logo: http://photos.prnewswire.com/prnh/20130307/600769) ... therapy, is a means of replacing hormones ... the body. This type of therapy is ...
(Date:8/21/2014)... 21, 2014 Telomere Biosciences, LLC (Telomere ... "TELO-20 for Dogs" with Telomerase Activation Complex ... the world for dogs. Telomeres are the protective ... the body. A wealth of ground-breaking research by ... Science and Aging, including Nobel laureates, has demonstrated ...
(Date:8/21/2014)... Mass. , Aug. 21, 2014  Decision ... Brazil and ... The uptake of several newly approved agents, the ... this indication and the launch of emerging biologics ... markets. In particular, the emerging IL-5 inhibitors will ...
Breaking Biology Technology:Scientists observe quantum vortices in cold helium droplets 2Global Hormone Replacement Therapy Market 2014-2018: Key Vendors are Actavis, Bayer, Merck & Co, Novartis and Novo Nordisk 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 3Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3
... ... extend the life of the International Space Station (ISS) to 2020 or beyond. , ... (Vocus) February 1, 2010 -- Astrogenetix ... Space Station (ISS) to 2020 or beyond. , ,Thomas B. Pickens III, Chairman of Astrogenetix, ...
... Feb. 1 Zimmer Holdings, Inc. (NYSE: ... OMeGA Medical Grants Association LLC (OMeGA) $2.175 million in ... as a third party administrator for graduate medical education.  Zimmer was ... funding for educational grants for the 2009-2010 academic year; the Company ...
... , , TSX Exchange Symbol: RVX ... ("Resverlogix" or the "Company") (TSX:RVX) announced today that the Board ... of Bayer HealthCare and Chairman of the Bayer HealthCare Executive ... , Mr. Higgins joined Bayer in 2004 as Chairman of ...
Cached Biology Technology:Astrogenetix Statement On Nasa's Fiscal Year 2011 Budget Announcement 2Zimmer Holdings Announces 2010 Funding for OMeGA Medical Grants Association 2Zimmer Holdings Announces 2010 Funding for OMeGA Medical Grants Association 3Arthur J. Higgins, CEO of Bayer HealthCare, Appointed to Resverlogix Board of Directors 2Arthur J. Higgins, CEO of Bayer HealthCare, Appointed to Resverlogix Board of Directors 3Arthur J. Higgins, CEO of Bayer HealthCare, Appointed to Resverlogix Board of Directors 4
(Date:8/22/2014)... birds and the bees. But that old expression ... The fertilization process for flowering plants is particularly ... and female reproductive cells. New research from an ... and including Carnegie,s Wolf Frommer, David Ehrhardt, and ... process that guides flowering plant fertilization. It is ...
(Date:8/21/2014)... 21, 2014 Nxt-ID, Inc. (OTCQB: NXTD), a ... market, announced today that its shares of common stock ... issued in its proposed underwritten public offering of common ... The Nasdaq Capital Market, subject to closing of its ... warrants, and will trade under the symbol "NXTD" and ...
(Date:8/21/2014)... 21, 2014 /PRNewswire-iReach/ -- LiveScan Service Provider  ... to a partnership with Gabriel Health Institute, one ... growing Certified Nursing Assistant preparatory schools. The ... exclusive member of Binary,s LiveScan service provider,s owner/operator ... improve its service capabilities by widening the ...
Breaking Biology News(10 mins):Calcium and reproduction go together 2Nxt-ID Approved for NASDAQ Capital Market Listing 2Nxt-ID Approved for NASDAQ Capital Market Listing 3LiveScan Provider Binary Biometrics Announces Partnership with Preeminent CNA School, Gabriel Health Institute to Expand Services in Orlando Region 2LiveScan Provider Binary Biometrics Announces Partnership with Preeminent CNA School, Gabriel Health Institute to Expand Services in Orlando Region 3
... computed tomography (CT) evaluation of King Tutankhamun’s mummy believe ... pharaoh died. The CT images and results of their ... the Radiological Society of North America (RSNA). , Ashraf ... University in Egypt, was part of an international team ...
... elongated neck has long been used in textbooks as ... common example has received very little experimental attention. In ... the Mammal Research Institute in the Department of Zoology ... foraging competition with shorter herbivores could explain why giraffes ...
... new ultrasound technique allows radiologists to accurately distinguish ... researchers correctly identified both cancerous and harmless lesions ... findings were presented today at the annual meeting ... , “In our work, elasticity imaging has been ...
Cached Biology News:Radiologists attempt to solve mystery of Tut's demise 2Elasticity imaging identifies cancers and reduces breast biopsies 2
... Mung Bean Nuclease catalyzes the degradation ... yield 5-phosphoryl terminated products. While the nuclease ... very high concentrations the enzyme degrades double-stranded ... Nuclease has been used for transcript mapping ...
... The S.c. EasyComp Transformation Kit is ... Saccharomyces cerevisiae cells. Advantages over commonly ... formation or LiCl methods include:- Cells can ... 30 minutes - Competent cell preparation and ...
Endomorphin-2. Cross reactivity: Endomorphin-1 3%, Met-enkephalin <0.03%, Leu-enkephalin <0.01% and beta-Endorphin <0.01%....
...
Biology Products: