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ANALYSIS OF AMINO ACIDS BY REVERSED PHASE CHROMATOGRAPHY WITH PRECOLUMN DERIVATISATION AND UV/VISIBLE DETECTION

V absorbance @ λ=338nm


Precolumn reaction and analysis conditions:


5L borate buffer + 5L sample ◊ mix 30s
+ 1L OPA reagent ◊ mix 90s
+ 64L H2O ◊ mix 30s ◊ inject.


Gradient program:


We supply Analytical Columns and Guard Column kits upon request.


References:
Bartolomeo MP, Maisano F. Validation of a Reversed-Phase HPLC Method for Quatitative Amino Acid Analysis. J Biol Tech 2006; 17(2):131-137


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