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AMPURE PURIFICATION SYSTEM

for smaller PCR amplicons, such as the 165bp fragment shown in Figure 2. HIGH PURITY HIGH PERFORMANCE
AMPure-purified samples are ideal sequencing templates. The table below shows sequencing results from a single 976bp PCR template purified using AMPure or ExoSAP-IT. The average Phred20 read length and average pass rate for the AMPure-purified samples were 701 and 100% respectively. ExoSAP-IT, an alternative approach of inactivating polymerases and degrading unincorporated primers following PCR thermo-cycling, produces samples with lower purity due to the presence of salts and degraded enzymes and primers. Sequence results following ExoSAP-IT digestions are consistently inferior, with fewer Phred20 bases per read and lower pass rates. Figure 3 exhibits the superior sequencing performance attained using AMPure. RELIABLE RESULTS
AMPure produces reliable recoveries of both large and small amplicons. Figure 4 shows two gels following AMPure purification of 967 bp and 165 bp PCR fragments. SUPERIOR PERFORMANCE
AMPure demonstrates excellent recovery rates of both small and large amplicons while maintaining high sample purity. AMPure samples have little to no contamination as shown by the lack of smearing above the bands (Figure 5). In contrast, alternative purification products have decreased recovery rates and/or lower sample purity, which affect the performance of downstream applications such as sequencing.

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