Navigation Links
AMPURE PURIFICATION SYSTEM

AMPure is a high recovery, SPRI-based PCR clean up system capable of purifying amplicons as small as 100bp. AMPure yields high quality PCR amplicons using a simple, three-step protocol requiring no centrifugation or filtration steps. The protocol can be easily automated or performed manually in both 96 and 384-well formats.

EFFICIENT PCR PURIFICATION
AMPure provides:

  • Efficient removal of unincorporated dNTPs, primers and salts
  • High recovery of small and large amplicons
  • High quality PCR amplicons


  • FLEXIBLE PURIFICATION
    AMPure is flexible enough to be performed in a variety of formats for an array of applications:
    PLATFORMS: APPLICATIONS:
  • 96-well
  • PCR Sequencing
  • 384-well
  • cDNA labeling
  • Fully automated
  • Rolling Circle Amplification
  • Manual





    EFFECTIVE CLEAN UP
    AMPure removes unincorporated primers, dNTPs and salts used during PCR amplification. These products can interfere with downstream applications. AMPure routinely has recovery rates greater than 90% for amplicons larger than 200bp and greater than 85% for smaller PCR amplicons, such as the 165bp fragment shown in Figure 2. HIGH PURITY HIGH PERFORMANCE
    AMPure-purified samples are ideal sequencing templates. The table below shows sequencing results from a single 976bp PCR template purified using AMPure or ExoSAP-IT. The average Phred20 read length and average pass rate for the AMPure-purified samples were 701 and 100% respectively. ExoSAP-IT, an alternative approach of inactivating polymerases and degrading unincorporated primers following PCR thermo-cycling, produces samples with lower purity due to the presence of salts and degraded enzymes and primers. Sequence results following ExoSAP-IT digestions are consistently inferior, with fewer Phred20 bases per read and lower pass rates. Figure 3 exhibits the superior sequencing performance attained using AMPure. RELIABLE RESULTS
    AMPure produces reliable recoveries of both large and small amplicons. Figure 4 shows two gels following AMPure purification of 967 bp and 165 bp PCR fragments. SUPERIOR PERFORMANCE
    AMPure demonstrates excellent recovery rates of both small and large amplicons while maintaining high sample purity. AMPure samples have little to no contamination as shown by the lack of smearing above the bands (Figure 5). In contrast, alternative purification products have decreased recovery rates and/or lower sample purity, which affect the performance of downstream applications such as sequencing.
    COMPLETE AMPURE AUTOMATION
    The lack of centrifugation and filtration steps allows AMPure protocols to be completely automated while maintaining superior performance. AMPure is fully compatible with most commonly used low, medium and high throughout liquid handling systems. Contact Agencourt for the most up-to-date list of supported platforms.

    AMPure has been successfully automated on the Biomek FX from Beckman Coulter in 96 and 384-well formats. (Photo courtesy of Beckman Coulter.)



    '"/>


  • Source:


    Page: All 1 2 3

    Related biology technology :

    1. COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION
    2. SPRINTPREP SINGLE STEP PLASMID PURIFICATION
    Post Your Comments:
    *Name:
    *Comment:
    *Email:


    (Date:2/8/2016)... February 8, 2016 ... Limited, an innovation-driven oncology company developing next generation ... toxic, today announced that chairman emeritus of Tata ... in the company as part of the first ... existing investors Navam Capital and Aarin Capital. ...
    (Date:2/8/2016)... 8, 2016  BioElectronics Corporation (OTC Pink: BIEL), ... today that it is responding to a notice ... Securities and Exchange Commission posted on the agency ... of the Board of BioElectronics Corporation and the ... at The Fuqua School of Business, Duke University.   ...
    (Date:2/8/2016)... ... February 08, 2016 , ... Franz Inc. , an ... technology, today announced the availability of AllegroGraph 6, the leading Semantic Graph Database ... Certified Technology Program (CCPT). AllegroGraph is the first Semantic Graph Database to ...
    (Date:2/8/2016)... that its new website has gone live. On Thursday, Feb. 4, the company unveiled a re-envisioned ... Visit the new site: www.diplomat.is ... ... Diplomat Specialty Pharmacy ... "The goal was to reimagine the website and create a smarter, more comprehensive digital platform," said ...
    Breaking Biology Technology:
    (Date:2/1/2016)... , February 1, 2016 Rising ... to drive global touchfree intuitive gesture control market ... Rising sales of consumer electronics coupled with new ... market size through 2020   ... coupled with new technological advancements to drive global touchfree ...
    (Date:1/25/2016)... Jan. 25, 2016   Unisys Corporation (NYSE: UIS ... F. Kennedy (JFK) International Airport, New York City ... identify imposters attempting to enter the United States ... them. pilot testing of the system at Dulles ... terminals at JFK during January 2016. --> pilot testing ...
    (Date:1/20/2016)... , Jan. 20, 2016 A market ... to directly benefit from the explosion in genomics knowledge. ... Howe Sound Research. A range of dynamic trends are ... - personalized medicine - pharmacogenomics - pathogen evolution ... with large markets - greater understanding of the role ...
    Breaking Biology News(10 mins):