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A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors)

rd.

Results and discussion

Results obtained using the proposed hardware configuration are summarized below.

Figure 2 shows the structure of some of the drugs tested in this study. Nevirapine is a NNRTI drug while the others belong to the PI group. MRM transitions for each of the measured analytes are listed in Figure 3.

Figure 4 shows a typical trace obtained on a serum sample spiked with 1 μg/mL of each drug. In these optimized conditions, the total run time was 6 minutes per sample.

Figure 5 illustrates the linearity obtained on calibrator samples with concentrations spanning from 0.1 to 5 μg/mL for each drug.

Figure 6 documents a reproducibility test made over a Serum sample spiked with 0.5 μg/mL of each drug.

Figure 7 shows the trace obtained on a serum sample from a patient treated with a drug cocktail containing Amprenavir and Ritonavir.

Conclusion

The following conclusions were drawn from these results:

The proposed method is fast. The sample-to-sample cycle time is only 6 minutes.

The proposed method is robust. More than 500 sample injections were made with no evidence of column saturation.

Preprogrammed control and operation of the LC peripheral units using the Analyst acquisition software were effective in reducing solvent consumption by precisely controlling the timing of the high-flow rate regimen during the sample clean up phase of the first dimension LC.

This method is fast and easy to perform, with minimal sample preparation without compromising reproducibility, precision and accuracy.

References

1. Volosov A, Alexander C, Ting L, Soldin SJ. Simple rapid method for quantifica
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