Navigation Links
A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors)

rd.

Results and discussion

Results obtained using the proposed hardware configuration are summarized below.

Figure 2 shows the structure of some of the drugs tested in this study. Nevirapine is a NNRTI drug while the others belong to the PI group. MRM transitions for each of the measured analytes are listed in Figure 3.

Figure 4 shows a typical trace obtained on a serum sample spiked with 1 μg/mL of each drug. In these optimized conditions, the total run time was 6 minutes per sample.

Figure 5 illustrates the linearity obtained on calibrator samples with concentrations spanning from 0.1 to 5 μg/mL for each drug.

Figure 6 documents a reproducibility test made over a Serum sample spiked with 0.5 μg/mL of each drug.

Figure 7 shows the trace obtained on a serum sample from a patient treated with a drug cocktail containing Amprenavir and Ritonavir.

Conclusion

The following conclusions were drawn from these results:

The proposed method is fast. The sample-to-sample cycle time is only 6 minutes.

The proposed method is robust. More than 500 sample injections were made with no evidence of column saturation.

Preprogrammed control and operation of the LC peripheral units using the Analyst acquisition software were effective in reducing solvent consumption by precisely controlling the timing of the high-flow rate regimen during the sample clean up phase of the first dimension LC.

This method is fast and easy to perform, with minimal sample preparation without compromising reproducibility, precision and accuracy.

References

1. Volosov A, Alexander C, Ting L, Soldin SJ. Simple rapid method for quantifica
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
2. A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase
3. Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
4. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
5. Comparison of different protein quantitation methods
6. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
7. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
8. Kinetic determination of vitamin B1
9. Fluorescence assay for determination of protease activity
10. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
11. High-throughput gene expression analysis to screen for anti-asthma drugs
Post Your Comments:
(Date:6/29/2015)... ... 30, 2015 , ... This cutting edge standard making is a madatory tool ... peaks, the WAV-8 is the world’s first cuvette-shaped, solid-state filter that lets metrologists and ... range. Previous spectrophotometer calibration standards, such as those made from didymium glass were only ...
(Date:6/29/2015)... , June 29, 2015  AACC, a ... better health through laboratory medicine, is pleased to ... Clinical Chemistry , has increased to 7.9 ... This increase places Clinical Chemistry in ... reflects the significant influence of the research it ...
(Date:6/29/2015)... June 29, 2015   Veracyte, Inc . (Nasdaq: ... field of molecular cytology, today announced that it has ... Top Workplaces list for the second year in a ... solely on an annual survey of Bay Area-company employees. ... the degree to which Veracyte values our employees and ...
(Date:6/26/2015)... /PRNewswire/ - iCo Therapeutics ("iCo" or "the Company") (TSX-V: ... listed in the management information circular dated June 1, ... Meeting of Shareholders, held earlier today, June 26 ... 5 nominees proposed by management were elected as ... Company,s next Annual Meeting of Shareholders or until ...
Breaking Biology Technology:FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 2FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 3AACC's Journal, Clinical Chemistry, Continues to Have Significant Impact on Medical Testing, Healthcare 2AACC's Journal, Clinical Chemistry, Continues to Have Significant Impact on Medical Testing, Healthcare 3Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 2Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 3iCo Therapeutics Announces Voting & Election Results from Annual Meeting of Shareholders 2iCo Therapeutics Announces Voting & Election Results from Annual Meeting of Shareholders 3
... chemists from the University of Valencia (UV) has confirmed that ... remains at Lixus, in Morocco, was pine resin. The scientists ... could be fragments of material used for iron-working. In ... sealed amphora among the remains at Lixus, an ancient settlement ...
... , Feb. 5 Transgenomic, Inc. (OTC Bulletin Board: TBIO) ... will be presenting a corporate overview of the company at the 12th ... 11:30 a.m. EST on Tuesday, February 9, 2010 ... , About Transgenomic, Inc. , Transgenomic, Inc. ...
... and PALO ALTO, Calif. , Feb. 5 ... Philip Haworth , Ph.D., chief executive officer, will deliver a corporate ... Conference.  The conference will be held February 8-9, 2010 at ... will take place at 11:00 a.m. Eastern on Monday, ...
Cached Biology Technology:Chemical analyses uncover secrets of an ancient amphora 2Transgenomic Presents at 12th Annual BIO CEO & Investor Conference 2Silence Therapeutics to Present at 12th Annual BIO CEO & Investor Conference 2Silence Therapeutics to Present at 12th Annual BIO CEO & Investor Conference 3
(Date:6/23/2015)... Minn. , June 23, 2015   ... company that supports the entire spectrum of clinical ... iMedNet ™ , the company,s intuitive, ... been awarded a Silver 2015 Stevie® Award by ... Health Products & Services Website category.  The American ...
(Date:6/18/2015)... June 18, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market announces that its Wocket® ... on "Money on the Mark", scheduled to air on WABC ... June 20 th . The broadcast air- ... 7:00 to 8:00pm EST. NXT-ID, Inc.,s CEO Gino Pereira ...
(Date:6/16/2015)... , June 16, 2015 /CNW Telbec/ - handyem ... centralized around the incorporation of handyem,s HPC-150 portable ... laboratory, the Mo-POD™. This unprecedented model of mobile ... Conference at the Pennsylvania ... June 15 th to 18 th ...
Breaking Biology News(10 mins):MedNet Solutions' iMedNet eClinical Wins Silver Stevie Award 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 2handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 3
... -- A two-year study of college students at ... learn better and develop higher-level skills by participating ... teaching methods. Elsa Snchez and Richard Craig, ... surveyed students enrolled from 2003-2005 in their Plant ...
... -- We love it fresh, canned and frozen. It,s grown ... by the American Society of Horticultural Science, adds up to ... In other words, sweet corn is big business. Fresh ... July, with only ten percent of the annual volume marketed ...
... Two common dietary molecules found in legumes and ... of radiation, researchers from the University of Maryland ... human skin cells and a skin cancer-prone mouse ... damaging radiation found in sunlight, the team reported ...
Cached Biology News:Teamwork improves learning and career success 2How sweet is it? 2Antioxidants could provide all-purpose radiation protection 2Antioxidants could provide all-purpose radiation protection 3
Curcumin is an extract of turmeric root with antioxidant, anti-tumor promoter, and anti-inflammatory properties. Curcumin inhibits the induction of NOS in macrophages....
UGT1A6 (D-20)...
... is the smallest electronic pressure controller available ... Parker configured this unit specifically for the ... Used in carrier gas flow control, microfluidic ... and hydro-dynamic focusing, the OEM-EPC offers external ...
... Thrust Slide is designed for heavy duty ... limited and substantial side-loading is present. Standard ... millions of trouble-free cycles even in high ... or recirculating ball bearings , Optional corrosion ...
Biology Products: