Navigation Links
A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors)

Purpose

In clinical research, the monitoring of protease inhibitor (PI) and non-nucleoside reverse transcriptase inhibitor (NNRTI) antiretroviral drugs is of significant interest due to the importance of assessing inter-individual and intra-individual metabolism variability. To be of practical use, it is essential that the analytical method used to monitor these drugs is fast and easy to perform, with minimal sample preparation, and without compromising precision and accuracy.

Compared with other methods, liquid chromatography (LC) in combination with tandem mass spectrometry (MS/MS) has the potential for simultaneous determination of antiretroviral drugs with greater specificity, lower detection limits and a broader dynamic range. Several approaches have been proposed for a fast and stable LCMS/ MS platform for antiretroviral drug monitoring (1, 2). So far, however, these methods have not adequately addressed crucial LC/MS/MS configuration and sample preparation parameters required to ensure robustness, cost-effectiveness, and long-term viability.

Overview

This application note describes a LC/MS/MS procedure for the simultaneous determination of nine PI and NNRTI antiretroviral drugs that are commonly used in Europe. Using the procedure presented here, these drugs were determined quickly and reproducibly, with minimal sample preparation, operator intervention, and solvent consumption.

Key features

Sensitive and selective Multiple Reaction Monitoring (MRM) scan function for linear quantitative analysis with wide dynamic range

Patented LINAC collision cell for multi-component analysis at reduced MRM dwell times maintaining sensitivity and preventing crosstalk

Rugged and reliable triple quadrupole MS system for maximum uptime

Analyst software control of all periph
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
2. A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase
3. Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
4. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
5. Comparison of different protein quantitation methods
6. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
7. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
8. Kinetic determination of vitamin B1
9. Fluorescence assay for determination of protease activity
10. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
11. High-throughput gene expression analysis to screen for anti-asthma drugs
Post Your Comments:
(Date:3/1/2015)... 01, 2015 Market Publishers Ltd ... quality market research promotion on Internet. MarketPublishers.com is ... worked out by DataGroup Booksellers. , Natalie Aster, ... Ltd, commented on the recent partnership agreement as ... in our team of publishers. Since 1974, the ...
(Date:2/27/2015)... A paper published today, " Financing ... in Science Translational Medicine, demonstrates the ... the risk associated with investing in the treatment ... of funding for developing so-called "orphan" drugs. ... between scientists at the National Center for Advancing ...
(Date:2/27/2015)... -- Pfenex Inc. (NYSE MKT: PFNX), a clinical-stage biotechnology company ... that it will be presenting at CALBIO 2015 at ... at 3pm PST. Patrick Lucy, chief business officer of ... state of the biosimilar industry landscape entitled The ... more information on CALBIO 2015 and The Evolving ...
(Date:2/26/2015)... , Feb. 26, 2015 S&P Capital ... Factual Stock Report coverage on MabVax Therapeutics Holdings Inc ... is a clinical stage biotechnology company focused on the ... medical needs in the treatment of cancer. MabVax has ... on the protective immune responses generated by patients who ...
Breaking Biology Technology:Market Publishers Ltd and DataGroup Booksellers Sign Partnership Agreement 2Market Publishers Ltd and DataGroup Booksellers Sign Partnership Agreement 3New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 2New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 3New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 4S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 2S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 3S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 4S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 5
... AstraZeneca (NYSE:,AZN) today announced that the U.S. Food ... (quetiapine fumarate) Extended-Release,Tablets for the acute treatment of ... and mixed episodes associated with bipolar I,disorder, and ... adjunctive,therapy to lithium or divalproex. SEROQUEL XR is ...
... ENMD ) a clinical-stage pharmaceutical company developing,therapeutics ... today that Thomas H. Bliss, Jr., EntreMed,s Senior ... Company,overview at the 16th Annual BioPartnering Europe Conference, ... United Kingdom. Mr. Bliss, presentation,is scheduled for Monday, ...
... - Ranked Number 7 out of top 20 ... division of Merck KGaA, Darmstadt, Germany, announced,today that it ... employer in the,biotechnology and pharmaceutical industries, ranking number 7 ... "We are honored that the scientific community has ...
Cached Biology Technology:FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 2FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 3FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 4FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 5FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 6FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 7FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 8FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 9EntreMed to Present at BioPartnering Europe Conference 2EntreMed to Present at BioPartnering Europe Conference 3Merck Serono Named as a Top Employer by Science Magazine 2
(Date:2/12/2015)... , Feb. 12, 2015 NXT-ID, Inc. (NASDAQ: ... "Company"), a biometric authentication company focused on the growing ... provisional patent 62/113114 for DISTRIBUTED METHOD AND ... The patent further establishes NXT-ID,s position in the ... property portfolio the ability for multiple devices to collaborate ...
(Date:2/5/2015)... , Feb. 5, 2015  Marken is starting its ... and has launched a new marketing campaign to solidify ... (CLO).  The new campaign focuses on First ... and shipments. The first headline in the ... Marken,s priorities with its client,s priorities. Marken recognizes the ...
(Date:2/5/2015)... OXFORD, Conn. , Jan. 28, 2015 ... ("NXT-ID" or the "Company"), a biometric authentication company focused ... present at the upcoming RedChip Global Online CEO Conference ... Gino Pereira , CEO of ... discussing its lead product, the Wocket smart wallet and ...
Breaking Biology News(10 mins):NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 2NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 3Marken Launches New Patient-Centric Campaign 2CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 2CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 3
... foot ulcers are a common problem for diabetics and are ... leg amputations in these patients. There is currently no effective ... new research offers hope. French researchers found that diabetic ... prolinespecific molecules found in proteinshowed better wound healing over rats ...
... of the species that inhabit the world,s oceans may remain ... species have been described in the last decade than in ... November 15 in the Cell Press publication Current Biology ... of the worlda massive collaborative undertaking by hundreds of experts ...
... college-age video game enthusiasts teach young surgeons-in-training? ... University of Texas Medical Branch at Galveston (UTMB) ... the superior hand-eye coordination and hand skills gained ... needed to perform today,s most technologically-advanced robotic surgeries. ...
Cached Biology News:Arginine and proline enriched diet may speed wound healing in diabetes 2At least one-third of marine species remain undescribed 2Young gamers offer insight to teaching new physicians robotic surgery 2Young gamers offer insight to teaching new physicians robotic surgery 3
Mouse monoclonal antibody raised against a partial recombinant QARS. NCBI Entrez Gene ID = QARS...
Mouse polyclonal antibody raised against a partial recombinant QPCT. NCBI Entrez Gene ID = 25797...
Mouse monoclonal antibody raised against a partial recombinant QPCT. NCBI Entrez Gene ID = QPCT...
... The FUNNY SHAKERS are multi-functional mixing devices. ... device is a solid, heavy-duty durable shaker ... both mixing and vortexing in nature. Regulated ... keypad with digital display to control time, ...
Biology Products: