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A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

ic, but lacking in versa- tility. Therefore, spectrometric detection strategies have recently come into vogue.64, 65 Spectral imaging and multispectral imaging are necessary for the detection of wideband fluorescence emissions of specimens stained with multiple fluorophores. Spectral imaging is the capture, processing, display and interpretation of images with a high number of spectral channels. Multispectral imaging is the simultaneous or serial acquisition of imaging data from two or more discrete spectral bands. The whole spectrum of a fluorescence emission can be determined by using spectral imaging. The overall shape of a spectrum can provide more information than just a few wave- lengths. In principle, spectral imaging is like having a large number of spectrophotometers working in parallel. Preliminary results from a spectrally-resolved version of this instrument have been reported by Constantinou et al.65

ACKNOWLEDGMENTS

Dr. Gang Li would like to express his thanks to Natural Sciences and Engineering Research Council of Canada for the Industrial Research Fellowship he has been awarded for this research project.

REFERENCES

1. A. Lewis, H. Taha, A. Strinkoski, A. Manevitch, R. Khatchatouriants, A. Dekhter, and E. Ammann, "Near- field optics: from subwavelength illumination to nanometric shadowing," Nature Biotechnology 21(11), pp. 1378-1386, 2003.

2. M. Minsky,"Microscopy Apparatus," US Patent 3,013,467 (1957).

3. M. Minsky, "Memoir on inventing the confocal scanning microscope," Scanning 10(4), pp. 128-138, 1988.

4. T. Wilson and C. J. R. Sheppard, T heory and Practice of Scanning Optical Microscopy , Academic Press, London, 1984.


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